ATRIP_DROME
ID ATRIP_DROME Reviewed; 846 AA.
AC Q9VVN4; B6UXA1; B6UXA5; B6UXA6; B6UXA7; B6UXA8; B6UXA9; B6UXB0; B6UXB1;
AC B6UXB2; B6UXB3; B6UXB4; B6UXB5; B6UXB6; B6UXB7; B6UXB8; B6UXB9; B6UXC0;
AC B6UXC1; B6UXC2; B6UXC3; B6UXC4; B6UXC5; B6UXC6; B6UXC7; B6UXC8; B6UXC9;
AC B6UXD0; B6UXD1;
DT 07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=ATR-interacting protein mus304;
DE AltName: Full=Mutagen-sensitive protein 304;
GN Name=mus304; ORFNames=CG7347;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF GLY-600; VAL-695 AND
RP GLY-718.
RC TISSUE=Embryo;
RX PubMed=10733527;
RA Brodsky M.H., Sekelsky J.J., Tsang G., Hawley R.S., Rubin G.M.;
RT "mus304 encodes a novel DNA damage checkpoint protein required during
RT Drosophila development.";
RL Genes Dev. 14:666-678(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS THR-28; HIS-109; LEU-132;
RP VAL-141; PRO-144; ILE-151; HIS-173; ASN-244; ILE-284; 320-THR-ASP-ASN-322
RP DELINS ILE; GLU-333; ASN-341; LEU-357; ASP-382; ALA-408; GLN-409; ILE-499;
RP HIS-549; TYR-647; 657-VAL-SER-658 DEL; SER-664; 668-ASN-PRO-669; SER-713;
RP SER-773; THR-793; PHE-802; THR-803; PRO-820 AND ASP-831.
RC STRAIN=MW11, MW27, MW38, MW56, MW6, MW63, MW9, NC301, NC303, NC304, NC306,
RC NC319, NC322, NC335, NC336, NC350, NC357, NC358, NC359, NC361, NC362,
RC NC375, NC390, NC397, NC399, NC732, NC740, and NC774;
RX PubMed=18984573; DOI=10.1534/genetics.108.093807;
RA Anderson J.A., Gilliland W.D., Langley C.H.;
RT "Molecular population genetics and evolution of Drosophila meiosis genes.";
RL Genetics 181:177-185(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6]
RP FUNCTION.
RX PubMed=12361566; DOI=10.1016/s0960-9822(02)01165-x;
RA Abdu U., Brodsky M., Schuepbach T.;
RT "Activation of a meiotic checkpoint during Drosophila oogenesis regulates
RT the translation of Gurken through Chk2/Mnk.";
RL Curr. Biol. 12:1645-1651(2002).
RN [7]
RP INTERACTION WITH MEI-41.
RX PubMed=14729967; DOI=10.1128/mcb.24.3.1219-1231.2004;
RA Brodsky M.H., Weinert B.T., Tsang G., Rong Y.S., McGinnis N.M., Golic K.G.,
RA Rio D.C., Rubin G.M.;
RT "Drosophila melanogaster MNK/Chk2 and p53 regulate multiple DNA repair and
RT apoptotic pathways following DNA damage.";
RL Mol. Cell. Biol. 24:1219-1231(2004).
CC -!- FUNCTION: DNA damage checkpoint protein required for chromosome break
CC repair and for genomic stability during development.
CC {ECO:0000269|PubMed:10733527, ECO:0000269|PubMed:12361566}.
CC -!- SUBUNIT: Interacts with ATR/mei-41. {ECO:0000269|PubMed:14729967}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:10733527}.
CC -!- TISSUE SPECIFICITY: Highly expressed in the oocyte and nurse cells from
CC stage 5 onward and in embryos prior to during nuclear division 14.
CC Then, it decreases to background levels during interphase 14. Weakly or
CC not expressed in stage embryos and imaginal disks.
CC {ECO:0000269|PubMed:10733527}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC {ECO:0000269|PubMed:10733527}.
CC -!- DOMAIN: The EEXXXDDL motif is required for the interaction with
CC ATR/mei-41. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ATRIP family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF224715; AAF43388.1; -; mRNA.
DR EMBL; FJ219149; ACI96445.1; -; Genomic_DNA.
DR EMBL; FJ219153; ACI96449.1; -; Genomic_DNA.
DR EMBL; FJ219154; ACI96450.1; -; Genomic_DNA.
DR EMBL; FJ219155; ACI96451.1; -; Genomic_DNA.
DR EMBL; FJ219156; ACI96452.1; -; Genomic_DNA.
DR EMBL; FJ219157; ACI96453.1; -; Genomic_DNA.
DR EMBL; FJ219158; ACI96454.1; -; Genomic_DNA.
DR EMBL; FJ219159; ACI96455.1; -; Genomic_DNA.
DR EMBL; FJ219160; ACI96456.1; -; Genomic_DNA.
DR EMBL; FJ219161; ACI96457.1; -; Genomic_DNA.
DR EMBL; FJ219162; ACI96458.1; -; Genomic_DNA.
DR EMBL; FJ219163; ACI96459.1; -; Genomic_DNA.
DR EMBL; FJ219164; ACI96460.1; -; Genomic_DNA.
DR EMBL; FJ219165; ACI96461.1; -; Genomic_DNA.
DR EMBL; FJ219166; ACI96462.1; -; Genomic_DNA.
DR EMBL; FJ219167; ACI96463.1; -; Genomic_DNA.
DR EMBL; FJ219168; ACI96464.1; -; Genomic_DNA.
DR EMBL; FJ219169; ACI96465.1; -; Genomic_DNA.
DR EMBL; FJ219170; ACI96466.1; -; Genomic_DNA.
DR EMBL; FJ219171; ACI96467.1; -; Genomic_DNA.
DR EMBL; FJ219172; ACI96468.1; -; Genomic_DNA.
DR EMBL; FJ219173; ACI96469.1; -; Genomic_DNA.
DR EMBL; FJ219174; ACI96470.1; -; Genomic_DNA.
DR EMBL; FJ219175; ACI96471.1; -; Genomic_DNA.
DR EMBL; FJ219176; ACI96472.1; -; Genomic_DNA.
DR EMBL; FJ219177; ACI96473.1; -; Genomic_DNA.
DR EMBL; FJ219178; ACI96474.1; -; Genomic_DNA.
DR EMBL; FJ219179; ACI96475.1; -; Genomic_DNA.
DR EMBL; AE014296; AAF49277.2; -; Genomic_DNA.
DR EMBL; AY118585; AAM49954.1; -; mRNA.
DR RefSeq; NP_524135.3; NM_079411.4.
DR AlphaFoldDB; Q9VVN4; -.
DR SMR; Q9VVN4; -.
DR BioGRID; 65288; 38.
DR IntAct; Q9VVN4; 4.
DR STRING; 7227.FBpp0074888; -.
DR PaxDb; Q9VVN4; -.
DR EnsemblMetazoa; FBtr0075122; FBpp0074888; FBgn0002901.
DR GeneID; 40003; -.
DR KEGG; dme:Dmel_CG7347; -.
DR CTD; 40003; -.
DR FlyBase; FBgn0002901; mus304.
DR VEuPathDB; VectorBase:FBgn0002901; -.
DR eggNOG; ENOG502SDTQ; Eukaryota.
DR HOGENOM; CLU_335948_0_0_1; -.
DR InParanoid; Q9VVN4; -.
DR OMA; GCECYVK; -.
DR OrthoDB; 295577at2759; -.
DR PhylomeDB; Q9VVN4; -.
DR Reactome; R-DME-176187; Activation of ATR in response to replication stress.
DR Reactome; R-DME-5693607; Processing of DNA double-strand break ends.
DR Reactome; R-DME-6804756; Regulation of TP53 Activity through Phosphorylation.
DR Reactome; R-DME-69473; G2/M DNA damage checkpoint.
DR SignaLink; Q9VVN4; -.
DR BioGRID-ORCS; 40003; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 40003; -.
DR PRO; PR:Q9VVN4; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0002901; Expressed in egg cell and 21 other tissues.
DR Genevisible; Q9VVN4; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0000077; P:DNA damage checkpoint signaling; IMP:FlyBase.
DR GO; GO:0006281; P:DNA repair; IMP:FlyBase.
DR GO; GO:0016321; P:female meiosis chromosome segregation; IMP:FlyBase.
DR GO; GO:0007143; P:female meiotic nuclear division; IMP:FlyBase.
DR GO; GO:0007444; P:imaginal disc development; TAS:FlyBase.
DR GO; GO:0033314; P:mitotic DNA replication checkpoint signaling; IMP:FlyBase.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:FlyBase.
DR GO; GO:0007348; P:regulation of syncytial blastoderm mitotic cell cycle; IMP:FlyBase.
DR GO; GO:0009314; P:response to radiation; TAS:FlyBase.
DR GO; GO:0000723; P:telomere maintenance; IGI:FlyBase.
DR InterPro; IPR033349; ATRIP.
DR PANTHER; PTHR28594; PTHR28594; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; DNA damage; DNA repair; Reference proteome.
FT CHAIN 1..846
FT /note="ATR-interacting protein mus304"
FT /id="PRO_0000226819"
FT REGION 20..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 90..109
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 135..162
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 651..681
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 168..240
FT /evidence="ECO:0000255"
FT COILED 327..359
FT /evidence="ECO:0000255"
FT MOTIF 504..510
FT /note="EEXXXDL motif"
FT COMPBIAS 139..162
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 28
FT /note="A -> T (in strain: MW56, NC335, NC336, NC362, NC740
FT and NC774)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 109
FT /note="P -> H (in strain: MW38)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 132
FT /note="M -> L (in strain: MW38)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 141
FT /note="E -> V (in strain: MW27)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 144
FT /note="T -> P (in strain: MW11, MW27, MW38, MW56, MW6,
FT MW63, MW9, NC301, NC322, NC335, NC336, NC350, NC357, NC362,
FT NC390, NC397, NC397, NC732, NC740 and NC774)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 151
FT /note="T -> I (in strain: NC358)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 173
FT /note="Q -> H (in strain: MW9 and NC350)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 244
FT /note="D -> N (in strain: NC350)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 284
FT /note="M -> I (in strain: MW11, MW27, MW6 and MW63)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 320..322
FT /note="TDN -> I (in NC319, NC335 and NC399)"
FT VARIANT 333
FT /note="D -> E (in strain: MW38, MW9, NC319, NC335, NC336,
FT NC359, NC362 and NC399)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 341
FT /note="H -> N (in strain: MW38, MW9, NC319, NC335, NC336,
FT NC359, NC362 and NC399)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 357
FT /note="Q -> L (in strain: NC304)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 382
FT /note="A -> D (in strain: MW56)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 408
FT /note="V -> A (in strain: NC359 and NC740)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 409
FT /note="H -> Q (in strain: MW56)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 499
FT /note="L -> I (in strain: MW63 and NC306)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 549
FT /note="P -> H (in strain: MW27)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 647
FT /note="F -> Y (in strain: MW11, MW27, MW38, MW6, MW9,
FT NC303, NC304, NC306, NC322, NC335, NC336, NC350, NC390,
FT NC397, NC732, NC740 and NC774)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 657..658
FT /note="Missing (in strain: MW9)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 664
FT /note="A -> S (in strain: NC304, NC335, NC350, NC397, NC732
FT and MW9)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 668..669
FT /note="Missing (in strain: MW11, MW27, MW6, MW9 and NC774)"
FT VARIANT 713
FT /note="G -> S (in strain: MW9)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 773
FT /note="L -> S (in strain: NC303, NC319 and NC361)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 793
FT /note="A -> T (in strain: MW11, MW27, MW38, MW56, MW9,
FT NC390 and NC740)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 802
FT /note="L -> F (in strain: MW11 and NC390)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 803
FT /note="S -> T (in strain: MW6)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 820
FT /note="S -> P (in strain: MW6)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT VARIANT 831
FT /note="N -> D (in strain: MW27, MW38, MW56, MW63, MW9,
FT NC304, NC322, NC335, NC336, NC357, NC358, NC362, NC397,
FT NC399, NC732 and NC774)"
FT /evidence="ECO:0000269|PubMed:18984573"
FT MUTAGEN 600
FT /note="G->E: In mus304-4; induces abnormal bristle
FT morphology, decreased meiotic recombination and arrested
FT embryonic development."
FT /evidence="ECO:0000269|PubMed:10733527"
FT MUTAGEN 695
FT /note="V->E: In mus304-3; induces abnormal bristle
FT morphology, decreased meiotic recombination and arrested
FT embryonic development."
FT /evidence="ECO:0000269|PubMed:10733527"
FT MUTAGEN 718
FT /note="G->D: In mus304-6; induces abnormal bristle
FT morphology, decreased meiotic recombination and arrested
FT embryonic development."
FT /evidence="ECO:0000269|PubMed:10733527"
SQ SEQUENCE 846 AA; 96119 MW; B0BD8F84DB4B3D79 CRC64;
MAKRFSAMKD FARCKKPRLD VSVTRGSARP SPPRNNFDGI LWDDDDDVIL MATQLAEAEI
EAEERKKKGG TEVDIGNSEV TFSEFAPTFQ GSTSTQQMFP PPPPPQKKPT SLDMDAIFAD
DDDFDFLAVT LMDSEPQKMP EPKTSTSRIT TSSISVQQKT TTTTTINATQ SRQQEHQLKF
LMDRIEALKR ENAQLEKNLG DSKERNEIKS GEVSLLRDEL KHLRQQLQAS KMEKLALADE
TNRDCNKKVA EAAKQIAAKD IELKIKNAEF SKLKTQQKAH ERSMNSSMSI LQAAPDPLEK
RLSLRLNRLN IHRSVPGLKT DNGSVFEYSE NEDQTKKRRN HFELELKQLL LHYARLQAKP
ESVDNLLPRI LSSVGKVFTE FASYAQSLDF PHNCMLYPYN PHNLEEEVHR ISLTHQSCLY
DNEKAVPLRR FIATLALICR REERISRGLT EWKENDLGLL DMAIEAITKL GFSYEVGQHF
GLLEALTSLL NSLLQENALL QHNEELLFDL LKQLVFTRPS PWVFAELSSC FLSCLRHPQL
MDKMCVNSPK DCFVSDRVRS VYRFGPDSCL LQVYAGLLEL CFFSETPLRQ DYFQLLLKIG
GNHVRFAFEC FKNPPDFILE MLPYFADDGD EDSSDGTLMK TGTSLSFNST GAVQGSVSNG
STSASVSNPN QNSNSSTTQR GKGCECYVKL CLSAVTIVFQ VMHQWMLHSR KAGTEEVGEI
SRIAVHLLSL VFHEYYLTCL FRDSEETTKH YLSLICNWWS EHANLLGFQS IHLRLLNQLV
KAHFMLKPLH LEAKPNNPVN DLSEWKRIVK NADDQRAVKS AVTVDPSKLL NTDFFSALKR
EENTFE
