RPOB_PSE14
ID RPOB_PSE14 Reviewed; 1357 AA.
AC Q48D29;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=PSPPH_4599;
OS Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)
OS (Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=264730;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1448A / Race 6;
RX PubMed=16159782; DOI=10.1128/jb.187.18.6488-6498.2005;
RA Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R.,
RA Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M.,
RA Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J.,
RA Creasy T., Davidsen T.M., Haft D.H., Zafar N., Zhou L., Halpin R.,
RA Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M.,
RA Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A.,
RA Buell R.;
RT "Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola
RT 1448A reveals divergence among pathovars in genes involved in virulence and
RT transposition.";
RL J. Bacteriol. 187:6488-6498(2005).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000058; AAZ37001.1; -; Genomic_DNA.
DR RefSeq; WP_011169631.1; NC_005773.3.
DR AlphaFoldDB; Q48D29; -.
DR SMR; Q48D29; -.
DR STRING; 264730.PSPPH_4599; -.
DR EnsemblBacteria; AAZ37001; AAZ37001; PSPPH_4599.
DR KEGG; psp:PSPPH_4599; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_6; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000000551; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1357
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000224096"
SQ SEQUENCE 1357 AA; 151004 MW; DF00AF9DFE5A7F2F CRC64;
MAYSYTEKKR IRKDFSKLPD VMDVPYLLAI QLDSYREFLQ AGATKDQFRD VGLHAAFKSV
FPIISYSGNA ALEYVGYRLG EPAFDVKECV LRGVTYAVPL RVKVRLIIFD KESSNKAIKD
IKEQEVYMGE IPLMTENGTF VINGTERVIV SQLHRSPGVF FDHDRGKTHS SGKLLYSARI
IPYRGSWLDF EFDPKDCVFV RIDRRRKLPA SVLLRALGYT TEQVLDAFYT TNVFHVRGEN
LSLELVPQRL RGEIAVLDIL DDKGKVIVEQ GRRITARHIN QLEKAGIKEL EVPLDYVLGR
TTAKVIVHPA TGEIIAECNT ELNTEILAKI AKAQVVRIET LYTNDIDCGP FVSDTLKIDS
TSNQLEALVE IYRMMRPGEP PTKDAAETLF NNLFFSPERY DLSAVGRMKF NRRIGRTEIE
GSGVLCKEDI VAVLKTLVDI RNGKGIVDDI DHLGNRRVRC VGEMAENQFR VGLVRVERAV
KERLSMAESE GLMPQDLINA KPVAAAVKEF FGSSQLSQFM DQNNPLSEIT LKRRVSALGP
GGLTRERAGF EVRDVHPTHY GRVCPIETPE GPNIGLINSL AAYARTNQYG FLESPYRVVK
EGLVTEEIVF LSAIEEADHV IAQASAAMND KQELIDELVA VRHLNEFTVK APADVTLMDV
SPKQVVSVAA SLIPFLEHDD ANRALMGSNM QRQAVPTLRA DKPLVGTGME RNVARDSGVC
VVARRGGVID SVDASRIVVR VADDEVETGE AGVDIYNLTK YTRSNQNTCI NQRPLVSKGD
RVQRSDIMAD GPSTDMGELA LGQNMRIAFM AWNGFNFEDS ICLSERVVQE DRFTTIHIQE
LTCVARDTKL GPEEITADIP NVGEAALNKL DEAGIVYVGA EVGAGDILVG KVTPKGETQL
TPEEKLLRAI FGEKASDVKD TSLRVPTGTK GTVIDVQVFT RDGVERDARA LSIEKSQLDE
IRKDLNEEFR IVEGATFERL RSALVGRVAE GGAGLKKGQE ITNEVLDGLE HGQWFKLRMA
EDALNEQLEK AQAYIVDRRR LLDDKFEDKK RKLQQGDDLA PGVLKIVKVY LAIRRRIQPG
DKMAGRHGNK GVVSVIMPVE DMPHDANGTP VDIVLNPLGV PSRMNVGQIL ETHLGLAAKG
LGEKINRMLE EQRKVAELRK FLNEIYNEIG GRQESLEDLT DNEILDLAKN LRNGVPMATP
VFDGAKESEI KAMLKLADMP ESGQMQLFDG RTGNKFERAV TVGYMYMLKL NHLVDDKMHA
RSTGSYSLVT QQPLGGKAQF GGQRFGEMEV WALEAYGAAY TLQEMLTVKS DDVNGRTKMY
KNIVDGDHRM EPGMPESFNV LIKEIRSLGI DIDLETE