ID   RPOB_PSEA7              Reviewed;        1357 AA.
AC   A6UZI1;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=PSPA7_0830;
OS   Pseudomonas aeruginosa (strain PA7).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=381754;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PA7;
RA   Dodson R.J., Harkins D., Paulsen I.T.;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000744; ABR81670.1; -; Genomic_DNA.
DR   RefSeq; WP_012074226.1; NC_009656.1.
DR   AlphaFoldDB; A6UZI1; -.
DR   SMR; A6UZI1; -.
DR   EnsemblBacteria; ABR81670; ABR81670; PSPA7_0830.
DR   KEGG; pap:PSPA7_0830; -.
DR   HOGENOM; CLU_000524_4_0_6; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000001582; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase.
FT   CHAIN           1..1357
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_1000051973"
SQ   SEQUENCE   1357 AA;  150866 MW;  F5DB941C819394BD CRC64;
     MAYSYTEKKR IRKDFSKLPD VMDVPYLLAI QLDSYREFLQ AGATKEQFRD IGLHAAFKSV
     FPIISYSGNA ALEYVGYRLG EPAFDVKECV LRGVTFAVPL RVKVRLIIFD RESSNKAIKD
     IKEQEVYMGE IPLMTENGTF IINGTERVIV SQLHRSPGVF FDHDRGKTHS SGKLLYSARI
     IPYRGSWLDF EFDPKDCVFV RIDRRRKLPA SVLLRALGYS TEEILNAFYA TNVFHIKGET
     LNLELVPQRL RGEVASIDIK DGSGKVIVEQ GRRITARHIN QLEKAGVTQL EVPFDYLIGR
     TIAKAIVHPA TGEIIAECNT ELTLDLLAKV AKAQVVRIET LYTNDIDCGP FISDTLKIDN
     TSNQLEALVE IYRMMRPGEP PTKEAAETLF GNLFFSAERY DLSAVGRMKF NRRIGRTEIE
     GPGVLSKEDI IDVLKTLVDI RNGKGIVDDI DHLGNRRVRC VGEMAENQFR VGLVRVERAV
     KERLSMAESE GLMPQDLINA KPVAAAIKEF FGSSQLSQFM DQNNPLSEIT HKRRVSALGP
     GGLTRERAGF EVRDVHPTHY GRVCPIETPE GPNIGLINSL ATYARTNKYG FLESPYRVVK
     DSLVTDEIVF LSAIEEADHV IAQASATLNE KGQLVDELVA VRHLNEFTVK APEDVTLMDV
     SPKQVVSVAA SLIPFLEHDD ANRALMGSNM QRQAVPTLRA DKPLVGTGME RNVARDSGVC
     VVARRGGVID SVDASRVVVR VADDEVETGE AGVDIYNLTK YTRSNQNTCI NQRPLVSKGD
     VVARGDILAD GPSTDMGELA LGQNMRVAFM PWNGFNFEDS ICLSERVVQE DRFTTIHIQE
     LTCVARDTKL GPEEITADIP NVGEAALNKL DEAGIVYVGA EVQAGDILVG KVTPKGETQL
     TPEEKLLRAI FGEKASDVKD TSLRVPTGTK GTVIDVQVFT RDGVERDSRA LSIEKMQLDQ
     IRKDLNEEFR IVEGATFERL RAALVGAKAE GGPALKKGTE ITDDYLDGLE RGQWFKLRMA
     DDALNEQLEK AQAYISDRRQ LLDDKFEDKK RKLQQGDDLA PGVLKIVKVY LAIKRRIQPG
     DKMAGRHGNK GVVSVIMPVE DMPHDANGTP VDIVLNPLGV PSRMNVGQIL ETHLGLAAKG
     LGEKINRMLE EQRKVAELRK FLHEIYNEIG GREENLDELG DNEILALAKN LRGGVPMATP
     VFDGAKEREI KAMLKLADLP ESGQMRLFDG RTGNQFERPT TVGYMYMLKL NHLVDDKMHA
     RSTGSYSLVT QQPLGGKAQF GGQRFGEMEV WALEAYGAAY TLQEMLTVKS DDVNGRTKMY
     KNIVDGDHRM EAGMPESFNV LIKEIRSLGI DIELETE