RPOB_PSELT
ID RPOB_PSELT Reviewed; 1172 AA.
AC A8F4G0;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Tlet_0477;
OS Pseudothermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / NBRC 107922 /
OS TMO) (Thermotoga lettingae).
OC Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Pseudothermotoga.
OX NCBI_TaxID=416591;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-301 / DSM 14385 / NBRC 107922 / TMO;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Foster B., Bruce D., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Mikhailova N., Nelson K., Gogarten J.P., Noll K.,
RA Richardson P.;
RT "Complete sequence of Thermotoga lettingae TMO.";
RL Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000812; ABV33044.1; -; Genomic_DNA.
DR RefSeq; WP_012002525.1; NC_009828.1.
DR AlphaFoldDB; A8F4G0; -.
DR SMR; A8F4G0; -.
DR STRING; 416591.Tlet_0477; -.
DR PRIDE; A8F4G0; -.
DR EnsemblBacteria; ABV33044; ABV33044; Tlet_0477.
DR KEGG; tle:Tlet_0477; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_3_0; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000002016; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1172
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000329194"
SQ SEQUENCE 1172 AA; 131439 MW; C2D15F24BF19FAC9 CRC64;
MRTVCYGKRE RLTFGRIHDA VKVPNLISIQ IDSYKDFLEN GLIEVLKKFS PITSQPHKGD
LKKGEKGFVL EFVSTKVGQP NAPVEECKQK GLTYTVPVYT TVRITDVNTG EMREEEAFLG
SIPHMTENGT FIINGAERVI VSQLVRAPGV YFVDEIPKTQ VVSPIYVAHF LPVRGAWLEL
LYYSADNMFY ARIDRKRRIN LFLLFKALGF TDDLEILDLF PDPIDADDEY TMEKAVGSII
LHDVVLDGKK IVERGGVLTK AASQAILESK IATVVRAHPA AQKTLEKMVD TYGEVDSSKA
YVEIFRKLKP GEIPRVNTAK AYLTSLYFST ERFELSDVGR YKINKRLTQA YRRYLAQVKG
LPEEEVESVE YNPEDPVLTP MDIVLASRYL LDVSKNPEIM DTKDHLGNKR VRTVGELIKL
EFERAFARAQ RLIQERLTLY ASLDKISIQS LINVKTIIAG INQFFATSPL SQFMEQVNPL
AELTHKRRLT AVGPGGLKRE RARFEVRDVH HSHYGRMCPI ETPEGANIGL ITSLAVYANI
DKFGFLTTPY RKVVNGVVTS EIVYLTADEE EHYNIAPCTI KIDKDGRILD ERVPVRFMER
LQFVEKNKVQ FMDVSTKQIV SVSTSLIPFL EHDDANRALM GSNMQRQAVP LVKPEAPFVA
TGVEYDAALY SGYVVQAKYS GRVKKVDSRK IVIERMDENG KPVLKNGKPV LDEYRLMKFM
RTNQDTTVNQ RPIVDIGDIV KAGDVIADGP ATDMGELALG KNVLVAFMPW EGYNFEDAIL
VSEELLEEDT FTSIHIEVYE TQARDTRLGP EEITVDIPNV SKEALRNLDE NGIVRVGAYV
GPQDILVGKV TPKGEGETTP EEKIIRSVFG ERGKDVKDTS LRLPHGTDGR VIDVKVFDKD
EETDLGAGVN KLVKVYVACR KTLEVGDKLA GRHGNKGVVS KILPKEDMPF LPDGTPVQLV
LSPLGVPSRM NVGQILETHL GWLAKLTRNW FATPVFDGAK ENEILPWLYE ERRAVGLEEG
DSDNEPSGKV VLRDGRTGES FAEPIVVGYI YMMKLVHIAK DKIHARSTGP YSLIHQQPLG
GKAQFGGQRF GEMEVWALEA HGAAHTLNEM LTIKSDDIKG RNEVYKAILK GKNIPEPGIP
ESFRVLIKEL RGLALDIRVY DENGNEIDID RV
