RPOB_PSEP1
ID RPOB_PSEP1 Reviewed; 1357 AA.
AC A5VXP0;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Pput_0480;
OS Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=351746;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700007 / DSM 6899 / BCRC 17059 / F1;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P.;
RT "Complete sequence of Pseudomonas putida F1.";
RL Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000712; ABQ76650.1; -; Genomic_DNA.
DR RefSeq; WP_003255495.1; NC_009512.1.
DR AlphaFoldDB; A5VXP0; -.
DR SMR; A5VXP0; -.
DR STRING; 351746.Pput_0480; -.
DR PRIDE; A5VXP0; -.
DR EnsemblBacteria; ABQ76650; ABQ76650; Pput_0480.
DR KEGG; ppf:Pput_0480; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_6; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1357
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000051975"
SQ SEQUENCE 1357 AA; 150993 MW; 240CE57A342A0C30 CRC64;
MAYSYTEKKR IRKDFSKLPD VMDVPYLLAI QLDSYREFLQ AGASKDHFRD VGLHAAFKSV
FPIISYSGNA ALEYVGYRLG EPAFDVKECV LRGVTFAVPL RVKVRLIIFD KESSNKAIKD
IKEQEVYMGE IPLMTENGTF VINGTERVIV SQLHRSPGVF FDHDRGKTHS SGKLLYSARI
IPYRGSWLDF EFDPKDCVFV RIDRRRKLPA SVLLRALGYS TEEVLNTFYT TNVFHISGEK
LSLELVPQRL RGEVAVMDIH DETGKVIVEQ GRRITARHIN QLEKAGVKQL DVPMEYVLGR
TTAKAIVHPA TGEILAECNT EMTTELLIKV AKAQVVRIET LYTNDIDCGP FISDTLKIDT
TSNQLEALVE IYRMMRPGEP PTKDAAETLF NNLFFSAERY DLSAVGRMKF NRRIGRTEIE
GSGVLSKEDI VEVLKTLVDI RNGKGIVDDI DHLGNRRVRC VGEMAENQFR VGLVRVERAV
KERLSMAESE GLMPQDLINA KPVAAAVKEF FGSSQLSQFM DQNNPLSEIT HKRRVSALGP
GGLTRERAGF EVRDVHPTHY GRVCPIETPE GPNIGLINSL AAYARTNQYG FLESPYRVVK
EGVVSDDIVF LSAIEEADHV IAQASAAMND KKQLIDELVA VRHLNEFTVK APEDVTLMDV
SPKQVVSVAA SLIPFLEHDD ANRALMGSNM QRQAVPTLRA DKPLVGTGME RNVARDSGVC
VVARRGGVID SVDASRIVVR VADDEVETGE AGVDIYNLTK YTRSNQNTCI NQRPLVSKGD
KVQRGDIMAD GPSTDMGELA LGQNMRIAFM AWNGFNFEDS ICLSERVVQE DRFTTIHIQE
LTCVARDTKL GPEEITADIP NVGEAALNKL DEAGIVYVGA EVGAGDILVG KVTPKGETQL
TPEEKLLRAI FGEKASDVKD TSLRVPTGTK GTVIDVQVFT RDGVERDSRA LAIEKMQLDE
IRKDLNEEFR IVEGATFERL RSALNGQVVD GGAGLKKGTV ITDEVLDGLE HGQWFKLRMA
EDALNEQLEK AQQYIVDRRR LLDDKFEDKK RKLQQGDDLA PGVLKIVKVY LAIRRRIQPG
DKMAGRHGNK GVVSVIMPVE DMPHDANGTP VDVVLNPLGV PSRMNVGQIL ETHLGLAAKG
LGEKIDRMLE EQRKAAELRV FLTEVYNEIG GRQENLDEFT DEEILALANN LKKGVPMATP
VFDGAKEREI KAMLKLADLP ESGQMVLFDG RTGNKFERPV TVGYMYMLKL NHLVDDKMHA
RSTGSYSLVT QQPLGGKAQF GGQRFGEMEV WALEAYGAAY TLQEMLTVKS DDVNGRTKMY
KNIVDGDHRM EPGMPESFNV LIKEIRSLGI DIDLETE
