ID   RPOB_PSEPF              Reviewed;        1357 AA.
AC   Q3K5Y1;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Pfl01_5086;
OS   Pseudomonas fluorescens (strain Pf0-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=205922;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pf0-1;
RX   PubMed=19432983; DOI=10.1186/gb-2009-10-5-r51;
RA   Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R.,
RA   Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M.,
RA   Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J.,
RA   Harris S., Challis G.L., Yaxley A.M., Harris D., Seeger K., Murphy L.,
RA   Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K.,
RA   Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M.,
RA   Parkhill J., Levy S.B., Rainey P.B., Thomson N.R.;
RT   "Genomic and genetic analyses of diversity and plant interactions of
RT   Pseudomonas fluorescens.";
RL   Genome Biol. 10:R51.1-R51.16(2009).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000094; ABA76823.1; -; Genomic_DNA.
DR   RefSeq; WP_007957595.1; NC_007492.2.
DR   AlphaFoldDB; Q3K5Y1; -.
DR   SMR; Q3K5Y1; -.
DR   STRING; 205922.Pfl01_5086; -.
DR   PRIDE; Q3K5Y1; -.
DR   EnsemblBacteria; ABA76823; ABA76823; Pfl01_5086.
DR   KEGG; pfo:Pfl01_5086; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_0_6; -.
DR   OMA; FMTWEGY; -.
DR   Proteomes; UP000002704; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 2.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 2.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase.
FT   CHAIN           1..1357
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000224095"
SQ   SEQUENCE   1357 AA;  150671 MW;  71A2AE9AD2361B71 CRC64;
     MAYSYTEKKR IRKDFSKLPD VMDVPYLLAI QLDSYREFLQ AGATKDQFRD VGLHAAFKSV
     FPIISYSGNA ALEYVGYRLG EPAFDVKECV LRGVTFAVPL RVKVRLIIFD KESSNKAIKD
     IKEQEVYMGE IPLMTENGTF VINGTERVIV SQLHRSPGVF FDHDRGKTHS SGKLLYSARI
     IPYRGSWLDF EFDPKDCVFV RIDRRRKLPA SVLLRALGYT TEEVLDAFYT TNVFHVKGEG
     LSLELVPQRL RGEVAVLDIQ DDKGKVIVEQ GRRITARHIN QLEKAGIKEL DVPLDYVLGR
     TTAKAIVHPA TGEILAECNT ELSTEILAKI AKAGVVRIET LYTNDIDCGP FVSDTLKIDS
     TSNQLEALVE IYRMMRPGEP PTKDAAETLF NNLFFSPERY DLSAVGRMKF NRRIGRTEIE
     GSGVLCKEDI VAVLKTLVDI RNGKGIVDDI DHLGNRRVRC VGEMAENQFR VGLVRVERAV
     KERLSMAESE GLMPQDLINA KPVAAAVKEF FGSSQLSQFM DQNNPLSEIT HKRRVSALGP
     GGLTRERAGF EVRDVHPTHY GRVCPIETPE GPNIGLINSL AAYARTNQYG FLESPYRVVK
     EGVVTDDIVF LSAIEEADHV IAQASATMNE QKVLIDELVA VRHLNEFTVK APEDVTLMDV
     SPKQVVSVAA SLIPFLEHDD ANRALMGSNM QRQAVPTLRA DKPLVGTGME RNVARDSGVC
     VVARRGGVID SVDASRIVVR VADDEVETGE AGVDIYNLTK YTRSNQNTCI NQRPLVSKGD
     RVQRSDIMAD GPSTDMGELA LGQNMRIAFM AWNGFNFEDS ICLSERVVQE DRFTTIHIQE
     LTCVARDTKL GPEEITADIP NVGEAALNKL DEAGIVYVGA EVGAGDILVG KVTPKGETQL
     TPEEKLLRAI FGEKASDVKD TSLRVPTGTK GTVIDVQVFT RDGVERDARA LSIEKSQLDE
     IRKDLNEEFR IVEGATFERL RSALVGHKAE GGAGLKKGQD ITDEVLDGLE HGQWFKLRMA
     EDALNEQLEK AQAYIVDRRR LLDDKFEDKK RKLQQGDDLA PGVLKIVKVY LAIRRRIQPG
     DKMAGRHGNK GVVSVIMPVE DMPHDANGTP VDVVLNPLGV PSRMNVGQIL ETHLGLAAKG
     LGEKINRMVE EQRKVAELRT FLDEIYNQIG GRNEDLDSFS DQEILDLAKN LRGGVPMATP
     VFDGAKESEI KAMLKLADLP ESGQMQLTDG RTGNKFERPV TVGYMYMLKL NHLVDDKMHA
     RSTGSYSLVT QQPLGGKAQF GGQRFGEMEV WALEAYGAAY TLQEMLTVKS DDVNGRTKMY
     KNIVDGDHRM EPGMPESFNV LIKEIRSLGI DIDLETE