ID   RPOB_PSEPU              Reviewed;        1357 AA.
AC   P19175;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 2.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Borodin A.M., Danilkovich A.V., Allikmets R.L., Rostapshov V.M.,
RA   Chernov I.P., Azhikina T.L., Monastyrskaya S., Sverdlov D.;
RT   "Nucleotide sequence of the rpoB gene coding for the beta-subunit of RNA
RT   polymerase in Pseudomonas putida.";
RL   Dokl. Biochem. 302:1261-1265(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1036-1357.
RX   PubMed=3219133;
RA   Borodin A.M., Danilkovich A.V., Chernov I.P., Azhykina T.L.,
RA   Rostapshov V.M., Monastyrskaya G.S.;
RT   "Genes coding for RNA polymerase in bacteria. III. The use of modified
RT   Sanger's method for sequencing the C-terminal region of rpoB gene, N-
RT   terminal region of rpoC gene and intercistron region of RNA polymerase in
RT   Pseudomonas putida.";
RL   Bioorg. Khim. 14:1179-1182(1988).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; X15849; CAA33846.1; -; Genomic_DNA.
DR   EMBL; M38319; AAA25986.1; -; Genomic_DNA.
DR   PIR; JN0419; JN0419.
DR   AlphaFoldDB; P19175; -.
DR   SMR; P19175; -.
DR   STRING; 1240350.AMZE01000092_gene671; -.
DR   PRIDE; P19175; -.
DR   eggNOG; COG0085; Bacteria.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 2.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 2.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase.
FT   CHAIN           1..1357
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000047942"
FT   CONFLICT        1180
FT                   /note="T -> N (in Ref. 2; AAA25986)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1184
FT                   /note="I -> V (in Ref. 2; AAA25986)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1236
FT                   /note="F -> S (in Ref. 2; AAA25986)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1357 AA;  151306 MW;  4AEDB68C8B086EA6 CRC64;
     MAYSYTEKKR IRKDFSKLPD VMDVPYLLAI QLDSYREFLQ AGSIQGSLPR RRLHAAFKSV
     FPIISYSGNA ALEYVGYRLG EPAFDVKECV LRGVTFAVPL RVKVRLIIFD KESSNKAIKD
     IKEQEVYMGE IPLMTENGTF VINGTERVIV SQMHRSPGVF FDHDRGKTHS SGKLLYSARI
     IPYRGSWLDF EFDPKDCVFV RIDRRRKLPA SVLLRALGYS TEEVLNTFYT TNVFHISGEK
     LSLELVPQRL AGEVAVMDIH DETGKVIVEQ GRRITARHIN QLEKAGVKQL DVPMEYVLGR
     TTAKAIVHPA TGEILAECNT EMTTELLIKV AKAQVVRIET LYTNDIDCGP FISDTLKIDT
     TSNQLEALVE IYRMMRPGEP PTKDAAETLF NNLFFSAERY DLSPLGRMKF NRRIGRTEIE
     GSGVLSKEDI VEVLKTLVDI RNGKGIVDDI DHLGNRRVRC VGEMAENQFR VGLVRVERAV
     KERLSMAESE GLMPQDLINA KPVAAAVKEF FGSSQLSQFM DQNNPLSEIT HKRRCSALGP
     GGLTRERAGF EVRDVHPTHY GRVCPIETPE GPNIGLINSL AAYARTNQYG FLESPYRVVK
     EGVVSDDIVF LSAIEEADHV IAQASAAMND KKQLIDELVA VRHLNEFTVK APEDVTLMDV
     SPKQVVSVAA SLIPFLEHDD ANRALMGSNM QRQAVPTLRA DKPLVGTGME RNVARDSGVC
     VVRRRGGVID SVDASRIVVR VADDEVETGE RRVDIYNLTK YTRSNQNTCI NQRPLVSKGD
     KVQRGDIMAD RASTDMGELA LGQNMRIAFM AWNGFNFEDS ICLSERVVQE DRFTTIHIQE
     LTCVARDNKL GPREITSGIP NVGEAALNKL DEAGIVYVGA EVGAGDILVG KVTPKGHTQL
     TPEEKLLRAI FGEKASDVKD TSLRVPTGTQ GTVIDVQVFT RDGVERDSRA LAIEKMQLDE
     IPQDLNEEFR IVEGATFERL RSALNGQVVD GGAGLKKGTV ITDEVLDGLD DGQWFKLRMA
     EDALNEQLEK AQQYIVDRRR LLDDKFEDKK RNVQQGDDLA PGVLKIVKVY LAIRRRIQPG
     DKMAGRHGNK GVVSVIMPVE DMPHDANGTP VDVVLNPLGV PSRMNVGQIL ETHLGLAAKG
     LGEKIDRMLE EQRKAAELRV FLTEVYNEIG GRQENLDEFT DEEILALANN LKKGVPMATP
     VFDGAKEREI KAMLKLADLP ESGQMVLFDG RTRNKFERPV TVGYMYMLKL NHLVDDKMHA
     RSTGSYSLVT QQPLGGKAQF GGQRFGEMEV WALEAYGAAY TLQEMLTVKS DDVNGRTKMY
     KNIVDGDHRM EPGMAESFNV LIKEIRSLGI DIDLETE