RPOB_PSEU5
ID RPOB_PSEU5 Reviewed; 1356 AA.
AC A4VHM3;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 1.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=PST_0777;
OS Pseudomonas stutzeri (strain A1501).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=379731;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=A1501;
RX PubMed=18495935; DOI=10.1073/pnas.0801093105;
RA Yan Y., Yang J., Dou Y., Chen M., Ping S., Peng J., Lu W., Zhang W.,
RA Yao Z., Li H., Liu W., He S., Geng L., Zhang X., Yang F., Yu H., Zhan Y.,
RA Li D., Lin Z., Wang Y., Elmerich C., Lin M., Jin Q.;
RT "Nitrogen fixation island and rhizosphere competence traits in the genome
RT of root-associated Pseudomonas stutzeri A1501.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:7564-7569(2008).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000304; ABP78474.1; -; Genomic_DNA.
DR RefSeq; WP_011911981.1; NC_009434.1.
DR AlphaFoldDB; A4VHM3; -.
DR SMR; A4VHM3; -.
DR STRING; 379731.PST_0777; -.
DR PRIDE; A4VHM3; -.
DR EnsemblBacteria; ABP78474; ABP78474; PST_0777.
DR GeneID; 66819919; -.
DR KEGG; psa:PST_0777; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_6; -.
DR OMA; FMTWEGY; -.
DR Proteomes; UP000000233; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1356
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300377"
SQ SEQUENCE 1356 AA; 150971 MW; 2076EF9DF66BC75F CRC64;
MAYSYTEKKR IRKDFSKLPH VMDVPYLLAI QLDSYREFLQ AGATKDQFRD IGLHAAFKSV
FPIISYSGNA ALEYVGYRLG EPAFDVKECV LRGVTFAVPL RVKVRLIIFD KESSNKAIKD
IKEQEVYMGE IPLMTENGTF IINGTERVIV SQLHRSPGVF FDHDRGKTHS SGKLLYSARI
IPYRGSWLDF EFDPKDAVFV RIDRRRKLPA SVLLRALNYS TEEILDAFYD TNVFHVKGET
LALELVPQRL RGEIATFDIK DDSGKVIVEQ GRRITARHIN QLDKSGIKEL EMPMDYVLGR
TVAKAIVHPA TGEIIAECNT ELTVDVMAKI VKAQVVRFET LYTNDIDCGP FISDTLKIDS
TTNQLEALVE IYRMMRPGEP PTKDAAETLF NNLFFAAERY DLSAVGRMKF NRRIGRTEIE
GSGVLSREDI VAVLKTLVDI RNGKGIVDDI DHLGNRRVRC VGEMAENQFR VGLVRVERAV
KERLSMAESE GLMPQDLINA KPVAAAVKEF FGSSQLSQFM DQNNPLSEIT HKRRVSALGP
GGLTRERAGF EVRDVHPTHY GRVCPIETPE GPNIGLINSL AAYARTNQYG FLESPYRVVK
DGEVTDEIVF LSAIEEADHV IAQASAKLDG RKLVDELVAV RHLNEFTVKA PEDVTLMDVS
PKQVVSVAAS LIPFLEHDDA NRALMGSNMQ RQAVPTLRSD KPLVGTGMER NVARDSGVCV
VARRGGVIDS VDASRIVVRV RDDEVETGEA GVDIYNLTKY TRSNQNTCIN QRPLVRKGDV
VARGDIMADG PSTDMGELAL GQNMRVAFMP WNGYNFEDSI LLSERVVQED RFTTIHIQEL
TCVSRDTKLG PEEITADIPN VGEAALNKLD EAGIVYVGAE VGPGDILVGK VTPKGETQLT
PEEKLLRAIF GEKASDVKDT SLRVPTGTKG TVIDVQVFIR DGVERDSRAL AIEKMQLDEI
RKDLNEEFRI VEGATFERLR SALVGATAEG GAGLKKGVVI TDEILDGLEH GQWFKLRMAE
DALNEQLEKA QAYLSDRRQL LDDKFEDKKR KLQQGDDLAP GVLKIVKVYL AIKRRIQPGD
KMAGRHGNKG VVSVIMPVED MPHDANGTPV DIVLNPLGVP SRMNVGQILE THLGLAAKGL
GEKINLMLEE QRKVAELRKF LHEIYNEIGG RQENLDDLSD QEVLDLANNL RKGVPMATPV
FDGAKEREIK AMLKLADLPE SGQMQLFDGR TGNAFERTTT VGYMYMLKLN HLVDDKMHAR
STGSYSLVTQ QPLGGKAQFG GQRFGEMEVW ALEAYGAAYT LQEMLTVKSD DVNGRTKMYK
NIVDGDHRME AGMPESFNVL IKEIRSLGID IDLETE
