ID   RPOB_PSYA2              Reviewed;        1372 AA.
AC   Q4FQH3;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   30-AUG-2005, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Psyc_1887;
OS   Psychrobacter arcticus (strain DSM 17307 / VKM B-2377 / 273-4).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Moraxellales; Moraxellaceae;
OC   Psychrobacter.
OX   NCBI_TaxID=259536;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 17307 / VKM B-2377 / 273-4;
RX   PubMed=20154119; DOI=10.1128/aem.02101-09;
RA   Ayala-del-Rio H.L., Chain P.S., Grzymski J.J., Ponder M.A., Ivanova N.,
RA   Bergholz P.W., Di Bartolo G., Hauser L., Land M., Bakermans C.,
RA   Rodrigues D., Klappenbach J., Zarka D., Larimer F., Richardson P.,
RA   Murray A., Thomashow M., Tiedje J.M.;
RT   "The genome sequence of Psychrobacter arcticus 273-4, a psychroactive
RT   Siberian permafrost bacterium, reveals mechanisms for adaptation to low-
RT   temperature growth.";
RL   Appl. Environ. Microbiol. 76:2304-2312(2010).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000082; AAZ19735.1; -; Genomic_DNA.
DR   RefSeq; WP_011281145.1; NC_007204.1.
DR   AlphaFoldDB; Q4FQH3; -.
DR   SMR; Q4FQH3; -.
DR   STRING; 259536.Psyc_1887; -.
DR   PRIDE; Q4FQH3; -.
DR   EnsemblBacteria; AAZ19735; AAZ19735; Psyc_1887.
DR   KEGG; par:Psyc_1887; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_3_6; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000000546; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1372
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000224098"
SQ   SEQUENCE   1372 AA;  152758 MW;  97CDB72972EA6201 CRC64;
     MAYSYTEKKR IRKSFAELPT VMDIPYLLSI QVDSYEQFLQ EHKKPKAREN TGLQAAYSSI
     FPIESHSGNA ELQFVEYYLG TPEFDERECI LRGSTFAAPM RVKIRLIIKD KDSKDKDSKA
     AIKDIREQSV YMGEMPLMTA NGTFIINGTE RVIVSQLHRS PGVFFDHDKG KSHSSGKVLY
     NARIIPYRGS WLDFEFDAKD LVFARIDRRR KLLASIILRA LGLSTSEILD LFFDKVKVYK
     GEEQFEIDLV ADRLRGEMAQ FDIVTPEGDV VVEQGKRINA RRIRQLEEAG MTKISIPDEY
     LYERILAEDI IVNDEVIARA NTLIDHELLV KLSAFEASES IKEFSILFTN DIDQGSYIAD
     TLRADSTSSR EEALIEIYKV MRPGEPPTVE TAEKLFDSMF FNADRYDLSN VGRMKFNRRL
     GLDFVDTDDA DIQRERSVLT NADIVNVLKE LIEIRNGRGE VDDIDHLGNR RIRSVGEMAE
     NQFRVGLVRV ERAVKERLSS AESDNLSPQD LINSKPVAAA VKEFFGSSQL SQFMDQNNPL
     SEVTHKRRVS ALGPGGLTRE RAGFEVRDVH DTHYGRVCPI ETPEGPNIGL INSLATFAKT
     NSFGFLETPY RRVVDGKVTD VIEYLSAIEE VGTVIAQADS PVTADGALSD EMVSVRSYGE
     FVRMPPEKVT HMDVSPSQVV SVAAGLIPFL EHDDANRALM GSNMQRQAVP TLRADKPLVG
     TGMERHVARD SGVCVIAKRG GVIEDVDASR IVVRVNEAEM IAGEAGIDIY NLVKYTRSNQ
     NTCINQRIIV NQGDEIAFGD ILADGPSTDL GELALGQNIR IAFMPWNGYN FEDSILLSEK
     VVKEDRFTTI HIQELTCVAR DTKLGTEEIT ADIPNVGEAA LSSLDEAGIV YIGAEVDAGD
     ILVGKVTPKG ETQLTPEEKL LRAIFGEKAA DVKDTSLRVP TSSKGTVIDV QVFTRDGVEK
     DARARAIEKS QLDSYRKDLK EELRIFEEAA RGRIGNLLDG QKVSGGSGLK AGTIMALADM
     KDMSLETLLD IQPVEEEISE RLTQIAEYLV DKQKDIDVKF AEKKRKLTAG DDLQHGVQKI
     VKVYLAVKRR IQPGDKMAGR HGNKGVVSRI MPVEDMPYDE HGNTVDIVLN PLGVPSRMNI
     GQVLETHLGM AAKGLGEKID GMLKSQAAIK DLREFLDKIY NQVGGEQVDL DSLTDDDIMA
     LADNLRAGVP MGTAVFDGAK ESQVKDLLEL AGMDRDGQQT LYDGRTGQKF DRKVTVGYMY
     MLKLNHLVDD KMHARSTGSY SLVTQQPLGG KAQFGGQRFG EMEVWALEAY GATYTLQEML
     TVKSDDVEGR TRMYKNIVDG EQYMDPGMPE SFNVLTKEIK SLGINIELKQ TH