RPOB_PSYCK
ID RPOB_PSYCK Reviewed; 1372 AA.
AC Q1Q8P9;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Pcryo_2177;
OS Psychrobacter cryohalolentis (strain ATCC BAA-1226 / DSM 17306 / VKM B-2378
OS / K5).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Moraxellales; Moraxellaceae;
OC Psychrobacter.
OX NCBI_TaxID=335284;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-1226 / DSM 17306 / VKM B-2378 / K5;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D.,
RA Han C., Tapia R., Sims D.R., Gilna P., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Kim E., Richardson P.;
RT "Complete sequence of chromosome of Psychrobacter cryohalolentis K5.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000323; ABE75954.1; -; Genomic_DNA.
DR RefSeq; WP_011514490.1; NC_007969.1.
DR AlphaFoldDB; Q1Q8P9; -.
DR SMR; Q1Q8P9; -.
DR STRING; 335284.Pcryo_2177; -.
DR PRIDE; Q1Q8P9; -.
DR KEGG; pcr:Pcryo_2177; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_3_6; -.
DR OMA; FMTWEGY; -.
DR Proteomes; UP000002425; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1372
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300378"
SQ SEQUENCE 1372 AA; 152577 MW; 6D9BA3FCE764729B CRC64;
MAYSYTEKKR IRKSFAELPT VMDIPYLLSI QVDSYEQFLQ EHKKPKAREN TGLQAAYSSI
FPIESHSGNA ELQFVEYYLG TPEFDERECI LRGSTFAAPM RVKIRLIIKD KDSKDKDSKA
AIKDIREQSV YMGEMPLMTA NGTFIINGTE RVIVSQLHRS PGVFFDHDKG KSHSSGKVLY
NARIIPYRGS WLDFEFDAKD LVFARIDRRR KLLASIILRA LGLSTSEILD LFFDKVRVYK
GEEQFEIDLV ADRLRGEMAQ FDIVTPAGDV VVEQGKRINA RRIRQLEEAG MTKISIPDEY
LYERILAEDI IVNDEVIARA NTLIDHELLV KLSAFEASES IKEFSILFTN DIDQGSYIAD
TLRADSTSSR EEALIEIYKV MRPGEPPTVE TAEKLFDSMF FNADRYDLSN VGRMKFNRRL
GLDFVDTDDA DIQRERSVLT NADIVNVLKE LIEIRNGRGE VDDIDHLGNR RIRSVGEMAE
NQFRVGLVRV ERAVKERLSS AESDNLSPQD LINSKPVAAA VKEFFGSSQL SQFMDQNNPL
SEVTHKRRVS ALGPGGLTRE RAGFEVRDVH DTHYGRVCPI ETPEGPNIGL INSLATFAKT
NSFGFLETPY RRVVDGKVTD VIEYLSAIEE VGTVIAQADS PVTADGALSD EMVSVRSYGE
FVRMPPEKVT HMDVSPSQVV SVAAGLIPFL EHDDANRALM GSNMQRQAVP TLRADKPLVG
TGMERHVARD SGVCVIAKRG GVIEDVDASR VVVRVNEDEM IAGEAGIDIY NLVKYTRSNQ
NTCINQRIIV NQGDAIAVGD ILADGPSTDL GELALGQNIR IAFMPWNGYN FEDSILLSEK
VVKEDRFTTI HIQELTCVAR DTKLGTEEIT ADIPNVGEAA LSSLDEAGIV YIGAEVDAGD
ILVGKVTPKG ETQLTPEEKL LRAIFGEKAA DVKDTSLRVP TSSKGTVIDV QVFTRDGVEK
DARARAIEKS QLDSYRKDLK EELRIFEEAA RGRIGNLLDG QKVSGGSGLK AGTVMALADM
KDMSLETLLD IQPVEEEISE RLTQIAEYLV DKQKDIDVKF AEKKRKLTAG DDLQHGVQKI
VKVYLAVKRR IQPGDKMAGR HGNKGVVSRI MPVEDMPYDE NGNTVDIVLN PLGVPSRMNI
GQVLETHLGM AAKGLGEKID GMLKSQAAIK DLRDFLDKIY NQVGGEQVDL DSLSDDDIMA
LADNLRAGVP MGTAVFDGAR ESQVKDLLEL AGMDRDGQQT LYDGRTGQKF DRKVTVGYMY
MLKLNHLVDD KMHARSTGSY SLVTQQPLGG KAQFGGQRFG EMEVWALEAY GATYTLQEML
TVKSDDVEGR TRMYKNIVDG EQYMDPGMPE SFNVLTKEIK SLGINIELKQ SN
