RPOB_RALSO
ID RPOB_RALSO Reviewed; 1368 AA.
AC Q8XUZ8;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=RSc3034;
GN ORFNames=RS04723;
OS Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Ralstonia.
OX NCBI_TaxID=267608;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GMI1000;
RX PubMed=11823852; DOI=10.1038/415497a;
RA Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M.,
RA Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M.,
RA Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M.,
RA Moisan A., Robert C., Saurin W., Schiex T., Siguier P., Thebault P.,
RA Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A.;
RT "Genome sequence of the plant pathogen Ralstonia solanacearum.";
RL Nature 415:497-502(2002).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; AL646052; CAD16743.1; -; Genomic_DNA.
DR RefSeq; WP_011002929.1; NC_003295.1.
DR AlphaFoldDB; Q8XUZ8; -.
DR SMR; Q8XUZ8; -.
DR STRING; 267608.RSc3034; -.
DR PRIDE; Q8XUZ8; -.
DR EnsemblBacteria; CAD16743; CAD16743; RSc3034.
DR GeneID; 60502541; -.
DR KEGG; rso:RSc3034; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_3_4; -.
DR OMA; FMTWEGY; -.
DR Proteomes; UP000001436; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1368
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000047944"
SQ SEQUENCE 1368 AA; 152428 MW; 7116CF08954C73E7 CRC64;
MAYSFTEKKR IRKSFAKRAT VHQVPFLLAT QIQSYAQFLQ EHASVAQRKS EGLQAAFNAI
FPIVSHNGLA RMEFVSYHLS NPPFDVKECQ QRGLTFHSAL RAKVRLIIND RENPTKVKEI
KEQEVYMGEI PLMTSTGSFV INGTERVIVS QLHRSPGVFF EHDKGKTHSS GKLLFSARII
PYRGSWLDFE FDPKDILYFR VDRRRKMPVT ILLKSIGLTP EQILAHFFVF DNFTLKSDGA
LMEFVPERLR GEVARFDISD KNGKVVVEKD KRINAKHIRD LDAAGTKLIS VPEDYLLGRV
LAKNIVDPDT GEVLANANDE LTEGVLEKLR DAGVKEIQTL YTNDLDQGPY MSQTLRTDDT
VDQTAARIAI YRMMRPGEPP TEDAVEALFQ RLFYSEDSYD LSRVGRMKVN SRLNRSEGTG
PMVLTDDDIL DTIKLLVNLR NGKGEVDDID HLGNRRVRCV GELAENQFRA GLSRVERAVK
ERLGQAETEN LMPHDLINSK PISSAIREFF GSSQLSQFMD QTNPLSEVTH KRRISALGPG
GLTRERAGFE VRDVHPTHYG RVCPIETPEG PNIGLINSLA LYAQLNDYGF LETPYRKVEN
SKLTDQVDYL SAIEEGKYVV AQANATVDKD GNLVDELVSA REGSERETRM VTPDRVQYID
VAPSQIVSAA ASLVPFLEHD DANRALMGAN MQRQAVPCLR ADKPLVGTGV ERTVAVDSGT
AVQATRGGLV DYVDANRVVI RVNDDEAVAG EVGVDIYNLI KYTRSNQNTN INQRPMVKVG
DIVARGDVIA DGASTDLGEL ALGQNMLVAF MPWNGYNFED SILISERVVA EDRYTSIHIE
ELSVVARDTK LGPEEITRDI SNLAEAQLAR LDESGITYIG AEVEAGDVMV GKVTPKGETQ
LTPEEKLLRA IFGEKASDVK DTSLRVPSGM SGTVIDVQVF TREGVTRDKR AQSIIDEELK
RYRLDLNDQL RIVEGDAFQR LERLLVGKVV NGGPKKLAKG TALTKEYLAD LDKWHWFDIR
PSDDDVATQL EAVKEAIEQK RHDFDLAFEE KRKKLTQGDE LPPGVIKMVK VYLAVKRRLQ
PGDKMAGRHG NKGVVSKITP IEDMPYMADG TPADIVLNPL GVPSRMNVGQ ILETHLGWAA
RGLGERIGNM LKAQAKAAEM RKLLGQIYNE SGKVEDLDSL SDAEVLELAE NLKKGVPFAT
PVFDGAHEDE IRRMLDLAYP EDIAKEKGLT ASKQQVTLFD GRTGEAFERP VTLGVMHMLK
LHHLVDDKMH ARSTGPYSLV TQQPLGGKAQ FGGQRFGEME VWALEAYGAS YVLQEMLTVK
SDDVNGRTKV YENIVKGEHS IDAGMPESFN VLVKEIRSLG IDIDLERN
