ID   RPOB_RHDSA              Reviewed;        1097 AA.
AC   A6MVX4;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 1.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS   Rhodomonas salina (Cryptomonas salina).
OG   Plastid; Chloroplast.
OC   Eukaryota; Cryptophyceae; Pyrenomonadales; Pyrenomonadaceae; Rhodomonas.
OX   NCBI_TaxID=52970;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CCMP1319 / NEPCC76 / CS-174;
RX   PubMed=17522086; DOI=10.1093/molbev/msm101;
RA   Khan H., Parks N., Kozera C., Curtis B.A., Parsons B.J., Bowman S.,
RA   Archibald J.M.;
RT   "Plastid genome sequence of the cryptophyte alga Rhodomonas salina
RT   CCMP1319: lateral transfer of putative DNA replication machinery and a test
RT   of chromist plastid phylogeny.";
RL   Mol. Biol. Evol. 24:1832-1842(2007).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; EF508371; ABO70811.1; -; Genomic_DNA.
DR   RefSeq; YP_001293553.1; NC_009573.1.
DR   AlphaFoldDB; A6MVX4; -.
DR   SMR; A6MVX4; -.
DR   GeneID; 5228507; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1097
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000300457"
SQ   SEQUENCE   1097 AA;  123025 MW;  2B235B393DC8647F CRC64;
     MSSTKLSTSI LPDLVEIQRA SFCWFLEEGL AEEIKSFSPI TDYTGNLELH FFGDQFKLKC
     PKYNLLESKR RDATYSVQVY VPARLINRDT GIIKEQEVFI GDLPLMTDRG TFIINGAERV
     IVNQIVRSPG IYYKSETDRQ GRRTYSGSLI SNRGAWVKFE TDRNDLVWVR IDKTRKIPAH
     VFLKAMGLSD SDIYNGLRHP EYLKKSFRVE GNYTTEEALI QMYTKLRPGE PATVNGGQQI
     LYSRFFDPKR YDLGKVGRYK INKKLALSIP ENIKVLTPQD TLSAIDYLIN LKFNIGETDD
     IDHLGNRRVR SVGELLQNQV RVGLNRLERI IRERMTICDS ESLAPNTLVN PKPIIAAIRE
     FFGSSQLSQF MDQTNPLAEL THKRRISALG PGGLNRDRAG FAVRDIHPSH YGRICPIETP
     EGPNAGLIGV LATHARINSY GFIETPFYQV INGKVVSDGN PVYLTADQED NFRIAPGDIA
     IDENNAINND IVPVRYRQEF TITKPEQIDY IQVSPIQVIS IATSLIPFLE HDDANRALMG
     SNMQRQAVPL LYPESPLIGT GIEAQAARDS GMVVVSYQDG RVTYVSANKI CITDDEGKEV
     VYYLQKYQRS NQDTCINQRP SVWLGEKVVA GQVIADGAAT EGGELALGQN ILIAYLPWEG
     YNYEDAFLIS ERLVYNDVYT SVHIEKYEIE ARQTKLGSEE ITRELPNIGE YSLRKLDDNG
     IIVIGSWVEV GDILVGKVTP KGESDQPPEG KLLRAIFGEK ARDVRDTSLR VPNGGRGRVL
     DVRIFTREKG DELPTGANIV IRVYVAQTRK IQVGDKMAGR HGNKGIISRI LPRQDMPYLP
     DGTPVDLVLN PLGVPSRMNV GQIFECLLGL AAENLDKRFK IIPFDEMNGA EASRVLVNEK
     LMEARTLTEK DWIFDLRHPG KTQLFDGRTG EAFDNPVTVG ISYMLKLVHL VDDKIHARST
     GPYSLVTQQP LGGKAQHGGQ RLGEMEVWAL EAFGASYTLQ ELLTVKSDDM QGRNETLNAI
     VKGKPIPRPG TPESFKVLMR ELQSLGLDIG AYKIENLPDG QTRGIEVDLM SNLHNRRVPS
     RPTYESITRE DLENSFA