RPOB_RHILO
ID RPOB_RHILO Reviewed; 1378 AA.
AC Q98N66;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=mlr0276;
OS Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)
OS (Mesorhizobium loti (strain MAFF 303099)).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Phyllobacteriaceae; Mesorhizobium.
OX NCBI_TaxID=266835;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LMG 29417 / CECT 9101 / MAFF 303099;
RX PubMed=11214968; DOI=10.1093/dnares/7.6.331;
RA Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S.,
RA Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y.,
RA Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y.,
RA Nakayama S., Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M.,
RA Tabata S.;
RT "Complete genome structure of the nitrogen-fixing symbiotic bacterium
RT Mesorhizobium loti.";
RL DNA Res. 7:331-338(2000).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; BA000012; BAB47896.1; -; Genomic_DNA.
DR RefSeq; WP_010909266.1; NC_002678.2.
DR AlphaFoldDB; Q98N66; -.
DR SMR; Q98N66; -.
DR STRING; 266835.14021283; -.
DR PRIDE; Q98N66; -.
DR EnsemblBacteria; BAB47896; BAB47896; BAB47896.
DR GeneID; 66684227; -.
DR KEGG; mlo:mlr0276; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_5; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000000552; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 2.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1378
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000047945"
SQ SEQUENCE 1378 AA; 153901 MW; 093449B2F351CBA5 CRC64;
MAQTQTFNGR RRVRKFFGKI PEVAEMPNLI EVQKASYDQF LMVDEPKGGR PDEGLQAVFK
SVFPISDFSG SSMLEFVKYE FEGPKFDVDE CRQRDLTYAA PLKVTLRLIV FDIDEDTGAK
SIKDIKEQDV YMGDMPLMTL NGTFIVNGTE RVIVSQMHRS PGVFFDHDKG KSHSSGKLLF
AARVIPYRGS WLDIEFDSKD VVHARIDRRR KIPVTSLLMA LGMDGEEILS TFYNKITYVR
AGDHWRIPFN VERFRGLKAV GDLVDADTGE IVVEAGKKIT ARQARQLGEK GLKAIKATDE
DLLGNYLAED IVNYATGEIF LEAGDEIDEK TLKVLLGTGE QEIKVLDIDH VNVGAYIRNT
LNVDKNESRQ DALFDIYRVM RPGEPPTLET AEAMFNSLFF DSERYDLSAV GRVKMNMRLE
LKAEDTVRVL RKEDILAVVK TLVELRDGKG EIDDIDNLGN RRVRSVGELM ENQYRVGLLR
MERAIKERMS SIEIDTVMPQ DLINAKPAAA AVREFFGSSQ LSQFMDQTNP LSEITHKRRL
SALGPGGLTR ERAGFEVRDV HPTHYGRICP IETPEGPNIG LINSLATFAR VNKYGFIESP
YRKIVDGKLT NDVVYLSAME EAKHHVAQAN AELDKNGGFV DEFVICRSAG EVMMAPRENV
DLMDVSPKQM VSVAAALIPF LENDDANRAL MGSNMQRQAV PLVRAEAPFV GTGMEPIVAR
DSGAAIGARR GGIVDQVDAT RIVIRATEDL DPGKSGVDIY RLMKFQRSNQ NTCINQRPLV
RMGDRVNKGD IIADGPSTEL GDLALGRNVL VAFMPWNGYN YEDSILLSER IVADDVFTSI
HIEEFEVMAR DTKLGPEEIT RDIPNVSEEA LKNLDEAGIV YIGAEVQPGD ILVGKITPKG
ESPMTPEEKL LRAIFGEKAS DVRDTSMRMP PGTFGTVVEV RVFNRHGVEK DERAMAIERE
EIERLAKDRD DEQAILDRNV YARLSDVLVG KEAIAGPKGF KKGSTLSKDT LDEYPRSQWW
QFAVENEKLQ SELEALRGQY DDSKKALEQR FMDKVEKVQR GDEMPPGVMK MVKVFVAVKR
KMQPGDKMAG RHGNKGVVSR IVPVEDMPFL EDGTHADIVL NPLGVPSRMN VGQILETHLG
WACAGMGKKI GELIDTYKAA GDIKPLRKTL ESFMPANDRN EPIREYDDES IVRLSEQMRR
GVSIATPVFD GAHEADINIM LEQAGLHTSG QSQLYDGRTG EPFDRKVTMG YIYMLKLHHL
VDDKIHARSI GPYSLVTQQP LGGKAQFGGQ RFGEMEVWAL EAYGAAYTLQ EMLTVKSDDV
AGRTKVYEAI VRGDDTFEAG IPESFNVLVK EMRSLGLNVE LENTKLDDAP VRLPDAAE