ID   RPOB_RHIME              Reviewed;        1380 AA.
AC   Q92QH7;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=R01348;
GN   ORFNames=SMc01317;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481430; DOI=10.1073/pnas.161294398;
RA   Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA   Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA   Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA   Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA   Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT   "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT   meliloti strain 1021.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AL591688; CAC45927.1; -; Genomic_DNA.
DR   RefSeq; NP_385454.1; NC_003047.1.
DR   RefSeq; WP_003536195.1; NC_003047.1.
DR   AlphaFoldDB; Q92QH7; -.
DR   SMR; Q92QH7; -.
DR   STRING; 266834.SMc01317; -.
DR   EnsemblBacteria; CAC45927; CAC45927; SMc01317.
DR   GeneID; 61602810; -.
DR   KEGG; sme:SMc01317; -.
DR   PATRIC; fig|266834.11.peg.2763; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_0_5; -.
DR   OMA; FMTWEGY; -.
DR   Proteomes; UP000001976; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 2.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 2.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1380
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000047946"
SQ   SEQUENCE   1380 AA;  153518 MW;  D44C334D148D5715 CRC64;
     MAQTLSFNGR RRVRKFFGKI PEVAEMPNLI EVQKASYDQF LMVDEPQGGR PDEGLQAVFK
     SVFPIKDFSG ASMLEFVSYE FEAPKFDVEE CRQRDLTYAA PLKVTLRLIV FDIDEDTGAK
     SIKDIKEQNV YMGDMPLMTD NGTFIVNGTE RVIVSQMHRS PGVFFDHDKG KSHSSGKLLF
     AARVIPYRGS WLDIEFDAKD IVHARIDRRR KIPVTSLLMA LGMDGEEILD TFYTKSLYQR
     DGEGWRVPFQ PDALKGQKTL ADMIDADTGE VVVESGKKLT PRLLRQLQEK GLKALKATDD
     DLYGNYLAED VVNFETGEIY LEAGDEIDEK TLPVILSAGF DEIPVLDIDH INIGAYIRNT
     LSADKNENRQ DALFDIYRVM RPGEPPTMDS AEAMFNALFF DAERYDLSAV GRVKMNMRLD
     LDVPDTVRTL RKEDILAVVK MLVELRDGKG EIDDIDNLGN RRVRSVGELM ENQYRLGLLR
     MERAIKERMS SIEIDTVMPQ DLINAKPAAA AVREFFGSSQ LSQFMDQVNP LSEITHKRRL
     SALGPGGLTR ERAGFEVRDV HPTHYGRICP IETPEGPNIG LINSLATFAR VNKYGFIESP
     YRKIVDGKVT NDVVYLSAME EAKYHVAQAN SVLDDDGSFS EEFVVCRHAG EVMLAPRDNI
     NLMDVSPKQL VSVAAALIPF LENDDANRAL MGSNMQRQAV PLLRAEAPFV GTGMEPVVAR
     DSGAAIAARR GGIVDQVDAT RIVIRATEDL DPSKSGVDIY RLQKFQRSNQ NTCVNQRPLV
     TVGDVINKGD IIADGPSTDL GDLALGRNAL VAFMPWNGYN YEDSILLSER IVRDDVFTSI
     HIEEFEVMAR DTKLGPEEIT RDIPNVSEEA LKNLDEAGIV YIGAEVQPGD ILVGKITPKG
     ESPMTPEEKL LRAIFGEKAS DVRDTSMRMP PGTFGTVVEV RVFNRHGVEK DERAMAIERE
     EIERLAKDRD DEQAILDRNV YARLVDMLRG HVAVAGPKGF KKGTELSNVV ISEYPRSQWW
     MFAIEDEKAQ GEIEALRAQY DESKSRLEQR FMDKVEKVQR GDEMPPGVMK MVKVFVAVKR
     KIQPGDKMAG RHGNKGVVSR IVPIEDMPFL EDGTHVDVVL NPLGVPSRMN VGQILETHLG
     WACAGMGKKI GAMLDAYKAG ADIQPLRDTI DSVIGSGPKG EPIKQYDDES IVRLAEQTRR
     GVSIATPVFD GAVEADVNEM LEQAGLKVTG QSTLYDGRTG ETFDRQVTVG YIYMLKLNHL
     VDDKIHARSI GPYSLVTQQP LGGKAQFGGQ RFGEMEVWAL EAYGAAYTLQ EMLTVKSDDV
     AGRTKVYEAI VRGDDTFEAG IPESFNVLVK EMRSLGLSVE LENSKVDDVG STAQLPDAAE