ID   RPOB_RHIWR              Reviewed;        1455 AA.
AC   A5VBZ8;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Swit_3468;
OS   Rhizorhabdus wittichii (strain DSM 6014 / CCUG 31198 / JCM 15750 / NBRC
OS   105917 / EY 4224 / RW1) (Sphingomonas wittichii).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Rhizorhabdus.
OX   NCBI_TaxID=392499;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 6014 / CCUG 31198 / JCM 15750 / NBRC 105917 / EY 4224 / RW1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C.,
RA   Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M., Hauser L.,
RA   Kyrpides N., Kim E., Halden R.U., Miller T.R., Salzberg S.L., Eisen J.A.,
RA   Richardson P.;
RT   "Complete sequence of chromosome of Sphingomonas wittichii RW1.";
RL   Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000699; ABQ69814.1; -; Genomic_DNA.
DR   RefSeq; WP_012049655.1; NC_009511.1.
DR   AlphaFoldDB; A5VBZ8; -.
DR   SMR; A5VBZ8; -.
DR   STRING; 392499.Swit_3468; -.
DR   EnsemblBacteria; ABQ69814; ABQ69814; Swit_3468.
DR   KEGG; swi:Swit_3468; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_0_5; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000001989; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1455
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000329189"
SQ   SEQUENCE   1455 AA;  160894 MW;  DD64F81B86889FEB CRC64;
     MATQAVPATA KKRIRKVFGN IHEVVQMPNL IEVQRESYEQ FLRSRPADGY VSGLEKTLRS
     VFPIRDFAGT AELDFVQYEL EDPKYDTDEC RQRGMTYAAP MRVTLRLIVF EVDPDTETRS
     VLDIKEQDVY MGDMPLMTEN GTFLINGTER VIVSQMHRSP GVLFDHDRGK THASGKYLFA
     ARVIPYRGSW LDFEFDAKDI VNVRIDRKRK LPVTSLLRAL GDAVIEVNPD KGKFEAGDIV
     AISGVNRACR VKAVAGRLVT VEDPTGAIIP GGLIQTAEGE KIGHVARIRL QGMSGEDILN
     YFYRTQRFVR GENGWKVPFV AENWRGQKPA FDVVNADTGE VVFPAGTKIS PRAANKAGKD
     GLETLLIPTD QIFGRYSAYD LIDESTGRIY IEAGDEVTPE NLEALDKAGF DHVDLLDIDH
     VSTGPWIRNT LKADKVEDRD HALSEIYRVM RPGEPPTKET AEALFAGLFF DSERYDLSAV
     GRVKLNMRLE LDAEDTVTTL RSEDILAVVK TLVDLKDGKG EIDDIDNLGN RRVRSVGELL
     ENQYRVGLLR MERAVKERMS SVDVSTAMPN DLINAKPAVA AVREFFGSSQ LSQFMDQTNP
     LSEVTHKRRV SALGPGGLTR ERAGFEVRDV HPTHYGRICP IETPEGPNIG LINSLASFSR
     VNKYGFIETP YRKIVDGKVT NEVVYLSAME EAKHTIAQAN AELDSEGRFV EDLISAREAG
     EFLMAPRDHV TLMDVSPKQL VSVAASLIPF LENDDANRAL MGSNMQRQAV PLVRAEAPFV
     GTGMEETVAR DSGAAIAAKR SGIVDQVDAT RIVVRATGAV DAGKSGVDIY TLMKFQRSNQ
     STCINQRPLV KVGDVVNAGD IIADGPSTEF GELALGRNVL VAFMPWNGYN YEDSILISER
     IVKDDVFTSI HIDEFEVMAR DTKLGPEDIT RDIPNVGEEA LRNLDEAGIV YIGAEVEPGD
     ILCGKITPKG ESPMTPEEKL LRAIFGEKAS DVRDTSLRLP PGVSGTVVDV RVFNRHGIDK
     DERAMAIERE EIDRLTKDRE DERAILNRAN YARLKEMLLG QIATAAPKGI KKGSEINEEL
     LAEVEKREWW KFAVQDDARQ ADLEAVKAQY DDAVGLIVKK FEDRVEKLQR GDELPPGVLK
     MVKVFVAVKR KLQPGDKMAG RHGNKGVISR ILPQEDMPFL EDGTPVDIVL NPLGVPSRMN
     VGQIFETHLG WAARGLGKQV TSALEAWREA NPDAQAATPP AAVVDRLKEV YGKEYHADIE
     GRSTDQIVEM AGLLKNGVPM ATPVFDGARE ADVAEMLRRA GLDESGQVEL FDGRTGDQFD
     RKVTVGYIYM LKLHHLVDDK IHARSIGPYS LVTQQPLGGK AQFGGQRFGE MEVWALQAYG
     AAYTLQEMLT VKSDDVVGRT KVYEAIVKGD DTFEAGIPES FNVLVKEMRS LGLNVELSSI
     EDQSDDDDGL AEAAE