RPOB_RHOBA
ID RPOB_RHOBA Reviewed; 1240 AA.
AC Q7URW6;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 16-JAN-2004, sequence version 2.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=RB5414;
OS Rhodopirellula baltica (strain DSM 10527 / NCIMB 13988 / SH1).
OC Bacteria; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae;
OC Rhodopirellula.
OX NCBI_TaxID=243090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 10527 / NCIMB 13988 / SH1;
RX PubMed=12835416; DOI=10.1073/pnas.1431443100;
RA Gloeckner F.O., Kube M., Bauer M., Teeling H., Lombardot T., Ludwig W.,
RA Gade D., Beck A., Borzym K., Heitmann K., Rabus R., Schlesner H., Amann R.,
RA Reinhardt R.;
RT "Complete genome sequence of the marine planctomycete Pirellula sp. strain
RT 1.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:8298-8303(2003).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAD74221.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BX294142; CAD74221.1; ALT_INIT; Genomic_DNA.
DR RefSeq; NP_866682.1; NC_005027.1.
DR RefSeq; WP_007340662.1; NC_005027.1.
DR AlphaFoldDB; Q7URW6; -.
DR SMR; Q7URW6; -.
DR STRING; 243090.RB5414; -.
DR PRIDE; Q7URW6; -.
DR EnsemblBacteria; CAD74221; CAD74221; RB5414.
DR KEGG; rba:RB5414; -.
DR PATRIC; fig|243090.15.peg.2600; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_0; -.
DR InParanoid; Q7URW6; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000001025; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1240
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000047947"
SQ SEQUENCE 1240 AA; 138007 MW; DC9285B1850B36CE CRC64;
MATTTQRRLE PTSIRKFGTG LGQFDLPDLT ALQTVSYAAF LQEDAASNAR KDHGLESVLR
EIFPIASYDG NTTLEYLYYE LGKPRYTIQE CRQLRLTYGR PLRIWLRLNR EEPIEEEVYL
GDLPIMLGGG EFIINGAERV VVSQLHRSPG VDFVLEQDTT TDRKLPSCRV IPERGSWIEV
NVTKKDALTV RIDQSGKFAA TMLLRAMDPK YSTDADLLSA FYETGTIKVN GGKDATKIEN
KIAVDDVVYP SGHERAGEII VEAGYKITTE LSETICNAGV TSVEAMDLPK VPLIFNTLAD
DNTASHEEAL LRIYQRLRPG NPPQLEKART LFQEKFYDDN RYRLGKVGRF RLNRKLGLGV
EETVMTLRPD DMIAAIRYLI DLFDADSGAE IDDIDHLGNR RLRTIDELAS EELRKGFLKL
RRTVQERMSV KDAQDMTPRS LINPKSVSAA IDFFFGRGEL SQVVDQTNPL SQLAHERRLS
ALGPGGLNRK RAGFEVRDVH ISHYGRICPI ETPEGTNIGL ISSLAIYAGV DDYGFLCTPF
RKVIDGKISD ETVWLRADEE GESYVAPADT EVKDGALVPG PNMIARFRSD FEIVMPEQVS
FMDVAPAQMV GVSAGLIPFL EHDDANRALM GSNMQRQAVP LLVTEPPIVG TGMEREVAKN
SAMVVRARRA GKVTYADATR IEIGSDHYPL KKYQGLNERT CQNQKPLITV GDKVEKGQII
ADGAATQKGE LALGRNVLVG FMSFDGFNYE DAIIISEELV RNDTYTSIHI EDFDVEIRET
KLGREEFTRD IPNVSEKALR NLDDTGIVQT GTYVKPGDIL VGKVSPKSKT ELTPEEKLLH
AIFGRAGEDV KNDSLEVPSG IEGIVIDTQK FSRRMSLGED ERKAFERELK QHETEGNEEI
ASTFESLIRD MEEASGVKLK DSTGTPLADG QDPKFVAERA TSFRLELVLE QIEDEEKRKA
AEKVHQTQWQ NVEQAIDERD RKLNSMKRGD ELRSGVLQMV KIYIATKRTI SVGDKMAGRH
GNKGVIAKIL PIEDMPFLPD GTPIQIMLNP LGVPSRMNVG QILETHLGWA GAKLGFQALT
PIFDGASESE INDCLAEAGL PAHGKIRLTD GRTGEPMEQE TTVGYIYMLK LHHLVDDKVH
ARSTGPYSLI TQQPLGGKAR FGGQRFGEME VWALEAYGAA YILQELLTVK SDDVEGRTKI
YESMVKGENT LEAGTPASFD VLTNEIRGLA LNMQLEKRPI
