RPOB_ROSDO
ID RPOB_ROSDO Reviewed; 1378 AA.
AC Q160X7;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2006, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=RD1_4019;
OS Roseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp.
OS (strain OCh 114)) (Roseobacter denitrificans).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Roseobacteraceae; Roseobacter.
OX NCBI_TaxID=375451;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33942 / OCh 114;
RX PubMed=17098896; DOI=10.1128/jb.01390-06;
RA Swingley W.D., Sadekar S., Mastrian S.D., Matthies H.J., Hao J., Ramos H.,
RA Acharya C.R., Conrad A.L., Taylor H.L., Dejesa L.C., Shah M.K.,
RA O'Huallachain M.E., Lince M.T., Blankenship R.E., Beatty J.T.,
RA Touchman J.W.;
RT "The complete genome sequence of Roseobacter denitrificans reveals a
RT mixotrophic rather than photosynthetic metabolism.";
RL J. Bacteriol. 189:683-690(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000362; ABG33466.1; -; Genomic_DNA.
DR RefSeq; WP_011570076.1; NZ_FOOO01000006.1.
DR AlphaFoldDB; Q160X7; -.
DR SMR; Q160X7; -.
DR STRING; 375451.RD1_4019; -.
DR PRIDE; Q160X7; -.
DR EnsemblBacteria; ABG33466; ABG33466; RD1_4019.
DR KEGG; rde:RD1_4019; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_5; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000007029; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1378
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300391"
SQ SEQUENCE 1378 AA; 153395 MW; FC9B7A8D87426F7D CRC64;
MAQSFLGQKR LRKYYGKIRE VLDMPNLIEV QKSSYDLFLN SGEAEVPTDG EGIAGVFQSV
FPIKDFNETS VLEYVKYELE KPKYDVEECQ QRDMTYSAPL KVTLRLIVFD VDEDTGAKSV
KDIKEQDVFM GDMPLMTPNG TFVVNGTERV IVSQMHRSPG VFFDHDKGKT HSSGKLLFAC
RIIPYRGSWL DFEFDAKDIV FARIDRRRKL PVTTLLYSLG LDQEAIMDAY YDTITYKLEK
NKGWVAPFFP DRVRGTRPTY DLVDAASGEV LFERGKKVTP RAVKKLIDEG KVTELLLPYE
HIAGKFVAKD IINEETGAIY VEAGDELTLE YDKDGTLIGG TAKELVDAGI TEIPVLDIDN
VNVGPYMRNT MAQDKNMNRD TALMDIYRVM RPGEPPTVEA ASALFDTLFF DAERYDLSAV
GRVKMNMRLA LDAEDTQRTL RREDIVACIK ALVDLRDGRG DIDDIDHLGN RRVRSVGELM
ENQYRVGLLR MERAIKERMS SVEIDTVMPQ DLINAKPAAA AVREFFGSSQ LSQFMDQTNP
LSEVTHKRRL SALGPGGLTR ERAGFEVRDV HPTHYGRMCP IETPEGPNIG LINSLATFAR
VNKYGFIETP YRKVENRMVT DEVQYMSATE EMRHTVAQAN AQLDEDGRFK NDLVSTRQSG
DYTLAPSENV DLIDVSPKQL VSVAASLIPF LENDDANRAL MGSNMQRQAV PLLQAEAPLV
GTGIEEVVAR DSGAAIMAKR AGIIDQVDAQ RIVIRATSDL ELGDAGVDIY RMRKFQRSNQ
NTCINQRPLV KVGDTVLKGQ VIADGPSTDM GELALGKNVV VAFMPWNGYN YEDSILISER
IARDDVFTSI HIEEFEVAAR DTKLGPEEIT RDIPNVGEEA LRNLDEAGIV YIGADVEPGD
ILVGKITPKG ESPMTPEEKL LRAIFGEKAS DVRDTSLRVK PGDFGTVVEV RVFNRHGVEK
DERALQIERE EVERLARDRD DEMAILDRNI YARLKSTILG KVAVKGPKGV SANAEITEDL
LQMLPRVQWW QLALKDEGDA QVVEALNEQY EIQKRTLDAR FEDKVEKVRR GDDLPPGVMK
MVKVFVAVKR KLQPGDKMAG RHGNKGVISK VVPMEDMPFL ADGTPVDFCL NPLGVPSRMN
VGQILETHMG WAARGLGLNV DEALQEYRRS GDLTPVRDAL SHAYGENVYD EGIAGMDEET
LIEAAGNVTR GVPIATPVFD GAKEGDVNDA LVRAGFSESG QSILFDGRTG EQFARPVTVG
VKYLLKLHHL VDDKIHARST GPYSLVTQQP LGGKAQFGGQ RFGEMEVWAL EAYGAAYTLQ
EMLTVKSDDV AGRTKVYESI VKGEDNFEAG VPESFNVLVK EVRGLGLNME LLDAEEDE
