ID   RPOB_RUEST              Reviewed;        1379 AA.
AC   Q1GK49;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   27-JUN-2006, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=TM1040_0234;
OS   Ruegeria sp. (strain TM1040) (Silicibacter sp.).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Roseobacteraceae; Ruegeria; unclassified Ruegeria.
OX   NCBI_TaxID=292414;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TM1040;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Brettin T., Bruce D., Han C., Tapia R., Goodwin L., Thompson L.S.,
RA   Gilna P., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.,
RA   Belas R., Moran M.A., Buchan A., Gonzalez J.M., Schell M.A., Sun F.,
RA   Richardson P.;
RT   "Complete sequence of chromosome of Silicibacter sp. TM1040.";
RL   Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000377; ABF62967.1; -; Genomic_DNA.
DR   RefSeq; WP_011537599.1; NC_008044.1.
DR   AlphaFoldDB; Q1GK49; -.
DR   SMR; Q1GK49; -.
DR   STRING; 292414.TM1040_0234; -.
DR   EnsemblBacteria; ABF62967; ABF62967; TM1040_0234.
DR   KEGG; sit:TM1040_0234; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_0_5; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000000636; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1379
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000300405"
SQ   SEQUENCE   1379 AA;  153833 MW;  BA7CA9880A2F4A69 CRC64;
     MAQTFLGQKR LRKYYGKIRE VLDMPNLIEV QKSSYDLFLR SGDAPQPLDG EGIKGVFQSV
     FPIKDFNETS VLEFVNYALE RPKYDVDECM QRDMTYSAPL KVTLRLIVFD VDEDTGAKSV
     KDIKEQDVFM GDMPLMTPNG TFIVNGTERV IVSQMHRSPG VFFDHDKGKT HSSGKLLFAC
     RIIPYRGSWL DFEFDAKDIV FARIDRRRKL PVTTLLYALG LDQEGIMDAY YNTVNFKLEK
     SRGWVTPFFP ERVRGTRPTY DLVDAATGEI ICEAGKKVTP RAVKKMIDEG NITELLVPFE
     HIVGKYVAKD IINEENGAIY VEAGDELTLE YDKDGDIIGG SVKELLDAGI TDIPVLDIDN
     VNVGPYMRNT MAQDKNMSRE TALMDIYRVM RPGEPPTVEA ASALFDTLFF DSERYDLSAV
     GRVKMNMRLA LDAEDTQRTL RKEDIVACIK ALVELRDGKG DVDDIDHLGN RRVRSVGELM
     ENQYRVGLLR MERAIKERMS SVEIDTVMPQ DLINAKPAAA AVREFFGSSQ LSQFMDQTNP
     LSEVTHKRRL SALGPGGLTR ERAGFEVRDV HPTHYGRMCP IETPEGPNIG LINSLATFAR
     VNKYGFIETP YRVVKDGQVT DEVHYMSATE EMRHTVAQAN ANLDEDNRFV NDLVSTRQSG
     DYTLAPNESV DLIDVSPKQL VSVAASLIPF LENDDANRAL MGSNMQRQAV PLLRAEAPLV
     GTGIEEIVAR DSGAAIMAKR AGVIDQIDAQ RIVIRATSDL ELGDAGVDIY RMRKFQRSNQ
     NTCINQRPLV KVGQTVEKGE VIADGPSTDM GELALGKNVV VAFMPWNGYN YEDSILISER
     IARDDVFTSI HIEEFEVAAR DTKLGPEEIT RDIPNVGEEA LRNLDEAGIV YIGADVEPGD
     ILVGKITPKG ESPMTPEEKL LRAIFGEKAS DVRDTSLRVK PGDYGTVVEV RVFNRHGVEK
     DERALQIERE EVERLARDRD DELGILDRNI YARLRDLLLG KTAVKGPKGV RGNTVIDEDL
     LDNQLTRGQW WMLALEEEQD AQILEALNEQ YEAQKRALDA RFEDKVEKVR RGDDLPPGVM
     KMVKVFIAVK RKLQPGDKMA GRHGNKGVIS RVVPMEDMPF LADGTPVDFC LNPLGVPSRM
     NVGQILETHM GWAARGLGLK IDDALQDYRR TGDLTPVRDA MREAYGEDVY EEGISSMDET
     QLIEAAGNVT RGVPIATPVF DGAKEDDVND ALVRAGFDQS GQSILFDGRT GEQFARPVTV
     GIKYLLKLHH LVDDKIHARS TGPYSLVTQQ PLGGKAQFGG QRFGEMEVWA LEAYGAAYTL
     QEMLTVKSDD VAGRTKVYES IVKGEDNFEA GVPESFNVLV KEVRGLGLNM ELLDAEVEE