ID   RPOB_SACD2              Reviewed;        1361 AA.
AC   Q21M93;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2006, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Sde_0924;
OS   Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales;
OC   Cellvibrionaceae; Saccharophagus.
OX   NCBI_TaxID=203122;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=2-40 / ATCC 43961 / DSM 17024;
RX   PubMed=18516288; DOI=10.1371/journal.pgen.1000087;
RA   Weiner R.M., Taylor L.E. II, Henrissat B., Hauser L., Land M.,
RA   Coutinho P.M., Rancurel C., Saunders E.H., Longmire A.G., Zhang H.,
RA   Bayer E.A., Gilbert H.J., Larimer F., Zhulin I.B., Ekborg N.A., Lamed R.,
RA   Richardson P.M., Borovok I., Hutcheson S.;
RT   "Complete genome sequence of the complex carbohydrate-degrading marine
RT   bacterium, Saccharophagus degradans strain 2-40 T.";
RL   PLoS Genet. 4:E1000087-E1000087(2008).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000282; ABD80186.1; -; Genomic_DNA.
DR   RefSeq; WP_011467407.1; NC_007912.1.
DR   AlphaFoldDB; Q21M93; -.
DR   SMR; Q21M93; -.
DR   STRING; 203122.Sde_0924; -.
DR   PRIDE; Q21M93; -.
DR   EnsemblBacteria; ABD80186; ABD80186; Sde_0924.
DR   KEGG; sde:Sde_0924; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_0_6; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000001947; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1361
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000300394"
SQ   SEQUENCE   1361 AA;  151592 MW;  E6EBB678AAEFF8D3 CRC64;
     MAYSYTEKKR IRKDFGKLPD VMEVPYLLAI QLDSYRKFTQ ADTPFDQRKD MGLHAAFKSI
     FPIVSYSGNA ALEYVSYALG KPVFDVKECV LRGATYAVPL RVKVRLIIYD KDSSSKAIKD
     IKEQEVYMGE IPLMTDNGTF VVNGTERVIV SQLHRSPGVF FEHDKGKTHS SGKLLYSARV
     IPYRGSWLDF EFDPKDLVFV RIDRRRKLPA SILLRALGYT TEQMLDMFYE TSKFGLTETG
     ECVLELVPSR LRGDVTTFEI KDKDGNLIVE DGRRITARHI RQLEKAGVTE LSVPHEYMVG
     RALAKDIINA ESGEVLFECN TEITDEVLAE LVKSGVKEFE TLYTNELDCG PFISDTLRSD
     PARTQLEALV EIYRMMRPGE PPTKESAETL FQNLFFSSER YDLSAVGRMK FNRRLGRDSE
     VGSGTLSNED IVDVLKTLID IRNGKGVVDD IDHLGNRRVR SVGEMAENQF RVGLVRVERA
     VKERLSMAES EGLMPQDLIN AKPVAAAVKE FFGSSQLSQF MDQNNPLSEV THKRRVSALG
     PGGLTRERAG FEVRDVHPTH YGRVCPIETP EGPNIGLINS LATYARTNSY GFLESPHRKV
     VDGKVTDEIE YLSAINEAKF VIAQASAAQN ENGELTEELV SVRFQNEFTL KGPSEVQYMD
     VSPRQVVSVA ASLIPFLEHD DANRALMGSN MQRQAVPTLK AQKPLVGTGM ERNVAADSGV
     CVVAKRGGVI ERVDAARIVV RVADDEVEAG EAGVDIYNLT KYTRSNQNTC INQRSIVRTG
     EKVSRGDILA DGPSVDLGEL ALGQNMRIAF MPWNGYNFED SILVSERVVQ EDRFTTIHIQ
     ELTCIARDTK LGSEEITADI PNVGEGALSK LDESGIVYVG AEVASGDILV GKVTPKGETQ
     LTPEEKLLRA IFGEKASDVK DTSLRVPSSV KGTVIDVQVF TRDGLEKDQR SRDIEKAQLD
     QIRKDLNEEY RIVQGATFER LRAALVGNTA MTGKGVVKGQ PVTDEMLDEL SHDDWFKIRM
     NDDVLNEQID AAEVALAERR KELDDRFEDK KGKLETGDDL APGVLKIVKV YLAIKRRIQP
     GDKMAGRHGN KGVISVIMPV EDMPYDENGN PIDIVLNPLG VPSRMNVGQV LEMHLGLAAK
     SLGEKIDEML KQQKAVAEIR QFLDQIYNQI GESYKAEELD TFSDDEIIEL ANNLRDGVPM
     ATRAFDGAKE KEIKQLLTLG GMPDSGQMTL FDGRTGDPFQ RPTTVGYMYM LKLNHLVDDK
     MHARSTGSYS LVTQQPLGGK AQFGGQRFGE MEVWALEAYG AAYTLQEMLT VKSDDVAGRT
     KMYKNIVDGD HRMEPGMPES FNVLVKEIRS LGINIELEQD N