RPOB_SACEN
ID RPOB_SACEN Reviewed; 1164 AA.
AC A4FPP3;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 17-APR-2007, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=SACE_6854;
OS Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 /
OS NBRC 13426 / NCIMB 8594 / NRRL 2338).
OC Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
OC Saccharopolyspora.
OX NCBI_TaxID=405948;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL
RC 2338;
RX PubMed=17369815; DOI=10.1038/nbt1297;
RA Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S.,
RA Haydock S.F., Leadlay P.F.;
RT "Complete genome sequence of the erythromycin-producing bacterium
RT Saccharopolyspora erythraea NRRL23338.";
RL Nat. Biotechnol. 25:447-453(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; AM420293; CAM06018.1; -; Genomic_DNA.
DR RefSeq; WP_011875176.1; NZ_PDBV01000001.1.
DR AlphaFoldDB; A4FPP3; -.
DR SMR; A4FPP3; -.
DR STRING; 405948.SACE_6854; -.
DR PRIDE; A4FPP3; -.
DR EnsemblBacteria; CAM06018; CAM06018; SACE_6854.
DR KEGG; sen:SACE_6854; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_11; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000006728; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1164
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300395"
SQ SEQUENCE 1164 AA; 128683 MW; 02A09D0348FC2564 CRC64;
MAVSRATKVS AASNMMSGIP GAPKRVSFAN IREPLEVPDL LDLQVQSFEW LVGDETWFQR
RVDAGDENPV GGLEEVLSEI SPIEDFSGSM SLSFSDPRFD EVKASVEECK DKDMTYAAPL
FVTAEFTNHT TGEIKSQTVF MGDFPVMTDK GTFIINGTER VVVSQLVRSP GVYFDQSVDK
TTDKDVYSVK IIPSRGAWLE FDVDKRDTVG VRIDRKRRQP VTVLLKALGW TAEAIRERFG
FSETLMATLE KDHTAGQDEA LLDIYRKLRP GEPPTKESAQ TLLENLFFKE KRYDLARVGR
YKVNKKLGLD LPFESGVLTE EDIVTTIEYL VRLHAGEAEM PDRGQGGDAT IPVEVDDIDH
FGNRRLRTVG ELIQNQVRVG LSRMERVVRE RMTTQDVEAI TPQTLINIRP VVAAIKEFFG
TSQLSQFMDQ TNPIAGLTHK RRLSALGPGG LSRERAGMEV RDVHPSHYGR MCPIETPEGP
NIGLIGSLAT FARVNPFGFI ETPYRKVVDG RVTDQIDYLT ADEEDRFVKA QANTPIDDEG
NFLADRVLGR RKGGEVELLA PTEIDYMDVS PRQMVSAATA MIPFLEHDDA NRALMGANMQ
RQAVPLLRSE APLVGTGMEL RAAVDAGDVV TAEKAGVIEE LCADYITVMA DDGTRRTYRL
QKFSRSNHGT CTNQKPIVNE GDRVEAGQVI ADGPCTQNGE MALGKNLRVA IMPWEGHNYE
DAIILSQRLV QDDVLTSIHI EEHEVDARDT KLGAEEITRD IPNVSEDVLA DLDERGIIRI
GAEVQGGDIL VGKVTPKGET ELTPEERLLR AIFGEKAREV RDTSLKVPHG ETGKVIGVRV
FNREDDDELP PGVNELVRVY VAQKRKIQDG DKLAGRHGNK GVIGKILPAE DMPFTEDGTP
VDIILNTHGV PRRMNIGQIL ETHLGWIASQ GWSIDGDPEW AKRLPEELYD VDPGTNTASP
VFDGAREEEI TGLLASTKPN RDGERMVKAN GKAQLFDGRS GEPYPYPVAV GYMYILKLSH
LVDDKIHARS TGPYSMITQQ PLGGKAQFGG QRFGEMECWA MQAYGAAYTL QELLTIKSDD
VVGRVKVYEA IVKGENIPEP GIPESFKVLL KELQSLCLNV EVLSSDGAAI EMRDGDDEDL
ERAAANLGIN LSRNESPSVD DVVQ
