ID   RPOB_SACOF              Reviewed;        1075 AA.
AC   Q6ENX3;
DT   21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS   Saccharum officinarum (Sugarcane).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Saccharinae; Saccharum;
OC   Saccharum officinarum complex.
OX   NCBI_TaxID=4547;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15449542; DOI=10.1093/dnares/11.2.93;
RA   Asano T., Tsudzuki T., Takahashi S., Shimada H., Kadowaki K.;
RT   "Complete nucleotide sequence of the sugarcane (Saccharum officinarum)
RT   chloroplast genome: a comparative analysis of four monocot chloroplast
RT   genomes.";
RL   DNA Res. 11:93-99(2004).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AP006714; BAD27283.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q6ENX3; -.
DR   SMR; Q6ENX3; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 3.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1075
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000048045"
SQ   SEQUENCE   1075 AA;  121537 MW;  C97CA048CE2C2F75 CRC64;
     MLRNGNEGMS TIPGFSQIQF EGFCRFINQG LAEELEKFPT IKDPDHEIAF QLFAKGYQLL
     EPSIKERNAV YESLTYSSEL YVSARLIFGF DVQKQTISIG NIPIMNSLGT FIINGIYRIV
     INQILLSPGI YYRSELDHKG ISICTGTIIS DWGGRSELAI DKKERIWARV SRKQKISILV
     LSSAMGSNLR EILDNVSYPE IFLSFPNAKE KKRIESKEKA ILEFYQQFAC VGGDLVFSES
     LCEELQKKFF QQKCELGRVG RRNMNRRLNL DIPQNNTFLL PRDVLAATDH LIGMKFGTGI
     LDDDDMNHLK NKRIRSVADL LQDQFGLALG RLQHAVQKTI RRVFIRQSKP TPQTLVTPTS
     TSILLITTYE TFFGTYPLAQ VFDQTNPLTQ TVHGRKVSCL GPGGLTGRTA SFRSRDIHPS
     HYGRICPIDT SEGINVGLTG SLAIHARIDH WWGSIESPFY EISEKAKEKK ERQVVYLSPN
     RDEYYMIAAG NSLSLNQGIQ EEQVVPARYR QEFLTIAWEQ IHVRSIFPFQ YFSIGGSLIP
     FIEHNDANRA LMSSNMQRQA VPLSRSEKCI VGTGLERQTA LDSRVSVIAE REGKIISSDS
     HKILLSSSGK TISIPLVAHR RSNKNTCMHQ KPRVPRGKSI KKGQILAEGA ATVGGELALG
     KNVLVAYMPW EGYNFEDAVL ISERLVYEDI YTSFHIRKYE IQTDTTSQGS AEKITKQIPH
     LEEHLLRNLD RNGVVRLGSW VETGDILVGK LTPQIASESS YIAEAGLLRA IFGLEVSTSK
     ETSLKLPIGG RGRVIDVKWI QRDPFDIMVR VYILQKREIK VGDKVAGRHG NKGIISKILP
     RQDMPYLQDG TPVDMVFNPL GVPSRMNVGQ IFESSLGLAG DLLKKHYRIA PFDERYEQEA
     SRKLVFSELY EASKQTKNPW VFEPEYPGKS RIFDGRTGDP FEQPVLIGKS YILKLIHQVD
     EKIHGRSTGP YSLVTQQPVR GRAKQGGQRI GEMEVWALEG FGVAHILQEI LTYKSDHLIA
     RQEILNATIW GKRVPNHEDP PESFRVLVRE LRSLALELNH FLVSEKNFRV NREDV