ID   RPOB_SAPOF              Reviewed;         417 AA.
AC   P08036;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1988, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta;
DE            EC=2.7.7.6;
DE   AltName: Full=PEP;
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta;
DE            Short=RNA polymerase subunit beta;
DE   Flags: Fragment;
GN   Name=rpoB;
OS   Saponaria officinalis (Common soapwort) (Lychnis saponaria).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Caryophyllaceae; Caryophylleae; Saponaria.
OX   NCBI_TaxID=3572;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Leaf;
RX   PubMed=3368320; DOI=10.1093/nar/16.7.3103;
RA   Benatti L., Lorenzetti R., Dani M., Martini D., Minganti C., Sasano M.,
RA   Sidoli A., Soria M.;
RT   "A DNA sequence from Saponaria officinalis is similar to various RNA
RT   polymerase genes.";
RL   Nucleic Acids Res. 16:3103-3103(1988).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000305}.
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DR   EMBL; X07026; CAA30075.1; -; Genomic_DNA.
DR   PIR; S00933; S00933.
DR   AlphaFoldDB; P08036; -.
DR   SMR; P08036; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 2.40.270.10; -; 3.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           <1..>417
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000048046"
FT   NON_TER         1
FT   NON_TER         417
SQ   SEQUENCE   417 AA;  46638 MW;  2AF8535B23B35A88 CRC64;
     EKCIVGTGLE RQAALDSGVL AIVEHEGKII YTDTDKIILS GNGDTHSIPL VLYQRFNKNT
     CMHQNPRIPG GKCIKKGQIL ADGAATVGGE LGLGKNVLVA YMPWEGYNFE DAVLISERLV
     YEDIYTSFHI RKYEIQTYVT SQGPERVTSE IPHLEAHLLR NLDKNGIVGL GSWVETGDIL
     VGKLTPQMAK ESSYAPEDRL LRAILGIQVS TSKETCLKLP IGGRGRVIDV RWIQKKGGSN
     YNPETIHIYI LQKREIKVGD KVAGRHGNKG IISKILPRQD MPYLQDGRPV DMVFNPLGVP
     SRMNVGQIFE CSLGLAGGLL DRHYRITPFD ERYEHEASRK LVFSELYQAS KQTAKPWIFE
     PEYPGKSRIF DGRTGDPFEQ PVIIGNPYIL KLIHQVDDKI HGRSSGHYAL VTQQPLR