ID   RPOB_SHEON              Reviewed;        1345 AA.
AC   Q8EK74;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=SO_0224;
OS   Shewanella oneidensis (strain MR-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=211586;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MR-1;
RX   PubMed=12368813; DOI=10.1038/nbt749;
RA   Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C.,
RA   Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A.,
RA   Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C.,
RA   DeBoy R.T., Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R.,
RA   Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J.,
RA   Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J.,
RA   Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V.,
RA   Smith H.O., Venter J.C., Nealson K.H., Fraser C.M.;
RT   "Genome sequence of the dissimilatory metal ion-reducing bacterium
RT   Shewanella oneidensis.";
RL   Nat. Biotechnol. 20:1118-1123(2002).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AE014299; AAN53309.1; -; Genomic_DNA.
DR   RefSeq; NP_715864.1; NC_004347.2.
DR   RefSeq; WP_011070610.1; NZ_CP053946.1.
DR   AlphaFoldDB; Q8EK74; -.
DR   SMR; Q8EK74; -.
DR   STRING; 211586.SO_0224; -.
DR   PaxDb; Q8EK74; -.
DR   PRIDE; Q8EK74; -.
DR   KEGG; son:SO_0224; -.
DR   PATRIC; fig|211586.12.peg.212; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_1_6; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   PhylomeDB; Q8EK74; -.
DR   BioCyc; SONE211586:G1GMP-213-MON; -.
DR   Proteomes; UP000008186; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1345
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000047954"
SQ   SEQUENCE   1345 AA;  150295 MW;  7110B0E3D32A8ACA CRC64;
     MVYSYSEKKR IRKDFGKRPQ VLDIPYLLSI QLDSFKKFTD QDPTGERGLE AAFRSVFPIK
     SFSGNSELQY VSYKLGEPVF DVKECQIRGV TYSAPLRVKL RMVLYDREAA AGTVKDIKEQ
     EVYMGDIPLM TDNGTFVING TERVIVSQLH RSPGVFFDHD RGKTHSSGKV LYNARIIPYR
     GSWLDFEFDP KDALFVRIDR RRKLPATIIL RALEYSTQEI LDLFFERVEF KIKKDTLVMT
     LVPERLRGET ASYDIKDAEG TVLVEAGRRV TARHIRQLEK TNTTELEVPV EYIVGKYAAQ
     DYIDPDTGEV LVSANSEISL EDLAKLSLAG IKELSTLYIN ELDHGAYISD TLRIDPTTNR
     LEALVEIYRM MRPGEPPTKD AAEALFQNLF FSEERYDLSK VGRMKFNRRL SIPDDEGSGV
     LSKEDIVAVM KNIIHIRNGF DEVDDIDHLG NRRIRSVGEM AENQFRVGLV RVERAVRERL
     SLGDLNELMP QDLINAKPIS AAVKEFFGSS QLSQFMDQNN PLSEVTHKRR ISALGPGGLT
     RERAGFEVRD VHPTHYGRLC PIETPEGPNI GLINSLASFA RTNSYGFLET PYRKVVDGVI
     TDEVEYLSAI EEGRYVIAQA NIEVDSEGRM VEEQIACRHK GESTFMRASD IQYMDVSPQQ
     IISVAASLIP FLEHDDANRA LMGANMQRQA VPTLRSEKPL VGTGIERTLA VDSGVVVAAK
     RGGVIDYVDA SRIVVKVNED ELRPGEAGID IYNLTKYTRS NQNTCINQRP CCSVGEPVVR
     GDVLADGPST DLGDLALGQN MRIAFMPWNG YNFEDSILIS ERVAQEDRFT TIHIQELSCI
     ARDTKLGSEE ITADIPNVGE SALSKLDESG IVYIGAEVKG GDILVGKVTP KGETQLTPEE
     KLLRAIFGEK ASDVKDSSLR VPNSVKGTII DVQVFTRDGV EKDKRAIEIE EMHIAQARKD
     LGEEFKILEE GVLSRARNLL LSAGFSEAQI AALPRKDVLV QVIDDEAKQT ELEQLAEQHE
     ELKADFDKKF EIKRRKITQG DDLAPGVLKI VKVYLAVKRT IQPGDKMAGR HGNKGVISKI
     NPIEDMPYDE QGNPVDIVLN PLGVPSRMNI GQVLEVHLGA AAKGIGHKIA AMLEDQREKG
     LAEVRNYIKQ VYELGDEVQQ RVDIDSFTDD ELLRLANNLK GGIPVATPAF DGAKEKEIKQ
     MLELAGLPTS GQLKLFDGRT GNEFERPVTV GYMYMLKLNH LVDDKMHARS TGSYSLVTQQ
     PLGGKAQFGG QRFGEMEVWA LEAYGAAYTL QEMLTVKSDD VNGRTQMYKN IVDGNHQMQP
     GMPESFNVLL KEIRSLGINI ELDQE