RPOB_SHEPA
ID RPOB_SHEPA Reviewed; 1343 AA.
AC A8GYW9;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Spea_0177;
OS Shewanella pealeana (strain ATCC 700345 / ANG-SQ1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC Shewanellaceae; Shewanella.
OX NCBI_TaxID=398579;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700345 / ANG-SQ1;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C.,
RA Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.,
RA Zhao J.-S.Z., Manno D., Hawari J., Richardson P.;
RT "Complete sequence of Shewanella pealeana ATCC 700345.";
RL Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000851; ABV85506.1; -; Genomic_DNA.
DR RefSeq; WP_012153452.1; NC_009901.1.
DR AlphaFoldDB; A8GYW9; -.
DR SMR; A8GYW9; -.
DR STRING; 398579.Spea_0177; -.
DR EnsemblBacteria; ABV85506; ABV85506; Spea_0177.
DR KEGG; spl:Spea_0177; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_3_6; -.
DR OMA; FMTWEGY; -.
DR Proteomes; UP000002608; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 2.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1343
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000086381"
SQ SEQUENCE 1343 AA; 149715 MW; D35D6C67FD556DC1 CRC64;
MVYSYSEKKR IRKDFGKRPR VLDIPYLLSI QLDSFKKFTD QDPTGERGFE AAFRSVFPIK
SFSGNSELQY VSYKLGEPVF DVKECQIRGV TYSAPLRVKL RMVLYDREAA PGTVKDIKEQ
EVYMGDIPLM TDNGTFVING TERVIVSQLH RSPGVFFDHD RGKTHSSGKV LYNARIIPYR
GSWLDFEFDP KDALFVRIDR RRKLAASIIL RALDYSTQDI LDLFFDRVNF KIKKDSLVMD
LVADRLRGET ASYDIKDAEG TVLVEKGRRI TARHIRQLEK TNTTELEVPV EYIAGKISGQ
DYIDPDTGEV LVSANADISL EDLAKLSMAG IKEISTLYIN ELDNGAYISD TLRIDSTTNR
LEALVEIYRM MRPGEPPTKD AAEALFNNLF FSEERYDLSK VGRMKFNRRL SIDDDEGTGI
LSKEDIVAVM KNIIAIRNGF DEVDDIDHLG NRRIRSVGEM AENQFRVGLV RVERAVRERL
SLGDLNELMP QDLINAKPIS AAVKEFFGSS QLSQFMDQNN PLSEVTHKRR ISALGPGGLT
RERAGFEVRD VHPTHYGRLC PIETPEGPNI GLINSLSTFA RTNNYGFLET PYRKVIDGVV
TDEVDYLSAI EEGRYVIAQA IVQLDENGRM MDELIACRHK GDSTFMGAAD IQYMDVSPQQ
IISVAASLIP FLEHDDANRA LMGANMQRQA VPTLKADKPL VGTGIERTLA VDSGVVVAAK
RGGYVDYVDA SRIVVKVNEA ELTPGEAGID IYNLTKYTRS NQNTCINQRP CCSVGDPIVR
GDVLADGPST DLGDLALGQN MRIAFMPWNG YNFEDSILIS ERVAQEDRFT TIHIQELSCI
ARDTKLGSEE ITADIPNVGE SALSKLDESG IVYIGAEVKG GDILVGKVTP KGETQLTPEE
KLLRAIFGEK ASDVKDSSLR VPNSVKGTII DVQVFTRDGV EKDKRAVEIE EMHIAQAKKD
LTEEFQILED GVIGRARNLL IGAGFDEAQL AAIPRSQLLV QTIDDEVKQT ELEQLAEQAE
ELKADFDKKF EIKRRKITQG DDLAPGVLKI VKVYLAVKRT IQPGDKMAGR HGNKGVISKI
CPVEDMPYDE KGNPVDIVLN PLGVPSRMNI GQVLEVHMGA AAKGIGNRIT EMLEEQRELA
ELRGYIKQVY ELGDEVIQKV DIDSFTDDEI LRLAKNLKGG VPIATPAFDG AKEKEIKQML
ELAGLPQSGQ LTLIDGRTGN EFERKVTVGY MYMLKLNHLV DDKMHARSTG SYSLVTQQPL
GGKAQFGGQR FGEMEVWALE AYGAAYTLQE MLTVKSDDVN GRTQMYKNIV DGNHQMQPGM
PESFNVLLKE IRSLGINIEL DTK
