ID   RPOB_SINFN              Reviewed;        1380 AA.
AC   C3MAX2;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=NGR_c11830;
OS   Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=19376903; DOI=10.1128/aem.00515-09;
RA   Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA   Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA   Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA   Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT   "Rhizobium sp. strain NGR234 possesses a remarkable number of secretion
RT   systems.";
RL   Appl. Environ. Microbiol. 75:4035-4045(2009).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP001389; ACP24965.1; -; Genomic_DNA.
DR   RefSeq; WP_012707748.1; NC_012587.1.
DR   RefSeq; YP_002825718.1; NC_012587.1.
DR   AlphaFoldDB; C3MAX2; -.
DR   SMR; C3MAX2; -.
DR   STRING; 394.NGR_c11830; -.
DR   PRIDE; C3MAX2; -.
DR   EnsemblBacteria; ACP24965; ACP24965; NGR_c11830.
DR   KEGG; rhi:NGR_c11830; -.
DR   PATRIC; fig|394.7.peg.3999; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_0_5; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000001054; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 2.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 2.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1380
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_1000165816"
SQ   SEQUENCE   1380 AA;  153442 MW;  028342BFAEA0A6D5 CRC64;
     MAQTLSFNGR RRVRKFFGKI PEVAEMPNLI EVQKASYDQF LMVEEPAGGR PDEGLQAVFK
     SVFPIKDFSG ASMLEFVSYE FEPPKFDVEE CRQRDLTYAA PLKVTLRLIV FDIDEDTGAK
     SIKDIKEQNV YMGDMPLMTD NGTFIVNGTE RVIVSQMHRS PGVFFDHDKG KSHSSGKLLF
     AARVIPYRGS WLDIEFDAKD IVHARIDRRR KIPVTSLLMA LGMDGEEILS TFYTQSLYQR
     DGDGWRVPFQ PDALKGQKAI AEMIDADTGE VVVEAGKKLT PRLLRQLQEK GLKALKSTDD
     ELYGNYLAED VVNYETGEIY LEAGDEIDEK TLPVILSAGF DEIPVLDIDH INVGAYIRNT
     LAADKNENRQ DALFDIYRVM RPGEPPTMDS AEAMFNALFF DAERYDLSAV GRVKMNMRLD
     LDVPDTVRTL RKEDILAVVK MLVELRDGKG EIDDIDNLGN RRVRSVGELM ENQYRLGLLR
     MERAIKERMS SIEIDTVMPQ DLINAKPAAA AVREFFGSSQ LSRFMDQVNP LSEITHKRRL
     SALGPGGLTR ERAGFEVRDV HPTHYGRICP IETPEGPNIG LINSLATFAR VNKYGFIESP
     YRKIVDGNVT SDVVYLSAME DAKYHVAQAN SVLNDDGSFA EEFVVCRHAG EVMLAPRDNI
     NLMDVSPKQL VSVAAALIPF LENDDANRAL MGSNMQRQAV PLLRAEAPFV GTGMESVVAR
     DSGAAIAARR GGVVDQVDAT RIVIRATEDL DPSKSGVDIY RLQKFQRSNQ NTCVNQRPLV
     TVGDLVNKGD IIADGPSTDL GDLALGRNAL VAFMPWNGYN YEDSILLSER IVRDDVFTSI
     HIEEFEVMAR DTKLGPEEIT RDIPNVSEEA LKNLDEAGIV YIGAEVQPGD ILVGKITPKG
     ESPMTPEEKL LRAIFGEKAS DVRDTSMRMP PGTFGTIVEV RVFNRHGVEK DERAMAIERE
     EIERLAKDRD DEQAILDRNV YARLIDMLRG HVAVAGPKGF KKGTELSNAV ISEYPRSQWW
     MFAVEDEKAQ GEIEALRAQY DESKSRLEQR FMDKVEKVQR GDEMPPGVMK MVKVFVAVKR
     KIQPGDKMAG RHGNKGVVSR IVPIEDMPFL EDGTHVDVVL NPLGVPSRMN VGQILETHLG
     WACAGMGKKI GAMLDAYHES GDIKPLRTTI DDVIGSGPKG EPIKQYDDES IVRLAEQTRR
     GVSIATPVFD GAVEADVNEM LEKAGLKVTG QSTLYDGRTG DAFDRQVTVG YIYMLKLNHL
     VDDKIHARSI GPYSLVTQQP LGGKAQFGGQ RFGEMEVWAL EAYGAAYTLQ EMLTVKSDDV
     AGRTKVYEAI VRGDDTFEAG IPESFNVLVK EMRSLGLSVE LENSKVDDLG TTAQLPDAAE