RPOB_SYNFM
ID RPOB_SYNFM Reviewed; 1370 AA.
AC A0LII4;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 12-DEC-2006, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Sfum_1549;
OS Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB).
OC Bacteria; Proteobacteria; Deltaproteobacteria; Syntrophobacterales;
OC Syntrophobacteraceae; Syntrophobacter.
OX NCBI_TaxID=335543;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 10017 / MPOB;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E.G.,
RA Martinez M., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA Kim E., Boone D.R., Brockman F., Culley D., Ferry J., Gunsalus R.,
RA McInerney M.J., Morrison M., Plugge C., Rohlin L., Scholten J., Sieber J.,
RA Stams A.J.M., Worm P., Henstra A.M., Richardson P.;
RT "Complete sequence of Syntrophobacter fumaroxidans MPOB.";
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000478; ABK17236.1; -; Genomic_DNA.
DR RefSeq; WP_011698407.1; NC_008554.1.
DR AlphaFoldDB; A0LII4; -.
DR SMR; A0LII4; -.
DR STRING; 335543.Sfum_1549; -.
DR PRIDE; A0LII4; -.
DR EnsemblBacteria; ABK17236; ABK17236; Sfum_1549.
DR KEGG; sfu:Sfum_1549; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_7; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000001784; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1370
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300420"
SQ SEQUENCE 1370 AA; 153980 MW; AF94B458D1A33454 CRC64;
MPTALTHEYR VRKNFGKIHK IIDIPNLIQM QKESYELFLQ RDVPSEARSE RGLQEVFKSV
FPIEDFSGTA SLEFVQYSFG EVKYEVEECL ARGMTYEAPV KIVVRLVVYD VDKEAGTRSI
RDIKEQEIYF GTLPLMTDNG TFIINGTERV IVSQLHRSPG IFFDHDRGKT HSSGKILYSA
RIIPLRGSWL DLEFDPKDIL YIRIDRRRKF PVTVLLKALG YATEELLNYF YPSEKIFLKS
EAQSEKELNP DILMGSRAPE DILHPQTGEV LIKKNRKLGK QALRRLQEVG INRLPMKSTE
LIGQVLAQDV IDYGTGEIVA ECNDSIDADM LKEFVERGVG EIELLHLEGQ DVSPSFRNTL
LMDKVNTQED ALIEIYRRLR PSNPPTLEVA TEFFNNLFFN PDHYDLSEVG RLKLNLQLGL
EVPLDYRTLR KDDILMAVRQ LIRLKDSQGP VDDIDNLGNR RVRAVGELLE NQYRIGLVRM
ERAIKERMTL QEVEALMPHD LINAKPVSAV VKEFFGTSQL SQFMDQTNPL SEITHKRRLS
ALGPGGLTRE RAGFEVRDVH PTHYGRICPI ETPEGPNIGL IVSLSTYARV NPYGFIETPY
RKVDGSSARK DVSYLTAMDE KEYPIAQANA PLDEKGRFLL DLVSARVAGE PVMVPPNEIR
YMDVSPNQLV SVSASLIPFL EHDDANRALM GSNMQRQAVP LIQTRAPLVG TGIERIVAKD
SGVAIVARRT GVVEYVDATR IVIRAIEDNG EMGSGVDIYK LIKFQRSNQN TCINQKPLVQ
HGDLVTKGQI IADGPSTDHG ELALGRNVMV AFMSWGGYNF EDSILVSERI GKEDVFTSIH
IEEFEVVARD TKLGKEDITR DIPNVGEEAL KNLDESGIIR VGAYIKPNDI LVGKVTPKGE
SQLTPEEKLL RAIFGEKASD VKDTSLRVPP GVEGIIIDAK VFSRKGVEKD ERTKTIEDQE
ISRLMKDQRD ELEIIFKSTV KRLAKLLENQ VSDSAIKDGK KVYIKKGEVF TEEILLNLPS
GHWDQLTVAK DPTVSMEIEN ILANYREQVQ LVKSLFEEKI GKLKRGDELP PGVIKMVKVY
VAVKRKLQVG DKMAGRHGNK GVVSRILPAE DMPYFPDGTP VDIVLNPLGV PSRMNVGQVL
ETHIGWAAKG IGMQLAQMLE ECRERETMNE KLQRIYNKLE FGTYFKDASE RELRGLIPDF
KEGIHVASPV FDGAEEAEIR AFLSEAGVSE TGQSVLYDGR TGMPFDQPVT VGVMYMLKLH
HLVDDKIHAR SIGPYSLVTQ QPLGGKAQFG GQRLGEMEVW TMEAYGAAYA LQEFLTVKSD
DVAGRTRMYE KIVKGDNTLE AGLPESFNVL VKELQALALD VRLLEEEEGN
