RPOB_THAPS
ID RPOB_THAPS Reviewed; 1386 AA.
AC A0T0R1;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS Thalassiosira pseudonana (Marine diatom) (Cyclotella nana).
OG Plastid; Chloroplast.
OC Eukaryota; Sar; Stramenopiles; Ochrophyta; Bacillariophyta;
OC Coscinodiscophyceae; Thalassiosirophycidae; Thalassiosirales;
OC Thalassiosiraceae; Thalassiosira.
OX NCBI_TaxID=35128;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCMP1335 / NEPCC58 / CCAP 1085/12;
RX PubMed=17252281; DOI=10.1007/s00438-006-0199-4;
RA Oudot-Le Secq M.-P., Grimwood J., Shapiro H., Armbrust E.V., Bowler C.,
RA Green B.R.;
RT "Chloroplast genomes of the diatoms Phaeodactylum tricornutum and
RT Thalassiosira pseudonana: comparison with other plastid genomes of the red
RT lineage.";
RL Mol. Genet. Genomics 277:427-439(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; EF067921; ABK20746.1; -; Genomic_DNA.
DR RefSeq; YP_874523.1; NC_008589.1.
DR AlphaFoldDB; A0T0R1; -.
DR SMR; A0T0R1; -.
DR STRING; 35128.Thapsdraft1267; -.
DR GeneID; 4524729; -.
DR eggNOG; KOG0214; Eukaryota.
DR InParanoid; A0T0R1; -.
DR Proteomes; UP000001449; Chloroplast.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 2.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW Reference proteome; Transcription; Transferase.
FT CHAIN 1..1386
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000276600"
SQ SEQUENCE 1386 AA; 159979 MW; 10C2AACFFE18FCF7 CRC64;
MNYTTALPDF IEMQRVSFCW FIAQGLNEEL NTFSRIYDFS QNTEYVLFGQ EYSLVKPIYN
IVRAKKYTAN YSAQLVIPLE VRNKKTNIIK YHSKFPIINL PLMTSSATFV INGCERVIVS
QIIRSPGVYF EKNKHQKKNK KIRKLISSEI GKLKSFTPPS EILPTESRLY FLKPTIKKKL
ISDKKKKIRF IKKEEYWNWK GESLNIYSFK QIKDYEIDFS TSFVEHFKVY NDLFKIRHLS
TKIKRIKIFL KWLACNNKFS LPNKKHLIFL LINSFNLLSR TIVKYKILKT RNNENKNDFI
LNDYNQIKKV YDQVLQESET LLSLNINTQI LSFILRDLEK FNQLNNFNFL KLNITPKIKV
IIANDKIKSS LYFTNSFKEL IKFKYNFTKK EKDKKRNQSQ ATIFKNKTKY LKTKSKIIQY
KDDHLIRDIY KKKYEEKELY TATLIPEYGS WIRFGFQKNT KINISKYPIK NQEDEIIIQL
DKVTQKPIIH LLKEMGVTDL EICQNLQNSE FFYFNKPILT NSIHQPNKLL RFNLNKNYYK
NISEFSRIFD PSYYRLGKIG RSKLNNRLDI NLSKRITTIT YEDIFAIIDK LITLSTAKQI
SDDIDHLKNR RVRSVGELLQ NLFRIGFQRL LRKLRSQTNK TYSSQLSSFN IVGATIKEFF
GASQLSQYMD QTNPLSSLTH RRRISGLGPG GFDRDRISFA VRDIHPSHYG RICPIETPEG
QNVGLIASLT TCARVNESGF LETPFWRVIN GKVIKNRNPI YLTADIEDFY KIAPADISTN
QENYLTKNLI PVRYKQDFLT VTPSEVDFIA VSPIQVVSVA ASLIPFFEHD DANRALMGSN
MQRQSVPLLL PQKPIVGTGL ENQIALDSGM VINAQRAGIV ESVTANKIII LEDSGRHYKY
DLQKYQRSNQ ETCINHRPIV WEGEKVKSGQ MLTDGPGIIS SELSLGQNVL VAYMPWQGYN
FEDAILINER LVYEDIFTSI HIERYEIEID RTAEMSEQTT NNIPNLSISD VQNLNEDGIV
TIGTFVKPGD ILVGKIIPKD DSEQLPESKL LRAIFGAKAK GVRDTSFRMP EGEYGRVIET
LTFNRRTKLA YKFEKIQIFI AQIRKIQVGD KIAGRHGNKG IISRILPRQD MPFLPDGTPV
DIILNPLGVP SRMNVGQLYE CLLGLAGHKL NRRFKILPFD EMYGPEVSRI LINKKLRQAS
IENDEAWLFN PYSPGKMVLL DGRTGKEFDN PITVGNAYML KLIHLVDDKM HSRATGPYSL
VTQQPLGGKA QHGGQRFGEM EVWALEGFGA SFTLKELLTI KSDDMQGRNE TLNSIIKGQP
IPTSGVPESF KVLVQELRSI GLDLSTYRID EFSSNQSYEI ELNLIEKYNP LLKTFSHTSN
INNISF
