RPOB_THEAQ
ID RPOB_THEAQ Reviewed; 1119 AA.
AC Q9KWU7;
DT 27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS Thermus aquaticus.
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=271;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND X-RAY CRYSTALLOGRAPHY (3.3
RP ANGSTROMS).
RX PubMed=10499798; DOI=10.1016/s0092-8674(00)81515-9;
RA Zhang G., Campbell E.A., Minakhin L., Richter C., Severinov K., Darst S.A.;
RT "Crystal structure of Thermus aquaticus core RNA polymerase at 3.3 A
RT resolution.";
RL Cell 98:811-824(1999).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; Y19223; CAB65465.2; -; Genomic_DNA.
DR PDB; 1HQM; X-ray; 3.30 A; C=1-1119.
DR PDB; 1I6V; X-ray; 3.30 A; C=1-1119.
DR PDB; 1L9U; X-ray; 4.00 A; C/L=1-1119.
DR PDB; 1L9Z; X-ray; 6.50 A; C=1-1119.
DR PDB; 1YNJ; X-ray; 3.20 A; C=1-1119.
DR PDB; 1YNN; X-ray; 3.30 A; C=1-1119.
DR PDB; 2GHO; X-ray; 5.00 A; C=1-1119.
DR PDB; 3MLQ; X-ray; 2.91 A; A/B/C/D=17-139, A/B/C/D=334-395.
DR PDB; 4XAX; X-ray; 2.40 A; A=17-139, A=334-392.
DR PDB; 4XLN; X-ray; 4.00 A; C/I=1-1119.
DR PDB; 4XLP; X-ray; 4.00 A; C/I=1-1119.
DR PDB; 4XLQ; X-ray; 4.60 A; C/I=1-1119.
DR PDB; 4XLR; X-ray; 4.30 A; C/I=1-1119.
DR PDB; 4XLS; X-ray; 4.01 A; C/I=1-1119.
DR PDB; 5TJG; X-ray; 2.60 A; C=1-1119.
DR PDBsum; 1HQM; -.
DR PDBsum; 1I6V; -.
DR PDBsum; 1L9U; -.
DR PDBsum; 1L9Z; -.
DR PDBsum; 1YNJ; -.
DR PDBsum; 1YNN; -.
DR PDBsum; 2GHO; -.
DR PDBsum; 3MLQ; -.
DR PDBsum; 4XAX; -.
DR PDBsum; 4XLN; -.
DR PDBsum; 4XLP; -.
DR PDBsum; 4XLQ; -.
DR PDBsum; 4XLR; -.
DR PDBsum; 4XLS; -.
DR PDBsum; 5TJG; -.
DR AlphaFoldDB; Q9KWU7; -.
DR SMR; Q9KWU7; -.
DR PRIDE; Q9KWU7; -.
DR EvolutionaryTrace; Q9KWU7; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW Transcription; Transferase.
FT CHAIN 1..1119
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000047983"
FT STRAND 3..5
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 18..30
FT /evidence="ECO:0007829|PDB:4XAX"
FT TURN 36..38
FT /evidence="ECO:0007829|PDB:4XAX"
FT STRAND 41..43
FT /evidence="ECO:0007829|PDB:1YNN"
FT HELIX 44..51
FT /evidence="ECO:0007829|PDB:4XAX"
FT STRAND 54..57
FT /evidence="ECO:0007829|PDB:4XAX"
FT HELIX 59..62
FT /evidence="ECO:0007829|PDB:4XAX"
FT STRAND 64..73
FT /evidence="ECO:0007829|PDB:4XAX"
FT HELIX 80..84
FT /evidence="ECO:0007829|PDB:4XAX"
FT TURN 85..87
FT /evidence="ECO:0007829|PDB:4XAX"
FT STRAND 91..102
FT /evidence="ECO:0007829|PDB:4XAX"
FT TURN 103..105
FT /evidence="ECO:0007829|PDB:4XAX"
FT STRAND 108..119
FT /evidence="ECO:0007829|PDB:4XAX"
FT STRAND 123..125
FT /evidence="ECO:0007829|PDB:4XAX"
FT STRAND 127..129
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 132..136
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 138..142
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 144..146
FT /evidence="ECO:0007829|PDB:1YNJ"
FT HELIX 147..156
FT /evidence="ECO:0007829|PDB:4XAX"
FT STRAND 158..163
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 164..169
FT /evidence="ECO:0007829|PDB:1I6V"
FT STRAND 172..176
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 178..180
FT /evidence="ECO:0007829|PDB:1HQM"
FT STRAND 182..188
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 190..192
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 193..199
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 204..209
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 212..214
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 218..220
FT /evidence="ECO:0007829|PDB:5TJG"
FT TURN 224..228
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 231..242
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 243..245
FT /evidence="ECO:0007829|PDB:1YNJ"
FT HELIX 250..261
FT /evidence="ECO:0007829|PDB:5TJG"
FT TURN 264..266
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 271..281
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 286..294
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 297..302
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 303..315
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 316..318
FT /evidence="ECO:0007829|PDB:1I6V"
FT STRAND 330..334
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 336..356
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 357..359
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 360..362
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 364..366
FT /evidence="ECO:0007829|PDB:5TJG"
FT TURN 369..372
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 376..388
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 392..394
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 400..406
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 409..412
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 414..418
FT /evidence="ECO:0007829|PDB:1YNJ"
FT TURN 424..428
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 432..434
FT /evidence="ECO:0007829|PDB:5TJG"
FT TURN 435..437
FT /evidence="ECO:0007829|PDB:5TJG"
FT TURN 447..451
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 452..455
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 456..458
FT /evidence="ECO:0007829|PDB:1HQM"
FT STRAND 467..486
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 488..491
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 494..497
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 499..501
FT /evidence="ECO:0007829|PDB:1YNJ"
FT STRAND 503..508
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 511..519
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 521..524
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 526..528
FT /evidence="ECO:0007829|PDB:1YNJ"
FT STRAND 531..534
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 536..539
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 544..546
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 550..552
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 555..565
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 578..580
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 584..590
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 594..596
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 598..607
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 610..615
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 616..618
FT /evidence="ECO:0007829|PDB:1YNJ"
FT STRAND 620..624
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 628..630
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 634..637
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 650..652
FT /evidence="ECO:0007829|PDB:1HQM"
FT STRAND 654..657
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 659..662
FT /evidence="ECO:0007829|PDB:1YNJ"
FT STRAND 669..677
FT /evidence="ECO:0007829|PDB:5TJG"
FT TURN 680..683
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 684..686
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 688..691
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 692..696
FT /evidence="ECO:0007829|PDB:5TJG"
FT TURN 697..700
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 702..715
FT /evidence="ECO:0007829|PDB:5TJG"
FT TURN 727..730
FT /evidence="ECO:0007829|PDB:1I6V"
FT HELIX 732..734
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 741..743
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 754..756
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 758..761
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 769..778
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 784..787
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 798..807
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 808..810
FT /evidence="ECO:0007829|PDB:1YNJ"
FT STRAND 819..831
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 838..840
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 842..844
FT /evidence="ECO:0007829|PDB:1I6V"
FT STRAND 846..853
FT /evidence="ECO:0007829|PDB:5TJG"
FT TURN 855..857
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 868..871
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 873..875
FT /evidence="ECO:0007829|PDB:5TJG"
FT TURN 877..879
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 883..897
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 900..902
FT /evidence="ECO:0007829|PDB:5TJG"
FT TURN 905..907
FT /evidence="ECO:0007829|PDB:1YNJ"
FT HELIX 911..931
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 938..949
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 955..957
FT /evidence="ECO:0007829|PDB:1HQM"
FT HELIX 959..967
FT /evidence="ECO:0007829|PDB:5TJG"
FT TURN 968..970
FT /evidence="ECO:0007829|PDB:5TJG"
FT TURN 977..979
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 987..997
FT /evidence="ECO:0007829|PDB:5TJG"
FT TURN 998..1000
FT /evidence="ECO:0007829|PDB:1I6V"
FT HELIX 1001..1003
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 1006..1010
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 1013..1017
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 1024..1026
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 1030..1033
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 1034..1043
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 1046..1053
FT /evidence="ECO:0007829|PDB:5TJG"
FT TURN 1054..1058
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 1060..1071
FT /evidence="ECO:0007829|PDB:5TJG"
FT HELIX 1083..1093
FT /evidence="ECO:0007829|PDB:5TJG"
FT TURN 1094..1096
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 1097..1102
FT /evidence="ECO:0007829|PDB:5TJG"
FT STRAND 1106..1108
FT /evidence="ECO:0007829|PDB:1HQM"
FT STRAND 1111..1116
FT /evidence="ECO:0007829|PDB:5TJG"
SQ SEQUENCE 1119 AA; 124758 MW; 2CEF66FA79C77F33 CRC64;
MEIKRFGRIR EVIPLPPLTE IQVESYKKAL QADVPPEKRE NVGIQAAFKE TFPIEEGDKG
KGGLVLDFLE YRIGDPPFSQ DECREKDLTY QAPLYARLQL IHKDTGLIKE DEVFLGHLPL
MTEDGSFIIN GADRVIVSQI HRSPGVYFTP DPARPGRYIA SIIPLPKRGP WIDLEVEASG
VVTMKVNKRK FPLVLLLRVL GYDQETLVRE LSAYGDLVQG LLDEAVLAMR PEEAMVRLFT
LLRPGDPPKK DKALAYLFGL LADPKRYDLG EAGRYKAEEK LGVGLSGRTL VRFEDGEFKD
EVFLPTLRYL FALTAGVPGH EVDDIDHLGN RRIRTVGELM ADQFRVGLAR LARGVRERMV
MGSPDTLTPA KLVNSRPLEA ALREFFSRSQ LSQFKDETNP LSSLRHKRRI SALGPGGLTR
ERAGFDVRDV HRTHYGRICP VETPEGANIG LITSLAAYAR VDALGFIRTP YRRVKNGVVT
EEVVYMTASE EDRYTIAQAN TPLEGDRIAT DRVVARRRGE PVIVAPEEVE FMDVSPKQVF
SLNTNLIPFL EHDDANRALM GSNMQTQAVP LIRAQAPVVM TGLEERVVRD SLAALYAEED
GEVVKVDGTR IAVRYEDGRL VEHPLRRYAR SNQGTAFDQR PRVRVGQRVK KGDLLADGPA
SEEGFLALGQ NVLVAIMPFD GYNFEDAIVI SEELLKRDFY TSIHIERYEI EARDTKLGPE
RITRDIPHLS EAALRDLDEE GIVRIGAEVK PGDILVGRTS FKGEQEPSPE ERLLRSIFGE
KARDVKDTSL RVPPGEGGIV VGRLRLRRGD PGVELKPGVR EVVRVFVAQK RKLQVGDKLA
NRHGNKGVVA KILPVEDMPH LPDGTPVDVI LNPLGVPSRM NLGQILETHL GLAGYFLGQR
YISPVFDGAT EPEIKELLAE AFNLYFGKRQ GEGFGVDKRE KEVLARAEKL GLVSPGKSPE
EQLKELFDLG KVVLYDGRTG EPFEGPIVVG QMFIMKLYHM VEDKMHARST GPYSLITQQP
LGGKAQFGGQ RFGEMEVWAL EAYGAAHTLQ EMLTIKSDDI EGRNAAYQAI IKGEDVPEPS
VPESFRVLVK ELQALALDVQ TLDEKDNPVD IFEGLASKR
