RPOB_THEP1
ID RPOB_THEP1 Reviewed; 1263 AA.
AC A5IJW3;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2007, sequence version 1.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Tpet_0462;
OS Thermotoga petrophila (strain ATCC BAA-488 / DSM 13995 / JCM 10881 /
OS RKU-1).
OC Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX NCBI_TaxID=390874;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-488 / DSM 13995 / JCM 10881 / RKU-1;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C.,
RA Tapia R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P.;
RT "Complete sequence of Thermotoga petrophila RKU-1.";
RL Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000702; ABQ46486.1; -; Genomic_DNA.
DR RefSeq; WP_011943104.1; NC_009486.1.
DR AlphaFoldDB; A5IJW3; -.
DR SMR; A5IJW3; -.
DR STRING; 390874.Tpet_0462; -.
DR EnsemblBacteria; ABQ46486; ABQ46486; Tpet_0462.
DR KEGG; tpt:Tpet_0462; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_0; -.
DR OMA; FMTWEGY; -.
DR Proteomes; UP000006558; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1263
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000329195"
SQ SEQUENCE 1263 AA; 143205 MW; 61A5BD71D41798FE CRC64;
MKEISCGRRT RVSFGKTQEP LPIPDLVEIQ KISYRRFLEE GLLEVLKKFS PIYSQATRSD
LKKSDRGFAL EFVSTRIGEP VVDPLECKAK GLTYSVPIYA TARLTDMKSG EMKEEEVFLG
YIPYMTDRGT FIINGAERVV VNQIVVSPGL YFSSEYIDRE EYGGYFLPSR GAWLEVILDP
YDGVLYAGLD GKKVNLFLFL KTIGYEKDED ILSLYPTYLD ADDEDSLLLH VGSILLEDIY
DGDRKIAEKW DILTKDLAER ILMIDDINQI KIVHPIAQNT FEKMLELVSS SGEEGEEEEE
KTKIYGLNEV TVVDAYLEIF RRLRPEELPR INAAKRYLHD LFFNPERYDL SEVGRYKVNE
RLRNAYIRYL IEVEGEDPEE ARKKVYNENS LVLKPLDIVL ASRILFDYFE RRYVNDFEID
SYELRNLIRI FKEEYLEKRK TASYDLRKLV SVFRRNYGVT SDLSVLAAIR YISNINKELP
SIPFDTKDHL GNKRVRTVGE LVQREFERLF ARAQKAIQER LTLINSLSKV SIQSLINIKS
IISTVNQFFA MNQLSQFMDQ VNPLSELTHK RRVSAVGPGG LRRESKVFEA RNVHYSQYGR
LCPIETPEGA NIGFITSLAI YTKIDEYGFL MTPYRKVVNG KVTDEVVYLR ANEEEDFKIV
PATTPVDEEG NIIPERVVAR VGEDIRLVPR EEVDFMDVST KQPFSVSASL IPFLEHDDAS
RALMGSNMQR QAVPLLKAEA PFVGTGMEWE AAKNSGYVTL AEHDGIVKEV DAARIVVHRT
DENGNLMYDD KGNPVVDEYR LLKFVRSNQD TMINQKPIVN EGDFVKKGDP IADGPATDMG
ELALGRNILV AFMPWEGYNY EDAILVSQEL LEEDVFTSIH IEVYETQARE TRLGPEEITA
DIPNVSKELL KNLDENGIIR VGAYVVSDYG VGSQAILVGK VTPKGEGDTT PEEKIIRSVF
GERGRDVKDT SLRLPHGVEG RVIRVDVYDQ NDIAELGAGV LKLVRVYVAS RKTLDIGDKL
AGRHGNKGVV SNILPKEDMP FLPDGTPVQM VLNPLGIPSR MNVGQILETH LGWLAKLTGK
WFATPVFEGA KEDEILRPLY EERKKRGLHL GDDENNPNGK VVLRDGRTGE PFDNPVVVGY
MYMLKLVHIA KEKIHARSTG PYSLIHQQPL GGKSHFGGQR LGEMEVWALE AYGAAHTLAE
MLTIKSDDIK GRNEAYKAIL KNMNIPEPGV PESFRVLIKE LRGLALDVRL YDENGNEIDI
DKY
