RPOB_THEP3
ID RPOB_THEP3 Reviewed; 1238 AA.
AC B0KCJ2;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Teth39_0366;
OS Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium
OS thermohydrosulfuricum).
OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC Thermoanaerobacteraceae; Thermoanaerobacter.
OX NCBI_TaxID=340099;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33223 / DSM 2355 / 39E;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Bruce D., Goodwin L., Saunders E., Brettin T.,
RA Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L.,
RA Kyrpides N., Lykidis A., Hemme C., Fields M.W., He Z., Zhou J.,
RA Richardson P.;
RT "Complete sequence of Thermoanaerobacter pseudethanolicus 39E.";
RL Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000924; ABY94035.1; -; Genomic_DNA.
DR RefSeq; WP_012268991.1; NC_010321.1.
DR AlphaFoldDB; B0KCJ2; -.
DR SMR; B0KCJ2; -.
DR STRING; 340099.Teth39_0366; -.
DR PRIDE; B0KCJ2; -.
DR EnsemblBacteria; ABY94035; ABY94035; Teth39_0366.
DR KEGG; tpd:Teth39_0366; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_3_9; -.
DR OMA; FMTWEGY; -.
DR Proteomes; UP000002156; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1238
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000165830"
FT REGION 1187..1238
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1193..1238
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1238 AA; 138916 MW; A9845F2FF359843F CRC64;
MVRPVQVGNK TRMSFAKIDE VLEMPDLIEV QKKSYKWFLE EGLREVFREI SPIESFTGNL
ALEFVDYRLE NNPKYSVEEC KDRDTTYAVP MKVKVRLTNR ETGEIKESEV FMGDFPLMTE
KGTFIINGAE RVIVSQLVRS PGVYYEQQFD KFGKKLISAT VIPNRGAWLE YEEDSNDIVY
VRIDRTRKVP ITVLLRALGY STDIQILDLL GEEEKLKATL DKDTTKSEEE ALIEIYKRLR
PGEPPTVESA KSLLYALFFD AKRYDLAKVG RYKFNKKLAL KTRIANLKSA KKIVNPVTGE
ILVEEGEKIS KEKAEEIQNC GINVVEVLVE GKVVKVIGNN TVDINKYPMP YDVSSLNIKE
AVNLSVLKEI LDNFSDEEAV INEIKNRMDE LVPKHITKDD IIATISYQLN LTHGIGSIDD
IDHLGNRRLR SVGELLQNQF RIGLARLERV VKERMTIQDV NEITPQNLIN IRPVVAAIRE
FFGSSQLSQF MDQTNPLAEL THKRRVSALG PGGLSRERAG FEVRDVHYSH YGRICPIETP
EGPNIGLIGS LTTYARVNEY GFIEAPYRRV DKTTGTVTDE IVYMTADEED EYIIAQANEP
LDENNRFINE KVVCRLKEEI IAVPPTEVDF MDVSPKQIVS VATSMIPFLE NDDANRALMG
SNMQRQAVPL IKPEAPIIGT GIEYKAAVDS GVVVLAKNDG VVEKVAADKV VIRTKDGRRD
EYNLLKFKRS NQGTCINQRP IVNEGDEVKK GQVICDGPST DYGELALGKN VLVGFMPWEG
YNYEDAILIS EELVRDDSLT SIHIEEYDAE ARDTKLGPEE ITREIPNVGE DALKDLDERG
IIRIGAEVTA GDILVGKVTP KGETELTAEE RLLRAIFGEK AREVRDTSLR VPHGESGIVV
DVKVYSRENG DELPPGVNQM VRVFVAQKRK ISVGDKMAGR HGNKGVISRI LPVEDMPFLP
DGTPLQICLN PLGVPSRMNI GQVLEVHLGL VAKALGWQIA TPVFDGATEE DIQELLAKSG
FSPDGKVQLY DGRTGEPFDN KVTVGYMYML KLHHLVDDKM HARSTGPYSL VTQQPLGGKA
QFGGQRFGEM EVWALEAYGA AHTLQEILTV KSDDVSGRVK TYEAIVKGEN IPEPGIPESF
KVLVKELQSL ALDVKVITED NQEIPLKEFE DDDDSDVPDA TLNINIEGRE DTPPEEVYEE
SYEEGFEEEI EELPEDIDFE PDSFDIENDD LDLEDFDI
