RPOB_TREPA
ID RPOB_TREPA Reviewed; 1178 AA.
AC O83269;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=TP_0241;
OS Treponema pallidum (strain Nichols).
OC Bacteria; Spirochaetes; Spirochaetales; Treponemataceae; Treponema.
OX NCBI_TaxID=243276;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Nichols;
RX PubMed=9665876; DOI=10.1126/science.281.5375.375;
RA Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G.,
RA Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A.,
RA Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D.,
RA Khalak H.G., Richardson D.L., Howell J.K., Chidambaram M., Utterback T.R.,
RA McDonald L.A., Artiach P., Bowman C., Cotton M.D., Fujii C., Garland S.A.,
RA Hatch B., Horst K., Roberts K.M., Sandusky M., Weidman J.F., Smith H.O.,
RA Venter J.C.;
RT "Complete genome sequence of Treponema pallidum, the syphilis spirochete.";
RL Science 281:375-388(1998).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE000520; AAC65229.1; -; Genomic_DNA.
DR PIR; C71350; C71350.
DR RefSeq; WP_010881689.1; NC_021490.2.
DR AlphaFoldDB; O83269; -.
DR SMR; O83269; -.
DR STRING; 243276.TPANIC_0241; -.
DR EnsemblBacteria; AAC65229; AAC65229; TP_0241.
DR KEGG; tpa:TP_0241; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_12; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000000811; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1178
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000047987"
SQ SEQUENCE 1178 AA; 132447 MW; E429EF184B5DB997 CRC64;
MSARVCKTHR VYVGRDVRNF MDIPDLIEIQ LRSYDTFLHG ARNTPSGADT LISGTREELG
LEDVFKTTFP IESSTGDMTL EYQSYSLDEK NIKFSEAECK QKGLTYAIPL KALVDLRFNN
TGEIRRKDIY MGDIPKMTER GTFIINGAER VVVSQIHRSP GVVFSHEKDK EGREVFSSRI
IPYRGSWLEF EIDQKKDLIY AKLDKKRRIL GTVFLRALHY ETREQIIEAF YAIEKTPVCQ
DRAEYELLTG KILARSVTVE NEQGETRVLY KAGEKIHPHV IDDLLQNGIC EVYIINLEAE
GSLRSAVVIN CLEREEMKFS KSGAQDELSR EEALCIVYSA LRPSDPMTMD AAEKDLQTMF
FSPRRYDLGR VGRYKLNKKF RSDSPTTECT LTLDDIVNTM KFLIRMYSGD AQEDDIDHLG
NRRIRSVGEL MTNTLKTAFL RMERIAKERM SSKETETIKP QDLISIKPIM AAIKEFFGAS
QLSQFMDQVN PLAELTHKRR LNALGPGGLS RERAGFEVRD VHYTHYGRMC PIETPEGPNI
GLIVSMANYA RVNGYGFLEV PYVRVRDGVV TKEIEYLDAM DEDRYYIGQD STAVGPDGVI
RVDHVSCRHR GDYSTRSPKD IQYMDVSPKQ IISVSASLIP FLEHDDANRA LMGSNMQRQG
VPLIFPEPPR VGTGMEEKCA YDSGVLVKAK QDGTVAYVSS EKIVVCSAAA SGEEQEVVYP
LLKYQRTNQD TCYHQRPIVH VGDRVQVGDA LADGPATYRG ELALGRNILV GFVPWNGYNY
EDAILISHRV VKEDMFTSVH IKEFSTEVRE TKLGSERMTN DIPNKSEKNL DNLDAEGIIR
IGSKVRAGDV LIGKITPKSE SETTPEFRLL NSIFGEKAKE VRDSSLRVPH GVEGTVIDVQ
RLRRSEGDDL NPGVSEVVKV LIATKRKLRE GDKMAGRHGN KGIVARILPE EDMPYLDDGT
PLDVCLNPLG VPSRMNIGQI LESELGLAGL RLDEWYESPV FQSPSNEQIG EKLMQAGFPT
NSKVMLRDGR TGDYFQNPVF VGVIYFMKLA HLVDDKMHAR STGPYSLVTQ QPLGGKAQFG
GQRLGEMEVW ALEAYGAANT LQELLTIKSD DMHGRSKIYE AIVKGEASSP TGIPESFNVL
VQELRGLALD FTIYDAKGKQ IPLTERDEEM TNKIGSKF
