ID   RPOB_TUPAK              Reviewed;        2178 AA.
AC   Q3ZJ93;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2005, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS   Tupiella akineta (Green alga) (Pseudendoclonium akinetum).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; Ulvophyceae; OUU clade;
OC   Ulotrichales; Tupiellaceae; Tupiella.
OX   NCBI_TaxID=160070;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UTEX 1912;
RX   PubMed=15930151; DOI=10.1093/molbev/msi182;
RA   Pombert J.-F., Otis C., Lemieux C., Turmel M.;
RT   "The chloroplast genome sequence of the green alga Pseudendoclonium
RT   akinetum (Ulvophyceae) reveals unusual structural features and new insights
RT   into the branching order of chlorophyte lineages.";
RL   Mol. Biol. Evol. 22:1903-1918(2005).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AY835431; AAV80598.1; -; Genomic_DNA.
DR   RefSeq; YP_636174.1; NC_008114.1.
DR   AlphaFoldDB; Q3ZJ93; -.
DR   SMR; Q3ZJ93; -.
DR   GeneID; 4108778; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 2.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 2.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 3.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..2178
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000224132"
FT   REGION          269..325
FT                   /note="Insert"
FT   REGION          714..1508
FT                   /note="Insert"
FT   REGION          1703..1900
FT                   /note="Insert"
SQ   SEQUENCE   2178 AA;  252534 MW;  3B358C86271E9F02 CRC64;
     MQTTQNRLKT KRYASFFLNP KLNRPIQKKG WIKKADFNQL TDSLCKQSLK AYNEKLYSPL
     SQFKIPDFLN IQRSSFRQFL KSGLITEFKN CKSITNSNQT FEVFFYAEHY KLNRPKWTPK
     QAILKKKSYS SQLYLPIQLS NYQTEEVHLQ WVLLANLPLM TKNGHFILNG CPRVLMNQIV
     RSPGVYFRKV VKNQKTVYSA DFIAQRGSWL RLEVDTKKGD IWAKLKKTPK IPIFTFLRAL
     GLTLPILNNS IDFAKLRYLL QFKEESKRSK KIKDFLGPGI NNSLDIVEFR SLLKSLKKYM
     KPKPAKKSSK KQKTKKIKKW LTPFVCTTYF QSLLELCQKL YPQKKFLELD PKLAQKFLFR
     KFQNPRTYDL SILGREKINK KLGISIPLEK TILTSQDVLF ACLFLMDLLQ GLVISDDIDD
     LKNRKIKPSG ELIQTQLSTG LIRLEKIIRE KLKQPKQDSD SFSNLFSVKP LNQAFRDFFG
     TNPLSQLMDQ TNALSEITHK RRLSSLGPGG INRETAGMAI RGIHPTHYGR ICPIETPEGQ
     NAGLVNSFTI FSHLNTKSFI ETPFYKMYKG FILKNKNPFL FSSDQERNLV LAPGDIQTSL
     FHFLPPGVPI PSRQFKEFKR VSRNEINFIA VSPIQMISIA TSLIPFLEHN DGNRALMGSN
     MQRQAVPTVR PSKPIVGTGL ESRVISDVGH GLQVQKSGFV SYVDGNKIII YSKKRIDQPF
     FFDTKKLHFP KKKVPFLNLD LQQQNTCRSS FKKKLVKSAN IFTFKEKQLY QLSKIKNKKQ
     RVCFEKSLKS QDFSSLLNFL TLFNIKTKVQ AEKKASVQDA SFILVPKKNL DLEVNKRIFL
     PVFLKKRNEY NFLNYYKLTE RTLYSGFSIL PFKLTNKGNY FNNKQISCLS NSLFLNDFLG
     QFHLLKFFST KIFYSKLSRN FKPFISKRSL VLINKFSHQK LLFSYCKIIL IHFYTKFNKK
     TPKNFLLNTK LSSKLMKRKD FSHFFRKDEK TMPIQNLTNF KSAHLHSQTA VKGVCQQIRS
     ARKTQIANPF WYNSFPLFLI KSSPLTPLLF RRSFVPKVFN PLGLHFAYQQ SKGPKHVSRT
     FKTLDRNEQK FSYNQKFQKL NFIFVKPYKF KRKNSPFAPF ARSSKARGSA KAIFSQAQRL
     WGEKVFLVQN KENLPQTLGS CKIMTNQFYL KFSLLLKQQK KNFSNKKTGM ENQNCQNSLT
     SQKNGFQCLK KMEPLHFPTK KSSISTLSFV KKILPYFQHP LLNLTTQNKI KNKLMIFKKP
     FLFWSACFAD KQSLRSILFS LKKSQKVTTL KKTKVKKKFE IVYNENKKKY FQKINKNFFL
     PLLCRTRNLN FASNSLLFQI SYPVAVNQSN LIQRNLISQE LKHNSKRFIK TLLKIKPFVG
     KFVFPFCPIC LKRKASIETQ SFVKKSDKNF QFCFCFSRKK QKTLKPLLRI KSSDKMNTLK
     PLQKFSLLEN FKVPPQSSLT ARDKILRSTI GTKGLNDSLS MKSTDGEYAI NDKKRRVEII
     NSSSLFYNNL IPQNYILNNY HRSNQDTYMI HRPLVQEGQW VEKGDILADS SVSVQGELSI
     GQNILVGYIP WEGYNFEDAV LISERLIFDD LYTSLHIERY EVEIRDTQFG LEQITNQIPN
     EKGEYDYIDS NGIAKIGTWV KEGDILVGKI APIGQKKLTA YENLLYDILN KEIPKTRDTS
     LRVPRGVTGR VIHVEIVETT KKKGNDSSIF AKSLKFPKKQ KLKRVTSPEY WYPVNTKKDQ
     KVQSFSFKNK SEKLFLTPVQ SYFNKEAKTK NPKLNSPFFY LNKRNPDFNL KRNKRIAKEM
     YFFNKNEKIK LSTLQIKIKG LEKKEFSFPK ILKARFSLLQ KAQTANLKIE KFLDDKFWNN
     VNYSNKNFSR NGSQKENSNA DGLQFYNPLK KTKTKVLQPM EKTSFSGPQK VHIYLAEKRK
     LQIGDKIAGR HGNKGIISNI LPRQDMPYLS DGTPLDIVLN PLGVPSRMNV GQILECLLGM
     AGYYLKQNFK IQPFDEIYGC EASRSLVYSK LYEARIKTGQ DWLFNANHPG KTRLFDGRTG
     DCFKQPVTVG VAYILKLIHL VDDKIHARST GPYSLVTQQP LRGRSKQGGQ RVGEMEVWAL
     EGFGAAYILQ EILTIKSDDL KGRNQVMNSI LNNKPVNFGL PESFKVLVRE LQSLCLDIAI
     YRYRENGTPT KININNFW