RPOB_VEREI
ID RPOB_VEREI Reviewed; 1370 AA.
AC A1WK55;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Veis_2264;
OS Verminephrobacter eiseniae (strain EF01-2).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Verminephrobacter.
OX NCBI_TaxID=391735;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EF01-2;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T.,
RA Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P.;
RT "Complete sequence of chromosome 1 of Verminephrobacter eiseniae EF01-2.";
RL Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000542; ABM58012.1; -; Genomic_DNA.
DR RefSeq; WP_011810015.1; NC_008786.1.
DR AlphaFoldDB; A1WK55; -.
DR SMR; A1WK55; -.
DR STRING; 391735.Veis_2264; -.
DR PRIDE; A1WK55; -.
DR EnsemblBacteria; ABM58012; ABM58012; Veis_2264.
DR KEGG; vei:Veis_2264; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_3_4; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000000374; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1370
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300423"
SQ SEQUENCE 1370 AA; 153030 MW; 704E8F8DD816FF3B CRC64;
MAYSFTERKR IRKSFGTRDS VLEVPYLLQM QKDAYTAFLQ ADKEPRKRTI EGLQAAFDAA
FPIVSHNGFV EMKFIEYNLA RPAFDVRECQ TRGLTFASAV RAKVQLIIYD RESSTSQSKV
VKEVKEQEVY MGEVPLMTDK GSFIINGTER VIVSQLHRSP GVFFEHDKGK THGSGKLLFS
ARIIPYRGSW LDFEFDPKDI LYFRVDRRRK MPVTILLKAI GLNPESILAN FFVNDNFRLM
DSGAQMEFVP ERLRGEVARF DITDKSGKLI VAKDKRVTAR HTRDLEQSGS THISVPEDFL
VGRVVARTIV DADSGEILAK ANDELTEALL KKLRSAAVRE LQCIYTNELD QGAYISHTLR
SDETVDEFAA RVAIYRMMRP GEPPTEDAVQ ALFQRLFYNP DTYDLSRVGR MKFNARIGRD
ESTGPMVLSN EDILAVVKIL VDLRNGNGEV DDIDHLGNRR VRCVGELAEN QYRTGLARIE
KAVKERLGQA EQEPLMPHDL INSKPISAAL KEFFGASQLS QFMDQTNPLA EITHKRRVSA
LGPGGLTRER AGFEVRDVHV THYGRVCPIE TPEGPNIGLI NSLALYARLN EYGFIETPYR
RVVGGMVTND IDYLSAIEEG KYVIAQANAV LDKEGRLTGD LVSAREKGES ILCAADRVRY
MDVSPAQIVS VAASLVPFLE HDDANRALMG ANMSRQAVPV LRPEKPLVGT GIERVAAIDS
GTVVTATRGG SVDYVDATRI VVRVNDDETV AGEVGVDIYN LIKYQRSNQN TNIHQRPIVK
KGDRLVKGDV IADGASTDLG EIAIGQNMLI AFMPWNGYNF EDSILISERV VSEDRYTSIH
IEELVVMARD TKLGAEEITR DIPNLSEQQL NRLDESGIIY VGAEVQPGDT LVGKVTPKGE
TTLTPEEKLL RAIFGEKASD VKDTSLRVEQ GSQGTVIDVQ VFTREGIARD KRAQQIIDDE
LKRFRLDLND QLRIVEADAF ERIEKLLLGR VANGGPHKLL KGAKIDKPYL SSVEKFHWFD
IRPAQDEVAA QLELIKDALE QTRHSFDLAF EEKKKKLTQG DELPAGVLKM VKVYLAVKRR
LQPGDKMAGR HGNKGVVSKI VPVEDMPYMA DGTPADIVLN PLGVPSRMNI GQVLEVHLGW
ASKGIGQRIG DMLQQQAKAA ELRKFLDKVY NARGRTEDLA QLCDEELVAM AAHLRHGMPY
ATPVFDGASE EEIKDMLKIA YPDEIAQRKG LTATRTQAFL YDGRTGERFE RPTTIGYMHY
LKLHHLVDDK MHARSTGPYS LVTQQPLGGK AQFGGQRFGE MEVWALEAYG AAYVLQEMLT
VKSDDVAGRT KVYESIVKGE HAIEAGMPES FNVLVKEIRS LGLDIELERS
