ID   RPOB_VIBC3              Reviewed;        1341 AA.
AC   A5F3P5; C3M3Y6;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 2.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
GN   OrderedLocusNames=VC0395_A2730, VC395_0371;
OS   Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS   O395).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=345073;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA   Heidelberg J.;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX   PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA   Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA   Wang W., Wang J., Qian W., Li D., Wang L.;
RT   "A recalibrated molecular clock and independent origins for the cholera
RT   pandemic clones.";
RL   PLoS ONE 3:E4053-E4053(2008).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABQ20577.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=ACP08394.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000627; ABQ20577.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP001235; ACP08394.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_000263119.1; NZ_CP045719.1.
DR   AlphaFoldDB; A5F3P5; -.
DR   SMR; A5F3P5; -.
DR   STRING; 345073.VC395_0371; -.
DR   PRIDE; A5F3P5; -.
DR   EnsemblBacteria; ABQ20577; ABQ20577; VC0395_A2730.
DR   KEGG; vco:VC0395_A2730; -.
DR   KEGG; vcr:VC395_0371; -.
DR   PATRIC; fig|345073.21.peg.359; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_3_6; -.
DR   Proteomes; UP000000249; Chromosome 2.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase.
FT   CHAIN           1..1341
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000329196"
SQ   SEQUENCE   1341 AA;  149513 MW;  B6E0E643FB61BC4B CRC64;
     MVYSYTEKKR IRKDFGTRPQ VLDIPYLLSI QLDSFEKFIE QDPEGQYGLE AAFRSVFPIQ
     SYNGNSELQY VSYRLGEPVF DVKECQIRGV TYSKPLRVKL RLVIFDKDAP AGTVKDIKEQ
     EVYMGEIPLM TENGTFVING TERVIVSQLH RSPGVFFDSD KGKTHSSGKV LYNARIIPYR
     GSWLDFEFDP KDNLYVRIDR RRKLPASIIL RVLGKTSAEI LDIFFEKVNF EVKDQTLMME
     LVPERLRGET ATFDIEADGK VYVEKGRRVT ARHIRQLEKD GVNFIEVPVE YIVGKVSAKD
     YVNEATGELI ITANQEISLE ALANLSQAGY KKLEVLFTND LDHGPFMSET LRVDSTTDRI
     SALVEIYRMM RPGEPPTKEA AESLFESLFF SAERYDLSTV GRMKFNSSIG REDAEEQGTL
     DEVDIIEVMK KLISIRNGKG EVDDIDHLGN RRIRSVGEMA ENQFRVGLVR VERAVKERLS
     LGDLDNVMPQ DLINAKPISA AVKEFFGSSQ LSQFMDQNNP LSEVTHKRRI SALGPGGLTR
     ERAGFEVRDV HVTHYGRLCP IETPEGPNIG LINSLSAFAR CNEYGFLETP YRRVVNGVVT
     DEVDYLSAIE EGQFVIAQAN AKLTEEGGFA DELVTARQKG ESGLHPREHV DYMDVATNQV
     VSIAASLIPF LEHDDANRAL MGANMQRQAV PTLRSEKPLV GTGIERNVAV DSGVTAVAKR
     GGVIQSVDAS RIVVKVNEEE LIPGEAGIDI YNLTKYTRSN QNTCINQRPC VMPGEPVARG
     DVLADGPSTD LGELALGQNM RIAFMPWNGY NFEDSILVSE RVVQDDRFTT IHIQELSCVA
     RDTKLGAEEI TADIPNVGEA ALSKLDESGI VYIGAEVKGG DILVGKVTPK GETQLTPEEK
     LLRAIFGEKA SDVKDTSLRV PNSVAGTVID VQVFTRDGVE KDKRALEIEQ MQLKEAKKDL
     TEEFQILEGG LLARVRSVLL AGGYTEAKLS SIERKKWLEQ TLENEELQNQ LEQLAEQYDE
     LKADFDKKFE AKRRKITQGD DLAPGVLKIV KVYLAVKRRI QPGDKMAGRH GNKGVISKIN
     PVEDMPYDEN GQPVDIVLNP LGVPSRMNIG QILEVHLGLA AKGIGDKINQ MIKEQQELAK
     LREFLQKVYD LGDTRQRVDI SELSDEDVRT LAHNLRAGLP VATPVFDGAP ESSIKAMLEL
     ADLPASGQLT LFDGRTGDAF ERPVTVGYMY MLKLNHLVDD KMHARSTGSY SLVTQQPLGG
     KAQFGGQRFG EMEVWALEAY GAAYTLQEML TVKSDDVNGR TKMYKNIVDG NHAMEPGMPE
     SFNVLLKEIR SLGINIELED E