RPOB_VIBPA
ID RPOB_VIBPA Reviewed; 1342 AA.
AC Q87KQ4;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=VP2922;
OS Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=223926;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RIMD 2210633;
RX PubMed=12620739; DOI=10.1016/s0140-6736(03)12659-1;
RA Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT distinct from that of V. cholerae.";
RL Lancet 361:743-749(2003).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; BA000031; BAC61185.1; -; Genomic_DNA.
DR RefSeq; NP_799301.1; NC_004603.1.
DR RefSeq; WP_005456486.1; NC_004603.1.
DR AlphaFoldDB; Q87KQ4; -.
DR SMR; Q87KQ4; -.
DR STRING; 223926.28807948; -.
DR EnsemblBacteria; BAC61185; BAC61185; BAC61185.
DR GeneID; 1190497; -.
DR KEGG; vpa:VP2922; -.
DR PATRIC; fig|223926.6.peg.2810; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_6; -.
DR OMA; FMTWEGY; -.
DR Proteomes; UP000002493; Chromosome 1.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1342
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000047993"
SQ SEQUENCE 1342 AA; 149553 MW; D225B51A768DDD6A CRC64;
MVYSYTEKKR IRKDFGTRPQ VLDIPYLLSI QLDSFDKFIE QDPEGQYGLE AAFRSVFPIQ
SYNGNSELQY VSYRLGEPVF DVKECQIRGV TYSKPLRVKL RLVIFDKDAP AGTVKDIKEQ
EVYMGEIPLM TDNGTFVING TERVIVSQLH RSPGVFFDSD KGKTHSSGKV LYNARVIPYR
GSWLDFEFDP KDNLYVRIDR RRKLPASIIL RALGKSTEEI LDIFFEKVNF EVKDQTLLME
LVPDRLRGET ASFDIESNGK VYVEQGRRVT ARHIRQLEKD GVDHIEVPVE YIVGKVASKD
YINEATGEII VNANQEISLE ALANLSQAGH KALEVLFTND LDHGPFMSET LRIDSTVDRI
SALVEIYRMM RPGEPPTKEA AEALFESLFF SEERYDLSTV GRMKFNSSIG REDAQEQGTL
DETDIIEVMK KLIAIRNGKG EVDDIDHLGN RRIRSVGEMA ENQFRVGLVR VERAVKERLS
LGDLDAVMPQ DLINAKPISA AVKEFFGSSQ LSQFMDQNNP LSEVTHKRRI SALGPGGLTR
ERAGFEVRDV HVTHYGRLCP IETPEGPNIG LINSLSAFAR CNEYGFLETP YRRVVDGVVT
DEVDYLSAIE EGQFVIAQAN AKLNEDGTFA DELITARQKG ESGLHPREHA QYMDVATNQV
VSIAASLIPF LEHDDANRAL MGANMQRQAV PTLKADKPLV GTGIERNVAV DSGVTAVAKR
GGVIQSVDAS RIVVKVNEEE LVPGEAGIDI YNLTKYTRSN QNTCINQRPC VMPGEPVARG
DVLADGPSTD LGELALGQNM RIAFMPWNGY NFEDSILVSE RVVQEDRFTT IHIQELTCVA
RDTKLGSEEI TADIPNVGES ALSKLDESGI VYIGAEVKGG DILVGKVTPK GETQLTPEEK
LLRAIFGEKA SDVKDTSLRV PNSVSGTIID VQVFTRDGVE KDKRALEIEQ MQLKEAKKDL
TEEFQILEGG LLNRVKAVLI EGGYSEAKLD ATDRKKWLEL TLEDDALQTQ LEQLAEQWDE
LKADFDKKFE TKRRKITQGD DLAPGVLKIV KVYLAVKRRI QPGDKMAGRH GNKGVISKIN
PVEDMPYDEK GQPVDIVLNP LGVPSRMNIG QILEVHLGLA AKGIGDKINQ MVKEQQELAK
FREFLQKVYD LGDTRQKVDI ASLSDDEVRT LIKNLRGGLP IATPVFDGAP EASIKALLEL
ADLPTSGQLT LFDGRTGDAF ERPVTVGYMY MLKLNHLVDD KMHARSTGSY SLVTQQPLGG
KAQFGGQRFG EMEVWALEAY GAAYTLQEML TVKSDDVNGR TKMYKNIVDG NHSMEPGMPE
SFNVLLKEIR SLGINIELED EE
