ID   RPOB_VIBVU              Reviewed;        1342 AA.
AC   Q8DD20;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=VV1_1211;
OS   Vibrio vulnificus (strain CMCP6).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=216895;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CMCP6;
RA   Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.;
RT   "Complete genome sequence of Vibrio vulnificus CMCP6.";
RL   Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AE016795; AAO09671.1; -; Genomic_DNA.
DR   RefSeq; WP_011079201.1; NC_004459.3.
DR   AlphaFoldDB; Q8DD20; -.
DR   SMR; Q8DD20; -.
DR   EnsemblBacteria; AAO09671; AAO09671; VV1_1211.
DR   KEGG; vvu:VV1_1211; -.
DR   HOGENOM; CLU_000524_4_3_6; -.
DR   OMA; FMTWEGY; -.
DR   Proteomes; UP000002275; Chromosome 1.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase.
FT   CHAIN           1..1342
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000047994"
SQ   SEQUENCE   1342 AA;  149619 MW;  33D873C489AFC126 CRC64;
     MVYSYTEKKR IRKDFGTRPQ VLDIPYLLSI QLDSFDKFIE QDPEGQYGLE AAFRSVFPIQ
     SYNGNSELQY VSYRLGEPVF DVKECQIRGV TYSKPLRVKL RLVIFDKDAP AGTVKDIKEQ
     EVYMGEIPLM TDNGTFVING TERVIVSQLH RSPGVFFDSD KGKTHSSGKV LYNARIIPYR
     GSWLDFEFDP KDNLYVRIDR RRKLPSTIIL RALGKSTEEI LDTFFEKVNF EVKDQTLMME
     LVPDRLRGET ATFDIEANGT VYVEKGRRVT ARHIRQLEKE GVDQIEVPVE YIVGKVSSKD
     YINEATGEII VAANQEISLE ALAKLSQAGH KQLEVLFTND LDHGPFMSET LRIDSSVDRI
     SALVEIYRMM RPGEPPTKEA AEALFESLFF SEERYDLSTV GRMKFNSSIG RDDAEEQGTL
     DETDIIEVMK KLIAIRNGKG EVDDIDHLGN RRIRSVGEMA ENQFRVGLVR VERAVKERLS
     LGDLDAVMPQ DLINAKPISA AVKEFFGSSQ LSQFMDQNNP LSEVTHKRRI SALGPGGLTR
     ERAGFEVRDV HVTHYGRLCP IETPEGPNIG LINSLSAFAR CNEYGFLETP YRRVVDGVVT
     DEVDYLSAIE EGQFVIAQAN AKLNEDGTFA DELITARQKG ESGLHPREHV DYMDVATNQV
     VSIAASLIPF LEHDDANRAL MGANMQRQAV PTLKAEKPLV GTGIERNVAV DSGVTSVAKR
     GGIIQSVDAS RIVVKVNEEE LIPGEAGIDI YNLTKYTRSN QNTCINQRPC VMPGEPVLRG
     DVLADGPSTD LGELALGQNM RIAFMPWNGY NFEDSILVSE RVVQEDRFTT IHIQELTCVA
     RDTKLGSEEI TADIPNVGES ALSKLDESGI VYIGAEVKGG DILVGKVTPK GETQLTPEEK
     LLRAIFGEKA SDVKDTSLRV PNSVSGTIID VQVFTRDGVE KDKRALEIEQ MQLKEAKKDL
     TEEFQILEGG LLNRVKAVLL SGGYSEAKLD TTDRKKWLEL TLEDDALQTQ LEQLAEQYDE
     LKADFDKKFE TKRRKITQGD DLAPGVLKIV KVYLAVKRRI QPGDKMAGRH GNKGVISKIN
     PVEDMPYDEK GQPVDIVLNP LGVPSRMNIG QILEVHLGLA AKGIGDKINQ MVKEQQELAK
     FREFLQKVYD LGETRQKVDI ASLSDEEVRT LIGNLRGGLP IATPVFDGAS EASIKELLKL
     GGLPESGQLT LFDGRTGDAF ERPVTVGYMY MLKLNHLVDD KMHARSTGSY SLVTQQPLGG
     KAQFGGQRFG EMEVWALEAY GAAYTLQEML TVKSDDVNGR TKMYKNIVDG NHAMEPGMPE
     SFNVLLKEIR SLGINIELED EQ