RPOB_WHEAT
ID RPOB_WHEAT Reviewed; 1076 AA.
AC Q9XPS7;
DT 14-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS Triticum aestivum (Wheat).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Chinese Spring;
RA Matsuoka Y., Tsunewaki K., Ohnishi Y.;
RT "Molecular analysis of a 21.1-kb fragment of wheat chloroplast DNA bearing
RT RNA polymerase subunit (rpo) genes.";
RL Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring;
RA Ogihara Y., Isono K., Kojima T., Endo A., Hanaoka M., Shiina T.,
RA Terachi T., Utsugi S., Murata M., Mori N., Takumi S., Ikeo K., Gojobori T.,
RA Murai R., Murai K., Matsuoka Y., Ohnishi Y., Tajiri H., Tsunewaki K.;
RT "Chinese spring wheat (Triticum aestivum L.) chloroplast genome: complete
RT sequence and contig clones.";
RL Plant Mol. Biol. Rep. 18:243-253(2000).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; AB027572; BAA78040.1; -; Genomic_DNA.
DR EMBL; AB042240; BAB47024.1; -; Genomic_DNA.
DR RefSeq; NP_114249.1; NC_002762.1.
DR AlphaFoldDB; Q9XPS7; -.
DR SMR; Q9XPS7; -.
DR STRING; 4565.EPlTAEP00000010068; -.
DR PRIDE; Q9XPS7; -.
DR EnsemblPlants; TraesPAR_scaffold_034002_01G000100.1; TraesPAR_scaffold_034002_01G000100.1; TraesPAR_scaffold_034002_01G000100.
DR EnsemblPlants; TraesPAR_scaffold_313113_01G000100.1; TraesPAR_scaffold_313113_01G000100.1; TraesPAR_scaffold_313113_01G000100.
DR GeneID; 803173; -.
DR Gramene; TraesPAR_scaffold_034002_01G000100.1; TraesPAR_scaffold_034002_01G000100.1; TraesPAR_scaffold_034002_01G000100.
DR Gramene; TraesPAR_scaffold_313113_01G000100.1; TraesPAR_scaffold_313113_01G000100.1; TraesPAR_scaffold_313113_01G000100.
DR KEGG; taes:803173; -.
DR eggNOG; KOG0214; Eukaryota.
DR Proteomes; UP000019116; Chloroplast.
DR ExpressionAtlas; Q9XPS7; baseline.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 3.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW Reference proteome; Transcription; Transferase.
FT CHAIN 1..1076
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000048052"
SQ SEQUENCE 1076 AA; 121651 MW; 58A9E387EEA0AD2F CRC64;
MLRNGNEGMS TIPGFSQIQF EGFCRFINQA LAEELDKFPT IKDPDHEIAF QLFAKGYQLL
EPSIKERDAV YESLTYSSEL YVSARLIFGF DVQKQTISIG NIPIMNSLGT FIINGIYRIV
INQILLSPGI YYRSELDHKG ISIYTGTIIS DWGGRSELAI DKKERIWARV SRKQKISILV
LSSAMGSNLR EILDNVSYPE IFLSFPNAKE KKRIESKEKA ILEFYQQFAC VGGDLVFSES
LCEELQKKFF QQKCELGRIG RRNMNRRLNL DIPQNNTFLL PRDVLAATDH LIGMKFGTGI
LDDDDMNHLK NKRIRSVADL LQDQFGLALG RLQHAVQKTI RRVFIRQSKP TPQTLVTPTS
TSILLITTYE TFFGTYPLSQ VFDQTNPLTQ TVHGRKVSCL GPGGLTGRTA SFRSRDIHPS
HYGRICPIDT SEGINVGLTG SLAIHARIDH LWGSIESPFY EISAEKAKEK KERQVVYLSP
NRDEYYMIAA GNSLSLNQGI QEEQVVPARY RQEFLTIAWE QIHVRSIFPF QYFSIGGSLI
PFIEHNDANR ALMSSNMQRQ AVPLSRSEKC IVGTGLERQT ALDSRVSIIA EREGKIISTD
SHKILLSSSG KTISIPLVNH RRSNKNTCMH QKPRVPRGKS IKKGQILAEG AATVGGELAL
GKNVLVAYMP WEGYNFEDAV LISERLVYED IYTSFHIRKY EIQTDTTSQG SAEKITKEIP
HLEEHLLRNL DRNGVVRLGS WVETGDILVG KLTPQIASES SYIAEAGLLR AIFGLEVSTS
KETSLKLPIG GRGRVIDVKW IQRDPLDIMV RVYILQKREI KVGDKVAGRH GNKGIISKIL
PRQDMPYLQD GTPVDMVFNP LGVPSRMNVG QIFESSLGLA GDLLKKHYRI APFDERYEQE
ASRKLVFSEL YEASKETKNP WVFEPEYPGK SRIFDGRTGD PFEQPVLIGK SYILKLIHQV
DEKIHGRSTG PYSLVTQQPV RGRAKQGGQR VGEMEVWALE GFGVAHILQE ILTYKSDHLI
ARQEILNATI WGKRIPNHED PPESFRVLVR ELRSLALELN HFLVSEKNFQ VNREEV
