RPOB_XANOP
ID RPOB_XANOP Reviewed; 1383 AA.
AC B2SQQ1;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-2008, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=PXO_04530;
OS Xanthomonas oryzae pv. oryzae (strain PXO99A).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Xanthomonas.
OX NCBI_TaxID=360094;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PXO99A;
RX PubMed=18452608; DOI=10.1186/1471-2164-9-204;
RA Salzberg S.L., Sommer D.D., Schatz M.C., Phillippy A.M., Rabinowicz P.D.,
RA Tsuge S., Furutani A., Ochiai H., Delcher A.L., Kelley D., Madupu R.,
RA Puiu D., Radune D., Shumway M., Trapnell C., Aparna G., Jha G., Pandey A.,
RA Patil P.B., Ishihara H., Meyer D.F., Szurek B., Verdier V., Koebnik R.,
RA Dow J.M., Ryan R.P., Hirata H., Tsuyumu S., Won Lee S., Seo Y.-S.,
RA Sriariyanum M., Ronald P.C., Sonti R.V., Van Sluys M.-A., Leach J.E.,
RA White F.F., Bogdanove A.J.;
RT "Genome sequence and rapid evolution of the rice pathogen Xanthomonas
RT oryzae pv. oryzae PXO99A.";
RL BMC Genomics 9:204-204(2008).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000967; ACD57878.1; -; Genomic_DNA.
DR RefSeq; WP_011260034.1; NC_010717.2.
DR PDB; 6J9E; EM; 3.41 A; C=1-1383.
DR PDBsum; 6J9E; -.
DR AlphaFoldDB; B2SQQ1; -.
DR SMR; B2SQQ1; -.
DR STRING; 360094.PXO_04530; -.
DR EnsemblBacteria; ACD57878; ACD57878; PXO_04530.
DR KEGG; xop:PXO_04530; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_3_6; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000001740; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 3.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW Transcription; Transferase.
FT CHAIN 1..1383
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000141750"
FT HELIX 6..10
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 30..40
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 54..61
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 67..70
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 72..80
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 87..93
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 98..109
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 119..133
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 137..139
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 141..143
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 146..150
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 152..156
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 158..169
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 176..182
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 184..186
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 189..192
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 195..197
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 200..205
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 211..218
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 222..229
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 251..253
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 278..286
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 295..300
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 310..312
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 314..317
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 325..334
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 346..348
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 352..359
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 365..376
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 384..395
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 398..401
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 405..415
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 429..433
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 438..447
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 452..465
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 476..478
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 479..482
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 484..506
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 517..519
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 523..535
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 538..542
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 548..555
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 556..560
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 562..565
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 568..570
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 573..576
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 580..582
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 583..585
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 586..589
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 593..595
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 597..600
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 611..613
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 615..622
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 624..635
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 636..641
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 652..655
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 660..673
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 685..687
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 691..693
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 694..697
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 699..701
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 704..713
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 714..716
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 727..729
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 733..740
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 742..745
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 750..755
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 757..764
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 766..768
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 779..781
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 785..787
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 811..814
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 820..823
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 826..834
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 837..840
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 841..847
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 850..853
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 859..871
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 888..890
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 892..894
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 898..900
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 911..913
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 926..934
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 956..964
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 972..1007
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1054..1077
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1087..1099
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1110..1112
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1116..1120
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1123..1125
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1136..1139
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1143..1147
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1151..1174
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1179..1190
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1203..1206
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1209..1217
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1228..1230
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1234..1243
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1248..1252
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 1257..1259
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1267..1274
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1281..1284
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1286..1290
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1295..1298
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1305..1307
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1310..1312
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1314..1322
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1326..1333
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 1334..1338
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1340..1352
FT /evidence="ECO:0007829|PDB:6J9E"
FT HELIX 1363..1373
FT /evidence="ECO:0007829|PDB:6J9E"
FT TURN 1374..1376
FT /evidence="ECO:0007829|PDB:6J9E"
FT STRAND 1377..1381
FT /evidence="ECO:0007829|PDB:6J9E"
SQ SEQUENCE 1383 AA; 154272 MW; B45F8B13DD1CCFDB CRC64;
MTSYSFTEKK RIRKDFGKQR SILEVPFLLA IQVDSYREFL QEDVESTKRK DLGLHAALKS
VFPISSYSGN AALEYVGYKL GQPVFDEREC RQRGMSYGAP LRVTVRLVIY DRESSTKAIK
YVKEQEVYLG EIPLMTGNGT FIVNGTERVI VSQLHRSPGV FFDHDRGKTH SSGKLLYSAR
IIPYRGSWLD FEFDPKDALF TRIDRRRKLP VSILLRALGY NNEEMLAEFF EINTFHINPD
EGVQLELVPE RLRGETLNFD LADGDKVIVE AGKRITARHV KQLEAAGVAA LAVPDDYLVG
RILSHDVVDG STGELLANAN DEISEDQLTA FRKAGVDAVG TLWVNDLDRG PYLSNTLRID
PTKTQLEALV EIYRMMRPGE PPTKEAAQNL FHNLFFTFER YDLSTVGRMK FNRRVGRKDV
LGESVLYDKK YFAERNDEES KRLVAEHTDT SDILEVIKVL TEIRNGRGVV DDIDHLGNRR
VRSVGEMAEN VFRVGLVRVE RAVKERLSMA ESEGLTPQEL INAKPVAAAI KEFFGSSQLS
QFMDQNNPLS EVTHKRRVSA LGPGGLTRER AGFEVRDVHP THYGRVCTIE TPEGPNIGLI
NSLAVFARTN QYGFLETPYR KVLDGKVSDD VEYLSAIEEN EYVIAQANAL TDAKNMLTEQ
FVPCRFQGES LLKPPSEVHF MDVSPMQTVS VAAALVPFLE HDDANRALMG ANMQRQAVPT
LRSQKPLVGT GIERAVARDS GVTVNALRGG VIEQIDAARI VVKVNEAEIG GGTDAGVDIY
NLIKYTRSNQ NTCINQRPLV NVGDVIARGD VLADGPSTDI GELALGQNML IAFMPWNGYN
FEDSILLSER VVEEDRYTTI HIEELTCVAR DTKLGPEEIS ADIPNVSEQA LNRLDESGVV
YIGAEVRAGD IMVGKVTPKG ESQLTPEEKL LRAIFGEKAS DVKDSSLRVP PGMDGTVIDV
QVFTRDGIEK DKRARQIEEN EIKRVKKDFD DQFRILEAAI YARLRSQIVG KVANGGANLK
KGDSVTDAYL DGLKKSDWFQ LRMKDEDAAD AIERAQKQIQ AHEKEFEARF ADKRGKITQG
DDLAPGVLKM VKVFLAVKRR IQPGDKMAGR HGNKGVVSNV VPVEDMPYMA TGESVDIVLN
PLGVPSRMNI GQILEVHLGW AAKGLGRKIQ RMLEAQAAVS ELRKFLDDIY NHDNAINAQR
VDLSQFSDEE LLNLGKNLID GVPMATPVFD GASEAEIKRM LELADLPQSG QTQLYDGRTG
EAFDRKTTVG YMHYLKLNHL VDDKMHARST GPYSLVTQQP LGGKAQFGGQ RFGEMEVWAL
EAYGAAYTLQ EMLTVKSDDV QGRNQMYKNI VDGEHEMVAG MPESFNVLVK EIRSLAIHME
LEE
