ID   RPOC1_CUSRE             Reviewed;         691 AA.
AC   A7M957;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 2.
DT   03-AUG-2022, entry version 50.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta';
DE            EC=2.7.7.6;
DE   AltName: Full=PEP;
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta';
DE            Short=RNA polymerase subunit beta';
GN   Name=rpoC1;
OS   Cuscuta reflexa (Southern Asian dodder).
OG   Plastid.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Convolvulaceae; Cuscuteae; Cuscuta;
OC   Cuscuta subgen. Monogynella.
OX   NCBI_TaxID=4129;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND RNA EDITING.
RX   PubMed=17714582; DOI=10.1186/1471-2229-7-45;
RA   Funk H.T., Berg S., Krupinska K., Maier U.-G., Krause K.;
RT   "Complete DNA sequences of the plastid genomes of two parasitic flowering
RT   plant species, Cuscuta reflexa and Cuscuta gronovii.";
RL   BMC Plant Biol. 7:45-45(2007).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid.
CC   -!- RNA EDITING: Modified_positions=21 {ECO:0000269|PubMed:17714582};
CC       Note=Editing at position 21 is more efficient in photosynthetically
CC       active tissue.;
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC1
CC       subfamily. {ECO:0000305}.
CC   -!- CAUTION: Young tissue from this organism is photosynthetic and contains
CC       some thylakoids, although the photosynthetic activity does not exceed
CC       the light compensation point. {ECO:0000305}.
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DR   EMBL; AM711640; CAM98385.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; YP_001430099.1; NC_009766.1.
DR   AlphaFoldDB; A7M957; -.
DR   SMR; A7M957; -.
DR   PRIDE; A7M957; -.
DR   GeneID; 5536664; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0009536; C:plastid; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.274.100; -; 1.
DR   Gene3D; 4.10.860.120; -; 1.
DR   HAMAP; MF_01323; RNApol_bact_RpoC1; 1.
DR   InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR000722; RNA_pol_asu.
DR   InterPro; IPR006592; RNA_pol_N.
DR   InterPro; IPR007080; RNA_pol_Rpb1_1.
DR   InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR   InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR   InterPro; IPR034678; RNApol_RpoC1.
DR   PANTHER; PTHR19376; PTHR19376; 1.
DR   Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR   Pfam; PF00623; RNA_pol_Rpb1_2; 2.
DR   SMART; SM00663; RPOLA_N; 1.
PE   2: Evidence at transcript level;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid; RNA editing;
KW   Transcription; Transferase.
FT   CHAIN           1..691
FT                   /note="DNA-directed RNA polymerase subunit beta'"
FT                   /id="PRO_0000308475"
SQ   SEQUENCE   691 AA;  79672 MW;  A95351C1E454055D CRC64;
     MNKNFSSMID RYKHQQLRIG LVSPQQISAW ATKILPNGEI VGEVKKPYTF LYKTNKPEKD
     GLFCERIFGP IKSGICACGN YRVIGDEKEE PKFCEQCGVE FVDSRIRRYR MGCIKLACPV
     THVWYLKRLP SYIANILDKP IKELEGLVYC DFYFARPITK KPTFLRLRGL LKYEIQSWKS
     SIPLFFTTQG FDTFRNREIS TGAGAIREQL ADLDLKISLE NSLLEWKYLG EEERIGNEWE
     DRKAGRRKGF LVRRMELVKH FIRTNIEPEW MVLSLLPVLP PELRPIIQID GGKLMSSDIN
     ELYRRVIYRN NTLTDLLTTS RSTPGELVMC QEKLVQEAVD TLLDNGIRGQ PMRDGHNKVY
     KSFSDIIEGK EGRFRETLLG KRVDYSGRSV IVVGPSLSLH RCGLPRKIAI ELFQPFVIRD
     LIRQHLASNI GVAKSKIREK EPIIWEILQE VMRGHPVLLN RAPTLHRLGI QAFQPVLVEG
     HVLCLHPLVC KGFNADFDGD QMAVHVPLSL EAQAEARLLM FSHMNLLSPA IGDPISVPTQ
     DMLIGLYVLT SDNRRDICTN RYTKCNIQTL QTKSSDSSNS KSKNWYKNRP FFCNSYDAIG
     AYRQKQINLE SPLWLRWRLD RRVITSSETP IEVHYESRGT FSEIYGHFLI VRSLKKKILF
     IYLRTTVGHI SLYREIEEAI QGFSRAWSSD T