ATR_ORYSI
ID ATR_ORYSI Reviewed; 2710 AA.
AC A2YH41;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 2.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=Serine/threonine-protein kinase ATR;
DE EC=2.7.11.1;
GN ORFNames=OsI_023634;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: Probable serine/threonine kinase. Seems to play a central
CC role in cell-cycle regulation by transmitting DNA damage signals to
CC downstream effectors of cell-cycle progression. May recognize the
CC substrate consensus sequence [ST]-Q and phosphorylate histone variant
CC H2AX to form H2AXS139ph at sites of DNA damage, thereby regulating DNA
CC damage response mechanism (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the PI3/PI4-kinase family. ATM subfamily.
CC {ECO:0000305}.
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DR EMBL; CM000131; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR SMR; A2YH41; -.
DR STRING; 39946.A2YH41; -.
DR PRIDE; A2YH41; -.
DR Proteomes; UP000007015; Chromosome 6.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR Gene3D; 1.10.1070.11; -; 1.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR003152; FATC_dom.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR036940; PI3/4_kinase_cat_sf.
DR InterPro; IPR018936; PI3/4_kinase_CS.
DR InterPro; IPR003151; PIK-rel_kinase_FAT.
DR InterPro; IPR014009; PIK_FAT.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR012993; UME.
DR Pfam; PF02259; FAT; 1.
DR Pfam; PF02260; FATC; 1.
DR Pfam; PF00454; PI3_PI4_kinase; 1.
DR Pfam; PF08064; UME; 1.
DR SMART; SM01343; FATC; 1.
DR SMART; SM00146; PI3Kc; 1.
DR SMART; SM00802; UME; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS51189; FAT; 1.
DR PROSITE; PS51190; FATC; 1.
DR PROSITE; PS00916; PI3_4_KINASE_2; 1.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell cycle; DNA damage; DNA repair; Kinase;
KW Nucleotide-binding; Nucleus; Reference proteome;
KW Serine/threonine-protein kinase; Transferase.
FT CHAIN 1..2710
FT /note="Serine/threonine-protein kinase ATR"
FT /id="PRO_0000295895"
FT DOMAIN 1647..2257
FT /note="FAT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00534"
FT DOMAIN 2368..2680
FT /note="PI3K/PI4K catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT DOMAIN 2678..2710
FT /note="FATC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00534,
FT ECO:0000255|PROSITE-ProRule:PRU00535"
FT REGION 2374..2380
FT /note="G-loop"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT REGION 2545..2553
FT /note="Catalytic loop"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT REGION 2565..2589
FT /note="Activation loop"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
SQ SEQUENCE 2710 AA; 303493 MW; BA4B9B8FDE6FF961 CRC64;
MANFSSHIQE LRELIAASST TTSTSAPASV HFEVKLREVL PNLLRDYVVP SSPTADGREA
TAVLKLLSYT AGKFPGVFFH GRAADVIRVI GRVLPFFAEP NFRSRHEIIF DTVWSLLSLL
RTGDREAYRQ FFLDVMVAVQ DVLYVVASMH GDRPSGVLTE RYLVKCLCGS FSDILDSPGI
FSDLPDSCQP KNGPGVLVDL TGETRWRPFA TMLIKLVNKC LADGTLYVEG LVNMPFVSAA
CSIICYGDES LHKVCFDFAR IVATVITVEI LPVENIIRSI MCILSQDVNG LSDIRDADYD
FSMGACLHAL HSSCPGYIVA ITASDIVNVF QRAVHTSRSS ELQVAMCNAY KRIVELCSPR
VWKPEILLKL LCLPKPCAKL IECIRLVVDK SGQSFLSSDD RDDGSSLLAK SEGLDLPKVG
QKRIALDEEN SFPKRLKMTE PRFSSGSFMV DELSAGVGQE LEKDHGCDFR VQLYSLINCL
SPDNHMAYPL EPAIAIQVLS LLCLSLSVYP KTNLFSRISK QVLSWIPWIC KQTTKICMFS
FDVSLYFEAV QTVMLLQSFL PGHTKLFEDE PLLIGNGCTD FEYPRYADLI NLLKLVSDDG
YLTSQTCSEK LKCLAVQIIA KIGSRQNAEC DLQVLELAIQ SETGELQNEA LMSLPIIVLY
SGPRMLGAMF RKLETIGTLG CKKLWKSIAI SLGFLSCLNG TTDCTDKVGN HCKLFLAKHC
EQPILTLNLL RGFWCPQCDV RTVHIEDQVP IVDIALSEDK NIDFKINMFK AHSLFFKFLY
AETSEECIVS IVEVLPRILK HSSRDVLLDM KFQWVQCVDF LLLHEMKAVR DAFSSVVSCF
LETNAMDILF SDGTGMSGGT SRVKFMDKIK SAFTEAEDPQ ILLTLLESTA AIVKASDIHG
EVFFCSFVLL IGQLGNHDYI VRVTALRLLQ RCCTYCFKGG LELFLSKYFH VRDNLYDYLS
SRLLTHPVVI SEFAESVLGV KTEELIRRMV PSIIPKLIVS HQNNDQAVVT LNELASHLNS
ELVPLIVNSL PKVLSFALFY EDGQHLSSVL QFYHTETGTD SKEIFSAALP TLLDEIICFP
GESDQIETDR RMAKISPTIQ NIARILIGND NLPEFLKNDF VRLLNSIDKK MLHSSDVNLQ
KQALQRIRKL VEMMGPYLST HAPKIMVLLI FAIDKETLQM DGLDVLHFFI KRLAEVSCTS
IKYVMSQVVA AFIPSLERCR ERPLVHLGKI VEILEELVVK NIILLKQHIR ELPLLPSLPS
LSGVNKVIQE ARGLMTLQDH LKDAVNGLNH ESLNVRYMVA CELNKLFNDR REDITSLIIG
EDIADLDIIS SLIMSLLKGC AEESRTVVGQ RLKLVCADCL GALGAVDPAK FKVMSCERFK
IECSDDDLIF ELIHKHLARA FRAASDTTVQ DSAALAIQEL LKLSGCQSLP NESSSCKMSK
RGQKLWGRFS SYVKEIIAPC LTSRFHLPSV NDATLAGPIY RPTMSFRRWI YYWIRKLTSH
ATGSRSGIFG ACRGIVRHDM PTAIYLLPYL VLNVVCYGTP EARQSITEEI LSVLNAAASE
SSGAIVHGIT GGQSEVCIQA VFTLLDNLGQ WVDDLKQEIA LSQSNYAMAG RQGGKLRDES
NSMYDQDQLL VQCSNVAELL AAIPKVTLAK ASFRCQAHAR ALMYFESHVR EKSGSSNPAA
DCSGAFSDDD ISFLMEIYGG LDEPDGLLGL ANLRKSSTLQ DQLIINEKAG NWAEVLTLCE
QSLQMEPDSV HRHCDVLNCL LNMCHLQAMI AHVDGLVYRI PQSKKTWCMQ GVQAAWRLGR
WDLMDEYLAE ADKGLVCRSS ENNASFDMGL AKIFNAMMKK DQFMVAEKIA QSKQALLVPL
AAAGMDSYMR AYPYIVKLHM LRELEDFNSL LGDESFLEKP FAADDPKFLK LTKDWENRLR
CTQPSLWARE PLLAFRRMVY NLSHMNAQAG NCWLQYARLC RLAGHYETAH RAILEADASG
APNAHMEKAK YLWNIRKSDS AIAELQQTLL NMPADVLGPT VLSSLSSLSL ALPNAPLSVT
QASKENPDVS KTLLLYTRWI HYTGQKQSND IKSLYSRVAD LRPKWEKGFF CIAKFYDDLL
VDARRRQEDK KIASGVGPVP PSSTGSLTTA TEEKPWWDML PVVLIQYARG LHRGHKNLFQ
ALPRLLTLWF EFGSIYIQDG SSFNKPMKEV HIRLLGIMRG CLKDLPPYQW LTVLSQLISR
ICHQNIEVVK LVKCIVTSIL REYPQQALWM MAAVSKSTVA ARRDAAAEIL QSAKKGSRRG
SDSNALFMQF PSLIDHLIKL CFHPGQPKAR AINISTEFSS LKRMMPLGII LPIQQALTVT
LPSYDTNMTD QSTFRPFSVS EHPTIAGIAD DAEILNSLQK PKKVVFIGSD GISRPFLCKP
KDDLRKDSRM MEFNAMINRL LSKVPESRRR KLYIRTFAVV PLTEDCGMVE WVPNTRGLRQ
ILQDIYITCG KFDRMKTNPQ IKKIYDQLQG KMPEEMLKAK ILPMFPPVFH KWFLTTFSEP
AAWIRARAAY AHTTAVWSMV GHIVGLGDRH GENILLDSTT GDCIHVDFSC LFDKGLLLEK
PEVVPFRFTQ NMVDGLGITG YEGVFVKVCE ITLSVLRTHK EALMTVLETF IHDPLVEWTK
SHKSSGVEVR NPHAQRAISN ITERLQGVVV GVNAAPSLPL SVEGQARRLI AEAVSHSNLG
KMYVWWMAWF
