RPOC1_OEDCA
ID RPOC1_OEDCA Reviewed; 1947 AA.
AC B2X1Z3;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-2008, sequence version 1.
DT 03-AUG-2022, entry version 44.
DE RecName: Full=DNA-directed RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01323};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01323};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01323};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01323};
DE Short=RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01323};
GN Name=rpoC1 {ECO:0000255|HAMAP-Rule:MF_01323};
OS Oedogonium cardiacum (Filamentous green alga).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC OCC clade; Oedogoniales; Oedogoniaceae; Oedogonium.
OX NCBI_TaxID=55995;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=SAG 575-1b / CCAP 575/1B / UTEX LB 40;
RX AGRICOLA=IND44059346; DOI=10.1111/j.1529-8817.2008.00510.x;
RA Turmel M., Brouard J.-S., Gagnon C., Otis C., Lemieux C.;
RT "Deep division in the Chlorophyceae (Chlorophyta) revealed by chloroplast
RT phylogenomic analyseS.";
RL J. Phycol. 44:739-750(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SAG 575-1b / CCAP 575/1B / UTEX LB 40;
RX PubMed=18558012; DOI=10.1186/1471-2164-9-290;
RA Brouard J.-S., Otis C., Lemieux C., Turmel M.;
RT "Chloroplast DNA sequence of the green alga Oedogonium cardiacum
RT (Chlorophyceae): unique genome architecture, derived characters shared with
RT the Chaetophorales and novel genes acquired through horizontal transfer.";
RL BMC Genomics 9:290-290(2008).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01323}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01323};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01323};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01323};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01323};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01323};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01323}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01323}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC1
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01323, ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; EF587366; ABU88206.1; -; Genomic_DNA.
DR EMBL; EU677193; ACC97249.1; -; Genomic_DNA.
DR RefSeq; YP_002000409.1; NC_011031.1.
DR AlphaFoldDB; B2X1Z3; -.
DR SMR; B2X1Z3; -.
DR PRIDE; B2X1Z3; -.
DR GeneID; 6440073; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.274.100; -; 1.
DR Gene3D; 4.10.860.120; -; 1.
DR HAMAP; MF_01323; RNApol_bact_RpoC1; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR034678; RNApol_RpoC1.
DR PANTHER; PTHR19376; PTHR19376; 2.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 2.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 2.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR SMART; SM00663; RPOLA_N; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Magnesium; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1947
FT /note="DNA-directed RNA polymerase subunit beta'"
FT /id="PRO_0000353503"
FT BINDING 119
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01323"
FT BINDING 121
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01323"
FT BINDING 141
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01323"
FT BINDING 144
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01323"
FT BINDING 1778
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01323"
FT BINDING 1780
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01323"
FT BINDING 1782
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01323"
SQ SEQUENCE 1947 AA; 232996 MW; D272333B2DCCE9DA CRC64;
MIKKNLFKDF SRFSFVQSSI KNNVRCHSKL KLVQPFFIKT KQERNKKFLS SYKFSEIQLI
TIDLASPEKI KEWAEKKLPN GKIFGQVTNP NTLHYKTLKP LKGGLFCERI FGPIKDFECA
CGIQKQKPFN RIISFQNKQF CSKCDIEYTW SDIRRYQLGY IQLVTPVTHL WYLKGMPSYI
RSVLGAKKKH IEAIAYCSEI VTIDQAWKPN RLIPFPETIS SVALSSKKRN TFVNKEIVNI
QLTYNNFKKF NVNNTRSLYV SINKKLFKKQ FSNNSYNCTC VDNEIQNPIF FLKYFNINFL
KPQNLKELKV LYINPDFYKV ENYPYKNQVK FEFYQKICRS FIKTSLNNHK PIYALNYLSH
SFLNSKVKIF SHFEHIDIHT MYVFNEEILI NRFSNLARLK KNGKSKKKIG YIHSKKIHIN
INKHLLYFKN KKLKNLQLSN NLKLIIIISN NASLNYFKNW SNTFNCMNTL ILKMQFVNKK
LHFQYYRSFF NTHTIERVYF NFFSLDYLKN SNFFVNFSKL LALNLLKFMK SNLFNIFIIP
IPTILDNFIF KLSLDSQFQR LLNSLSIQKQ KVLNSNSVIS KSNKIFKKQF FIKNFSLYDE
AIEILWQRFW KFGYYFTSNN MNNKLCKIIT KFNKNNNGLR STLPSWLKLI KKLIKKTQID
KKSNQNLLNF RVNVIHTNTQ NKFSLFVNLI KIYLKKVLFL FQIAPENIKN IYIKKSIFIF
QELRNKNFKY RNFRTQIKKI VIKLFGLELL PWVLSQFIFT SKIFYQFQKS KFSFYHNSNF
LTFSRRHSGR NLIKKIKESS FLNFQIGVNN INTIFHYKYS FNCLPSCHIP NYKCRQKFFI
YKNKIENILK LKKFRLFFNI WLSRFLKNNI SVLNVVVNLK LNNINSNNLQ TLNLLFYQKK
WLLKKKQIFY NYAMLKEFHS KLLIVGTLHD FSNFIKLIYS SSYKNETLIN IFSIQIQPFY
KFNFIQKIRK YLNSEYYNSF DSGRTKHYLR KFLNKDSDNL FFKQFNYVYY FILFSSIIST
QTIERGSLTS LNNQQFFLNL LCFFKLAKKN IFKKYSWLTS IQKYFYSRQI IKYKKKQQQK
SMLKFVKKKT FFNKFLDLIN SFIYDYQKRL KLLKKHSIIQ LILKKKMYIF YFLKKLQNKL
INEIQKYYFY EFEKKKFINI EFNLNNFKNI VIFMNLKKKI LFLNSVLFQS QTNFFNKHAN
LNIFNNSYNY MYIDFNNFLS IGQFWQILIN IFKNKINKYY LLSRSLPFII SNDYVKIDPL
IKKFHDDLKH QVCFNSFLIK LTFYNSTLLY PLQNIIKNNN KVLQTRQQYY KIIKSNNLFL
TNYFKKIRGN FTELSIKKFE NIHTIVSCIG YFGYQDILYH QIRELKKNDY FRNFYLKFIK
HDLSNNFNEA IFSVISESRI SYNDSIFWNN IYSLSNRYSW KSDIELNIFL CYMLSPETPS
DILIPLYKQR IHSSNVLREE PPIAGGGIIL KLLKELDLPE MKKIDIQLQT ILKKIPNKLH
EIEVLLKHNQ TNLKLLKYYS LKRRTVIEIE KSILRKLKYI RRLNWVSAKP EYMIIRNLPV
LPADLRPIFK IQQQLTASDL NRLYQKVIYR NDRLKRFLKN STTSNSFEML FAQRMLQEAV
DNLIENGKDI NSIETDSRGR PLKSLGELLK GKKGRFRQNL LGKRVDYSGR SVIVVGPKLK
LHECGLPIEM AIELFLPFLI KEILKNKYAL TVRSAKIYIK KHRIKITQLL RQVVKRQPIL
LNRAPTLHRL GIQAFLPKLV EGKAILLHPL VCSAFNADFD GDQMAVHVPI TNEARIEAWK
LVLSRNNLLS PATGEPILLP SQDMVLGCYY LTIENLQSLT QKKFRINRKI LNQRNYIFSD
FEKIFIAYQR NQINLHTNIW VIFNNTIESD SILQEPTEIQ VLFSGQYIKV YSDYYQKFNR
NGKIYNQVVR TTPGRILFNL MLNNCFN
