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ATR_YEAST
ID   ATR_YEAST               Reviewed;        2368 AA.
AC   P38111; D6VQD2; Q02580;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   03-AUG-2022, entry version 199.
DE   RecName: Full=Serine/threonine-protein kinase MEC1;
DE            EC=2.7.11.1;
DE   AltName: Full=ATR homolog;
DE   AltName: Full=DNA-damage checkpoint kinase MEC1;
DE   AltName: Full=Mitosis entry checkpoint protein 1;
GN   Name=MEC1; Synonyms=ESR1, SAD3; OrderedLocusNames=YBR136W;
GN   ORFNames=YBR1012;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INDUCTION.
RX   PubMed=8065923; DOI=10.1093/nar/22.15.3104;
RA   Kato R., Ogawa H.;
RT   "An essential gene, ESR1, is required for mitotic cell growth, DNA repair
RT   and meiotic recombination in Saccharomyces cerevisiae.";
RL   Nucleic Acids Res. 22:3104-3112(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7926756; DOI=10.1101/gad.8.6.652;
RA   Weinert T.A., Kiser G.L., Hartwell L.H.;
RT   "Mitotic checkpoint genes in budding yeast and the dependence of mitosis on
RT   DNA replication and repair.";
RL   Genes Dev. 8:652-665(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8091856; DOI=10.1002/yea.320100002;
RA   Becam A.-M., Cullin C., Grzybowska E., Lacroute F., Nasr F.,
RA   Ozier-Kalogeropoulos O., Palucha A., Slonimski P.P., Zagulski M.,
RA   Herbert C.J.;
RT   "The sequence of 29.7 kb from the right arm of chromosome II reveals 13
RT   complete open reading frames, of which ten correspond to new genes.";
RL   Yeast 10:S1-S11(1994).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA   Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA   Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA   Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA   Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA   Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA   Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA   Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA   Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA   Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA   Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA   Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA   Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA   Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA   Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA   Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA   Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA   Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA   Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA   Mewes H.-W., Kleine K.;
RT   "Complete DNA sequence of yeast chromosome II.";
RL   EMBO J. 13:5795-5809(1994).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [6]
RP   FUNCTION.
RX   PubMed=8553072; DOI=10.1126/science.271.5247.357;
RA   Sanchez Y., Desany B.A., Jones W.J., Liu Q., Wang B., Elledge S.J.;
RT   "Regulation of RAD53 by the ATM-like kinases MEC1 and TEL1 in yeast cell
RT   cycle checkpoint pathways.";
RL   Science 271:357-360(1996).
RN   [7]
RP   PHOSPHORYLATION OF RPA2.
RX   PubMed=8986766; DOI=10.1073/pnas.93.26.15075;
RA   Brush G.S., Morrow D.M., Hieter P., Kelly T.J.;
RT   "The ATM homologue MEC1 is required for phosphorylation of replication
RT   protein A in yeast.";
RL   Proc. Natl. Acad. Sci. U.S.A. 93:15075-15080(1996).
RN   [8]
RP   INTERACTION WITH LCD1.
RX   PubMed=11060031; DOI=10.1093/emboj/19.21.5801;
RA   Rouse J., Jackson S.P.;
RT   "LCD1: an essential gene involved in checkpoint control and regulation of
RT   the MEC1 signalling pathway in Saccharomyces cerevisiae.";
RL   EMBO J. 19:5801-5812(2000).
RN   [9]
RP   PHOSPHORYLATION OF LCD1, AND INTERACTION WITH LCD1.
RX   PubMed=10950868;
RA   Paciotti V., Clerici M., Lucchini G., Longhese M.P.;
RT   "The checkpoint protein Ddc2, functionally related to S. pombe Rad26,
RT   interacts with Mec1 and is regulated by Mec1-dependent phosphorylation in
RT   budding yeast.";
RL   Genes Dev. 14:2046-2059(2000).
RN   [10]
RP   FUNCTION.
RX   PubMed=11140636; DOI=10.1038/35050000;
RA   Downs J.A., Lowndes N.F., Jackson S.P.;
RT   "A role for Saccharomyces cerevisiae histone H2A in DNA repair.";
RL   Nature 408:1001-1004(2000).
RN   [11]
RP   IDENTIFICATION AS A KINASE, FUNCTION, AND MUTAGENESIS OF ASP-2224 AND
RP   ASN-2229.
RX   PubMed=11095737; DOI=10.1073/pnas.250475697;
RA   Mallory J.C., Petes T.D.;
RT   "Protein kinase activity of Tel1p and Mec1p, two Saccharomyces cerevisiae
RT   proteins related to the human ATM protein kinase.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:13749-13754(2000).
RN   [12]
RP   FUNCTION, INTERACTION WITH LCD1, PHOSPHORYLATION OF LCD1, AND MUTAGENESIS
RP   OF VAL-225; SER-552; LEU-781; PHE-1179; ASN-1700; ASP-2224 AND ASP-2243.
RX   PubMed=11359899; DOI=10.1128/mcb.21.12.3913-3925.2001;
RA   Paciotti V., Clerici M., Scotti M., Lucchini G., Longhese M.P.;
RT   "Characterization of mec1 kinase-deficient mutants and of new hypomorphic
RT   mec1 alleles impairing subsets of the DNA damage response pathway.";
RL   Mol. Cell. Biol. 21:3913-3925(2001).
RN   [13]
RP   FUNCTION.
RX   PubMed=12181334; DOI=10.1091/mbc.02-02-0012;
RA   Enomoto S., Glowczewski L., Berman J.;
RT   "MEC3, MEC1, and DDC2 are essential components of a telomere checkpoint
RT   pathway required for cell cycle arrest during senescence in Saccharomyces
RT   cerevisiae.";
RL   Mol. Biol. Cell 13:2626-2638(2002).
RN   [14]
RP   FUNCTION.
RX   PubMed=12792653; DOI=10.1038/sj.embor.embor871;
RA   Redon C., Pilch D.R., Rogakou E.P., Orr A.H., Lowndes N.F., Bonner W.M.;
RT   "Yeast histone 2A serine 129 is essential for the efficient repair of
RT   checkpoint-blind DNA damage.";
RL   EMBO Rep. 4:678-684(2003).
RN   [15]
RP   FUNCTION.
RX   PubMed=15369670; DOI=10.1016/j.cell.2004.08.015;
RA   Lisby M., Barlow J.H., Burgess R.C., Rothstein R.;
RT   "Choreography of the DNA damage response: spatiotemporal relationships
RT   among checkpoint and repair proteins.";
RL   Cell 118:699-713(2004).
RN   [16]
RP   FUNCTION.
RX   PubMed=15458641; DOI=10.1016/j.cub.2004.09.047;
RA   Shroff R., Arbel-Eden A., Pilch D.R., Ira G., Bonner W.M., Petrini J.H.J.,
RA   Haber J.E., Lichten M.;
RT   "Distribution and dynamics of chromatin modification induced by a defined
RT   DNA double-strand break.";
RL   Curr. Biol. 14:1703-1711(2004).
RN   [17]
RP   PHOSPHORYLATION OF RTT107.
RX   PubMed=14988729; DOI=10.1038/sj.emboj.7600129;
RA   Rouse J.;
RT   "Esc4p, a new target of Mec1p (ATR), promotes resumption of DNA synthesis
RT   after DNA damage.";
RL   EMBO J. 23:1188-1197(2004).
RN   [18]
RP   FUNCTION, AND PHOSPHORYLATION OF SLX4.
RX   PubMed=15975089; DOI=10.1042/bj20050768;
RA   Flott S., Rouse J.;
RT   "Slx4 becomes phosphorylated after DNA damage in a Mec1/Tel1-dependent
RT   manner and is required for repair of DNA alkylation damage.";
RL   Biochem. J. 391:325-333(2005).
RN   [19]
RP   FUNCTION OF THE MEC1-LCD1 COMPLEX, AND PHOSPHORYLATION OF LCD1 AND RAD53.
RX   PubMed=16365046; DOI=10.1074/jbc.m507508200;
RA   Ma J.-L., Lee S.-J., Duong J.K., Stern D.F.;
RT   "Activation of the checkpoint kinase Rad53 by the phosphatidyl inositol
RT   kinase-like kinase Mec1.";
RL   J. Biol. Chem. 281:3954-3963(2006).
RN   [20]
RP   FUNCTION, INTERACTION WITH LCD1; RFA1 AND RFA2, AND MUTAGENESIS OF
RP   2360-MET--ILE-2362 AND 2367-PHE-TRP-2368.
RX   PubMed=16148046; DOI=10.1091/mbc.e05-05-0405;
RA   Nakada D., Hirano Y., Tanaka Y., Sugimoto K.;
RT   "Role of the C-terminus of Mec1 checkpoint kinase in its localization to
RT   sites of DNA damage.";
RL   Mol. Biol. Cell 16:5227-5235(2005).
RN   [21]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
CC   -!- FUNCTION: Serine/threonine protein kinase which activates checkpoint
CC       signaling upon genotoxic stresses such as ionizing radiation (IR),
CC       ultraviolet light (UV), or DNA replication stalling, thereby acting as
CC       a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-
CC       Q. Recruited in complex with protein LCD1 by the single-strand-binding
CC       protein complex RPA to DNA lesions in order to initiate the DNA repair
CC       by homologous recombination, after the MRX-complex and TEL1 are
CC       displaced. Phosphorylates LCD1 and RPA2, a subunit of RPA, involved in
CC       DNA replication, repair and recombination. Phosphorylates RAD9, CHK1
CC       and RAD53, which leads to the activation of the CHK1 and RAD53 kinases
CC       involved in DNA damage repair cascade. Phosphorylates histone H2A to
CC       form H2AS128ph (gamma-H2A) at sites of DNA damage, also involved in the
CC       regulation of DNA damage response mechanism. Phosphorylates also SLX4
CC       and RTT107 which are proteins involved in genome stability. Required
CC       for cell growth and meiotic recombination.
CC       {ECO:0000269|PubMed:11095737, ECO:0000269|PubMed:11140636,
CC       ECO:0000269|PubMed:11359899, ECO:0000269|PubMed:12181334,
CC       ECO:0000269|PubMed:12792653, ECO:0000269|PubMed:15369670,
CC       ECO:0000269|PubMed:15458641, ECO:0000269|PubMed:15975089,
CC       ECO:0000269|PubMed:16148046, ECO:0000269|PubMed:16365046,
CC       ECO:0000269|PubMed:8065923, ECO:0000269|PubMed:8553072}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC         [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC         threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC         Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC         EC=2.7.11.1;
CC   -!- SUBUNIT: Interacts with LCD1, which is required for localization MEC1
CC       to the RPA complex. Interacts directly with the RPA subunits RFA1 and
CC       RFA2. {ECO:0000269|PubMed:10950868, ECO:0000269|PubMed:11060031,
CC       ECO:0000269|PubMed:11359899, ECO:0000269|PubMed:16148046}.
CC   -!- INTERACTION:
CC       P38111; P47027: DPB11; NbExp=3; IntAct=EBI-6668, EBI-25984;
CC       P38111; Q04377: LCD1; NbExp=10; IntAct=EBI-6668, EBI-35652;
CC   -!- SUBCELLULAR LOCATION: Nucleus. Note=Localizes to nuclear DNA repair
CC       foci in response to DNA double strand breaks. The recruitment to DNA
CC       lesion sites requires its interaction with LCD1 and the presence of the
CC       RPA complex on DNA.
CC   -!- DEVELOPMENTAL STAGE: Induced during meiosis.
CC   -!- SIMILARITY: Belongs to the PI3/PI4-kinase family. ATM subfamily.
CC       {ECO:0000305}.
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DR   EMBL; X75891; CAA53494.1; -; Genomic_DNA.
DR   EMBL; U31109; AAA74482.1; -; Genomic_DNA.
DR   EMBL; D11088; BAA01860.1; -; Genomic_DNA.
DR   EMBL; Z36005; CAA85094.1; -; Genomic_DNA.
DR   EMBL; BK006936; DAA07252.1; -; Genomic_DNA.
DR   PIR; S46005; S46005.
DR   RefSeq; NP_009694.3; NM_001178484.3.
DR   PDB; 5X6O; EM; 3.90 A; C=1-2368.
DR   PDB; 6Z2W; EM; 2.82 A; E/F=1-2368.
DR   PDB; 6Z2X; EM; 3.20 A; E/F=1-2368.
DR   PDB; 6Z3A; EM; 3.80 A; E/F=1-2368.
DR   PDBsum; 5X6O; -.
DR   PDBsum; 6Z2W; -.
DR   PDBsum; 6Z2X; -.
DR   PDBsum; 6Z3A; -.
DR   AlphaFoldDB; P38111; -.
DR   SMR; P38111; -.
DR   BioGRID; 32836; 375.
DR   ComplexPortal; CPX-3621; ATR-ATRIP DNA damage-sensing kinase complex.
DR   DIP; DIP-799N; -.
DR   IntAct; P38111; 23.
DR   MINT; P38111; -.
DR   STRING; 4932.YBR136W; -.
DR   iPTMnet; P38111; -.
DR   MaxQB; P38111; -.
DR   PaxDb; P38111; -.
DR   PRIDE; P38111; -.
DR   EnsemblFungi; YBR136W_mRNA; YBR136W; YBR136W.
DR   GeneID; 852433; -.
DR   KEGG; sce:YBR136W; -.
DR   SGD; S000000340; MEC1.
DR   VEuPathDB; FungiDB:YBR136W; -.
DR   eggNOG; KOG0890; Eukaryota.
DR   HOGENOM; CLU_000178_4_0_1; -.
DR   InParanoid; P38111; -.
DR   OMA; YTVYSQM; -.
DR   BioCyc; YEAST:G3O-29090-MON; -.
DR   PRO; PR:P38111; -.
DR   Proteomes; UP000002311; Chromosome II.
DR   RNAct; P38111; protein.
DR   GO; GO:0070310; C:ATR-ATRIP complex; IPI:ComplexPortal.
DR   GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR   GO; GO:0005634; C:nucleus; IDA:SGD.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004672; F:protein kinase activity; IDA:SGD.
DR   GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR   GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR   GO; GO:0006325; P:chromatin organization; IEA:UniProtKB-KW.
DR   GO; GO:0000077; P:DNA damage checkpoint signaling; IBA:GO_Central.
DR   GO; GO:0006975; P:DNA damage induced protein phosphorylation; IMP:SGD.
DR   GO; GO:0006310; P:DNA recombination; IMP:SGD.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IMP:SGD.
DR   GO; GO:0006139; P:nucleobase-containing compound metabolic process; IC:ComplexPortal.
DR   GO; GO:1901857; P:positive regulation of cellular respiration; IMP:SGD.
DR   GO; GO:2000105; P:positive regulation of DNA-templated DNA replication; IMP:SGD.
DR   GO; GO:0006468; P:protein phosphorylation; IBA:GO_Central.
DR   GO; GO:0007131; P:reciprocal meiotic recombination; IMP:SGD.
DR   GO; GO:2000779; P:regulation of double-strand break repair; IC:ComplexPortal.
DR   GO; GO:0000723; P:telomere maintenance; IDA:SGD.
DR   GO; GO:0000722; P:telomere maintenance via recombination; IGI:SGD.
DR   Gene3D; 1.10.1070.11; -; 1.
DR   Gene3D; 1.25.40.10; -; 1.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR003152; FATC_dom.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR   InterPro; IPR036940; PI3/4_kinase_cat_sf.
DR   InterPro; IPR018936; PI3/4_kinase_CS.
DR   InterPro; IPR003151; PIK-rel_kinase_FAT.
DR   InterPro; IPR014009; PIK_FAT.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR012993; UME.
DR   Pfam; PF02259; FAT; 1.
DR   Pfam; PF02260; FATC; 1.
DR   Pfam; PF00454; PI3_PI4_kinase; 1.
DR   Pfam; PF08064; UME; 1.
DR   SMART; SM01343; FATC; 1.
DR   SMART; SM00146; PI3Kc; 1.
DR   SMART; SM00802; UME; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
DR   PROSITE; PS51189; FAT; 1.
DR   PROSITE; PS51190; FATC; 1.
DR   PROSITE; PS00915; PI3_4_KINASE_1; 1.
DR   PROSITE; PS00916; PI3_4_KINASE_2; 1.
DR   PROSITE; PS50290; PI3_4_KINASE_3; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; Chromatin regulator; DNA damage; DNA repair;
KW   Kinase; Meiosis; Nucleotide-binding; Nucleus; Reference proteome;
KW   Serine/threonine-protein kinase; Transferase.
FT   CHAIN           1..2368
FT                   /note="Serine/threonine-protein kinase MEC1"
FT                   /id="PRO_0000088836"
FT   DOMAIN          1399..1944
FT                   /note="FAT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00534"
FT   DOMAIN          2049..2352
FT                   /note="PI3K/PI4K catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT   DOMAIN          2336..2368
FT                   /note="FATC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00534,
FT                   ECO:0000255|PROSITE-ProRule:PRU00535"
FT   REGION          2055..2061
FT                   /note="G-loop"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT   REGION          2140..2368
FT                   /note="Binding to the RPA complex"
FT   REGION          2221..2229
FT                   /note="Catalytic loop"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT   REGION          2241..2265
FT                   /note="Activation loop"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT   MUTAGEN         225
FT                   /note="V->G: In MEC1-101; impairs both the G1/S and intra-S
FT                   damage checkpoints but not the G2/M damage checkpoint; when
FT                   associated with P-552 and S-781."
FT                   /evidence="ECO:0000269|PubMed:11359899"
FT   MUTAGEN         552
FT                   /note="S->P: In MEC1-101; impairs both the G1/S and intra-S
FT                   damage checkpoints but not the G2/M damage checkpoint; when
FT                   associated with S-225 and S-781."
FT                   /evidence="ECO:0000269|PubMed:11359899"
FT   MUTAGEN         781
FT                   /note="L->S: In MEC1-101; impairs both the G1/S and intra-S
FT                   damage checkpoints but not the G2/M damage checkpoint; when
FT                   associated with S-225 and P-552."
FT                   /evidence="ECO:0000269|PubMed:11359899"
FT   MUTAGEN         1179
FT                   /note="F->S: In MEC1-100; impairs both the G1/S and intra-S
FT                   damage checkpoints but not the G2/M damage checkpoint; when
FT                   associated with S-1700."
FT                   /evidence="ECO:0000269|PubMed:11359899"
FT   MUTAGEN         1700
FT                   /note="N->S: In MEC1-100; impairs both the G1/S and intra-S
FT                   damage checkpoints but not the G2/M damage checkpoint; when
FT                   associated with S-1179."
FT                   /evidence="ECO:0000269|PubMed:11359899"
FT   MUTAGEN         2224
FT                   /note="D->A: Impairs kinase activity; when associated with
FT                   K-2229."
FT                   /evidence="ECO:0000269|PubMed:11095737,
FT                   ECO:0000269|PubMed:11359899"
FT   MUTAGEN         2229
FT                   /note="N->K: Impairs kinase activity; when associated with
FT                   A-2224."
FT                   /evidence="ECO:0000269|PubMed:11095737"
FT   MUTAGEN         2243
FT                   /note="D->E: Impairs kinase activity."
FT                   /evidence="ECO:0000269|PubMed:11359899"
FT   MUTAGEN         2360..2362
FT                   /note="MYI->AAA: In MEC1-85; disrupts interaction with RFA1
FT                   and severely impairs kinase activity."
FT                   /evidence="ECO:0000269|PubMed:16148046"
FT   MUTAGEN         2367..2368
FT                   /note="FW->AA: In MEC1-87; decreases the level of MEC1 and
FT                   impairs viability."
FT                   /evidence="ECO:0000269|PubMed:16148046"
FT   CONFLICT        197
FT                   /note="N -> D (in Ref. 1; BAA01860)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        716
FT                   /note="S -> P (in Ref. 2; AAA74482)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1255
FT                   /note="K -> Q (in Ref. 2; AAA74482)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1276
FT                   /note="L -> G (in Ref. 2; AAA74482)"
FT                   /evidence="ECO:0000305"
FT   HELIX           5..17
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          21..23
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           48..55
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            56..58
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          61..63
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           68..82
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            85..89
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            93..95
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           99..112
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           118..129
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            130..134
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            136..138
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           140..143
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            144..148
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           149..160
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           162..164
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          165..167
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           172..188
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            192..194
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          197..199
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           200..202
FT                   /evidence="ECO:0007829|PDB:6Z2X"
FT   HELIX           210..228
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           234..252
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          259..261
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           268..283
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           294..304
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          307..309
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           312..318
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          333..335
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           337..354
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           357..359
FT                   /evidence="ECO:0007829|PDB:6Z2X"
FT   STRAND          360..362
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          364..366
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          369..371
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            380..382
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           391..402
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           403..405
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           412..418
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          421..423
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          426..428
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           432..444
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           454..470
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            471..473
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          484..486
FT                   /evidence="ECO:0007829|PDB:6Z2X"
FT   TURN            491..494
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          503..505
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            512..515
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           517..525
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            526..528
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           532..534
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           537..553
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          562..564
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           570..577
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           582..588
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            589..591
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           592..595
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          599..602
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           607..615
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           621..623
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           624..635
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           640..654
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           659..675
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           681..684
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            685..687
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           688..691
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           694..696
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            699..702
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           703..713
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           719..723
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           725..732
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           741..748
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          751..754
FT                   /evidence="ECO:0007829|PDB:6Z2X"
FT   HELIX           756..764
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           768..777
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           784..792
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          801..803
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          807..809
FT                   /evidence="ECO:0007829|PDB:6Z2X"
FT   HELIX           810..817
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           828..844
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          845..850
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           856..859
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           866..868
FT                   /evidence="ECO:0007829|PDB:6Z2X"
FT   HELIX           869..877
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           881..891
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           900..915
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           920..924
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           926..938
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           943..955
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           960..962
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            963..965
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           967..975
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           982..997
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1000..1004
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1009..1015
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            1018..1020
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1023..1026
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1029..1036
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1044..1047
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            1048..1051
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1057..1075
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1091..1104
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            1105..1107
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1110..1123
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          1128..1130
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          1138..1140
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          1146..1148
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1149..1162
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1164..1169
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1174..1190
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          1194..1196
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            1199..1202
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1207..1213
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1218..1224
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1226..1228
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            1250..1252
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1254..1266
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            1271..1273
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1278..1282
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1289..1303
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            1311..1315
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1316..1327
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          1332..1334
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1338..1369
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          1373..1375
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          1378..1380
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1381..1391
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          1392..1395
FT                   /evidence="ECO:0007829|PDB:6Z2X"
FT   STRAND          1397..1399
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1400..1404
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            1405..1408
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1410..1422
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            1431..1439
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1440..1443
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1451..1455
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1463..1467
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1468..1472
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1476..1486
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1494..1505
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1509..1513
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            1516..1518
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          1520..1523
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1531..1544
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1548..1552
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1554..1558
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1567..1583
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1587..1603
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            1609..1611
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            1615..1617
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1618..1634
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1639..1652
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1660..1674
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1680..1699
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1703..1710
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1712..1715
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1721..1732
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1735..1746
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1747..1750
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            1756..1760
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1761..1772
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            1773..1775
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1779..1792
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1798..1815
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1824..1836
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            1837..1839
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            1845..1847
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1848..1864
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1873..1890
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1895..1900
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1901..1905
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          1913..1915
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1916..1928
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1931..1941
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1949..1962
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           1970..1983
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            1984..1986
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          2014..2016
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           2020..2023
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          2057..2060
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          2063..2068
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          2074..2082
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           2085..2101
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           2105..2108
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            2109..2111
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          2119..2121
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          2127..2131
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          2133..2137
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           2138..2146
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            2147..2150
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           2157..2164
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           2169..2180
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            2185..2188
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           2189..2191
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           2196..2220
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          2229..2233
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            2234..2236
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          2239..2241
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           2250..2252
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          2253..2256
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           2266..2272
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   TURN            2276..2279
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           2280..2294
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           2296..2303
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           2306..2309
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          2313..2316
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           2317..2328
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   STRAND          2332..2334
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           2340..2351
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           2354..2357
FT                   /evidence="ECO:0007829|PDB:6Z2W"
FT   HELIX           2362..2364
FT                   /evidence="ECO:0007829|PDB:6Z2X"
SQ   SEQUENCE   2368 AA;  273342 MW;  C06AEF9F0484A615 CRC64;
     MESHVKYLDE LILAIKDLNS GVDSKVQIKK VPTDPSSSQE YAKSLKILNT LIRNLKDQRR
     NNIMKNDTIF SKTVSALALL LEYNPFLLVM KDSNGNFEIQ RLIDDFLNIS VLNYDNYHRI
     WFMRRKLGSW CKACVEFYGK PAKFQLTAHF ENTMNLYEQA LTEVLLGKTE LLKFYDTLKG
     LYILLYWFTS EYSTFGNSIA FLDSSLGFTK FDFNFQRLIR IVLYVFDSCE LAALEYAEIQ
     LKYISLVVDY VCNRTISTAL DAPALVCCEQ LKFVLTTMHH FLDNKYGLLD NDPTMAKGIL
     RLYSLCISND FSKCFVDHFP IDQWADFSQS EHFPFTQLTN KALSIVYFDL KRRSLPVEAL
     KYDNKFNIWV YQSEPDSSLK NVTSPFDDRY KQLEKLRLLV LKKFNKTERG TLLKYRVNQL
     SPGFFQRAGN DFKLILNEAS VSIQTCFKTN NITRLTSWTV ILGRLACLES EKFSGTLPNS
     TKDMDNWYVC HLCDIEKTGN PFVRINPNRP EAAGKSEIFR ILHSNFLSHP NIDEFSESLL
     SGILFSLHRI FSHFQPPKLT DGNGQINKSF KLVQKCFMNS NRYLRLLSTR IIPLFNISDS
     HNSEDEHTAT LIKFLQSQKL PVVKENLVIA WTQLTLTTSN DVFDTLLLKL IDIFNSDDYS
     LRIMMTLQIK NMAKILKKTP YQLLSPILPV LLRQLGKNLV ERKVGFQNLI ELLGYSSKTI
     LDIFQRYIIP YAIIQYKSDV LSEIAKIMCD GDTSLINQMK VNLLKKNSRQ IFAVALVKHG
     LFSLDILETL FLNRAPTFDK GYITAYLPDY KTLAEITKLY KNSVTKDASD SENANMILCS
     LRFLITNFEK DKRHGSKYKN INNWTDDQEQ AFQKKLQDNI LGIFQVFSSD IHDVEGRTTY
     YEKLRVINGI SFLIIYAPKK SIISALAQIS ICLQTGLGLK EVRYEAFRCW HLLVRHLNDE
     ELSTVIDSLI AFILQKWSEF NGKLRNIVYS ILDTLIKEKS DLILKLKPYT TLALVGKPEL
     GILARDGQFA RMVNKIRSTT DLIPIFANNL KSSNKYVINQ NLDDIEVYLR RKQTERSIDF
     TPKKVGQTSD ITLVLGALLD TSHKFRNLDK DLCEKCAKCI SMIGVLDVTK HEFKRTTYSE
     NEVYDLNDSV QTIKFLIWVI NDILVPAFWQ SENPSKQLFV ALVIQESLKY CGLSSESWDM
     NHKELYPNEA KLWEKFNSVS KTTIYPLLSS LYLAQSWKEY VPLKYPSNNF KEGYKIWVKR
     FTLDLLKTGT TENHPLHVFS SLIREDDGSL SNFLLPYISL DIIIKAEKGT PYADILNGII
     IEFDSIFTCN LEGMNNLQVD SLRMCYESIF RVFEYCKKWA TEFKQNYSKL HGTFIIKDTK
     TTNMLLRIDE FLRTTPSDLL AQRSLETDSF ERSALYLEQC YRQNPHDKNQ NGQLLKNLQI
     TYEEIGDIDS LDGVLRTFAT GNLVSKIEEL QYSENWKLAQ DCFNVLGKFS DDPKTTTRML
     KSMYDHQLYS QIISNSSFHS SDGKISLSPD VKEWYSIGLE AANLEGNVQT LKNWVEQIES
     LRNIDDREVL LQYNIAKALI AISNEDPLRT QKYIHNSFRL IGTNFITSSK ETTLLKKQNL
     LMKLHSLYDL SFLSSAKDKF EYKSNTTILD YRMERIGADF VPNHYILSMR KSFDQLKMNE
     QADADLGKTF FTLAQLARNN ARLDIASESL MHCLERRLPQ AELEFAEILW KQGENDRALK
     IVQEIHEKYQ ENSSVNARDR AAVLLKFTEW LDLSNNSASE QIIKQYQDIF QIDSKWDKPY
     YSIGLYYSRL LERKKAEGYI TNGRFEYRAI SYFLLAFEKN TAKVRENLPK VITFWLDIAA
     ASISEAPGNR KEMLSKATED ICSHVEEALQ HCPTYIWYFV LTQLLSRLLH SHQSSAQIIM
     HILLSLAVEY PSHILWYITA LVNSNSSKRV LRGKHILEKY RQHSQNPHDL VSSALDLTKA
     LTRVCLQDVK SITSRSGKSL EKDFKFDMNV APSAMVVPVR KNLDIISPLE SNSMRGYQPF
     RPVVSIIRFG SSYKVFSSLK KPKQLNIIGS DGNIYGIMCK KEDVRQDNQY MQFATTMDFL
     LSKDIASRKR SLGINIYSVL SLREDCGILE MVPNVVTLRS ILSTKYESLK IKYSLKSLHD
     RWQHTAVDGK LEFYMEQVDK FPPILYQWFL ENFPDPINWF NARNTYARSY AVMAMVGHIL
     GLGDRHCENI LLDIQTGKVL HVDFDCLFEK GKRLPVPEIV PFRLTPNLLD ALGIIGTEGT
     FKKSSEVTLA LMRKNEVALM NVIETIMYDR NMDHSIQKAL KVLRNKIRGI DPQDGLVLSV
     AGQTETLIQE ATSEDNLSKM YIGWLPFW
 
 
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