RPOC1_TETOB
ID RPOC1_TETOB Reviewed; 1529 AA.
AC Q1KVT3;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=DNA-directed RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01323};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01323};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01323};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01323};
DE Short=RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01323};
GN Name=rpoC1 {ECO:0000255|HAMAP-Rule:MF_01323};
OS Tetradesmus obliquus (Green alga) (Acutodesmus obliquus).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Sphaeropleales; Scenedesmaceae; Tetradesmus.
OX NCBI_TaxID=3088;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UTEX 393;
RX PubMed=16638149; DOI=10.1186/1471-2148-6-37;
RA de Cambiaire J.-C., Otis C., Lemieux C., Turmel M.;
RT "The complete chloroplast genome sequence of the chlorophycean green alga
RT Scenedesmus obliquus reveals a compact gene organization and a biased
RT distribution of genes on the two DNA strands.";
RL BMC Evol. Biol. 6:37-37(2006).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01323}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01323};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01323};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01323};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01323};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01323};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01323}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01323}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC1
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01323, ECO:0000305}.
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DR EMBL; DQ396875; ABD48274.1; -; Genomic_DNA.
DR RefSeq; YP_635991.1; NC_008101.1.
DR AlphaFoldDB; Q1KVT3; -.
DR SMR; Q1KVT3; -.
DR PRIDE; Q1KVT3; -.
DR GeneID; 4099810; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.274.100; -; 1.
DR Gene3D; 4.10.860.120; -; 1.
DR HAMAP; MF_01323; RNApol_bact_RpoC1; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR034678; RNApol_RpoC1.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 2.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 2.
DR SMART; SM00663; RPOLA_N; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Magnesium; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1529
FT /note="DNA-directed RNA polymerase subunit beta'"
FT /id="PRO_0000353515"
FT BINDING 156
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01323"
FT BINDING 158
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01323"
FT BINDING 183
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01323"
FT BINDING 186
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01323"
FT BINDING 1328
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01323"
FT BINDING 1330
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01323"
FT BINDING 1332
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01323"
SQ SEQUENCE 1529 AA; 182051 MW; 7ABC52F2407063F5 CRC64;
MKNFKNQNFQ KENLNLSFSQ KRKKFSGKIG ICFFEYNFFQ KNFHVQNFPI KKCRLLAQVS
EQTPQISSFR QTSNFTPNLI KKKLLNGYSK IHELKLVTIE LASPEKIKAW AEKELPNGKI
FGEVTNANTF HYRTFKPSKG GLFCEHIFGP LKDFECACGK RQRPSALESR KILEHQKTSR
YFCPNCDVEY TWSIIRRYQL GYIKLNAPVT HLWYFKTNPS YLSILFDMKR RHLESIIYCT
ETITIENTWK YSQQTSILNR SPKDLYLTWQ KFFTLEEQLQ QYNRIYQNKH QHQKQRKLEF
RNLSILQNKI SPQINWKNFD QQIVEKNQNN SVFAKNFQTF ENIETFGLKR KHSIFENISM
QKQKKFGTYF FEEIWKIILQ KSYKNSFLFL NSQECFSEHF FQNIYRISNI FLFSSKKIQK
FLKFHEIFIF GNTESLNKTI LELKNKKRTV NFFSKDFTPF FIDQNVFESH NSLKIKKQYW
KSFFFLFEFT RFFLSKKNTK IQNLNVLSKK DFLFLVNLIP FLKKLALFQN LQVFKKNTKK
SHAFYIQNLS KKNKSLKKKI FLKKNLFTEQ NLISFQNQVQ KNKIFSVEPN FSEKIFSTFF
SNDFLNSNQT FEFNLFNLFF DVSLNFGKIL AFLKLYYEID FCSFVQKHSF DFEKNYSFKN
SFSTKQFELF SNQSTLVSMY ETVFFDYFSF FFHFLKIFKF SNFNMSNSVS LEFLNDSEKE
NIFYSQNFRT QKNFFFSLKA LSFLLVGFNY ENKQNLLSDP FDSGFEIFPN QNSVFMTQNF
FSSKLFFKEK KQKKNNSKKL KKQLEIFISN KPLLSRQNFT ERKKQIQNKK VQKFFDFDSM
TENFECSFSS EKKFKLFQKK VSNSSLQLTQ IQKKFKEPFL YKFIKQKNQK KKKFNNFHVF
LSTKANSNLF FFEKKQKQQI QEKFQKMEKN HFLQNSILTI AYNYLWNNDA DWKYFIYYNS
LFFYEFEDHP IFLYRSLSPI KDTKNNQAFM FSDTNSSAIK FGQSFSSFLS WSIDDLPKNF
FVGAGILEKL LTEYTSSELR KMTKQHQILL PKINQMLRFL KQNAKTKKDS LKIQKYFQKR
EQIIRRLKFL RKFSRRNSNP TFMILKNLPV LPPDLRPILK LQNQIAASDL NRFYQRIIYR
NDRLKKFAKD SATNQSFEIK YAQRLLQEAV DNLIQNGKGS VKAETNSRGQ PLKSLSEILK
GKQGRFRQYL LGKRVDYSGR SVIVVGPELK LYECGLPKEM ALELFLPFLI QYILQNKLAQ
TVVGAKNLLK SDSNLTLHLL HKVIKNIPIL LNRAPTLHRL GFQAFLPKLI EGRAILLHPM
VCPSFNADFD GDQMAVHIPL TVEARTEAWK FMLATNNLMN SATGEAIILP SQDMVLGCYY
LTLDFQSKFV GVQLSNLLKK QNSFFPFSKP TYLGKEKTFQ IQGKNFEKFG IQNKAFLLFS
NFLSVLNAYQ RKEISLHTPV WVKWNSNVDF GNEFSKPVEI RLQINGSWEE IQPKYTTFYN
YKNKQLQKII RTTPGRILMN FMIQQCSMS
