RPOC1_TUPAK
ID RPOC1_TUPAK Reviewed; 1578 AA.
AC Q3ZJ92;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=DNA-directed RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01323};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01323};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01323};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01323};
DE Short=RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01323};
GN Name=rpoC1 {ECO:0000255|HAMAP-Rule:MF_01323};
OS Tupiella akineta (Green alga) (Pseudendoclonium akinetum).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Chlorophyta; Ulvophyceae; OUU clade;
OC Ulotrichales; Tupiellaceae; Tupiella.
OX NCBI_TaxID=160070;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UTEX 1912;
RX PubMed=15930151; DOI=10.1093/molbev/msi182;
RA Pombert J.-F., Otis C., Lemieux C., Turmel M.;
RT "The chloroplast genome sequence of the green alga Pseudendoclonium
RT akinetum (Ulvophyceae) reveals unusual structural features and new insights
RT into the branching order of chlorophyte lineages.";
RL Mol. Biol. Evol. 22:1903-1918(2005).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01323}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01323};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01323};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01323};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01323};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01323};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01323}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01323}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC1
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01323}.
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DR EMBL; AY835431; AAV80599.1; -; Genomic_DNA.
DR RefSeq; YP_636175.1; NC_008114.1.
DR AlphaFoldDB; Q3ZJ92; -.
DR SMR; Q3ZJ92; -.
DR GeneID; 4108779; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.274.100; -; 1.
DR Gene3D; 4.10.860.120; -; 1.
DR HAMAP; MF_01323; RNApol_bact_RpoC1; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR034678; RNApol_RpoC1.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 2.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 2.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR SMART; SM00663; RPOLA_N; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Magnesium; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1578
FT /note="DNA-directed RNA polymerase subunit beta'"
FT /id="PRO_0000353513"
FT BINDING 101
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01323"
FT BINDING 103
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01323"
FT BINDING 115
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01323"
FT BINDING 118
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01323"
FT BINDING 1286
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01323"
FT BINDING 1288
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01323"
FT BINDING 1290
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01323"
SQ SEQUENCE 1578 AA; 183291 MW; 8F6EBD8C1A92488B CRC64;
MSSDLKYSLS SEPPQPQVQS PLLKILKGEV SETTELKKLH SFRLCLSSPK IIKQWAQRTL
PNGEIVGSIT NAQTVNYKTL KPEKGGLFCE RVFGPVKDFY CSCGKQKTKQ HPKVCPTCGV
QYISSQSRRY KMGYIELVSP VTHIWYLKSS PSYISLLLNL KKKNLEALTY CSENLSLNIK
SFQNELLFQN VLHLLKANPF ALEKGLKKRK KRFLVNSNWF SFIFDNSYLI KANSGFQLKV
KDFPFFLITK NQNLQNFMGV CSQSANGNEQ ILPEMEKQGK NTFQFSPFLT TVQKHGSSEN
GSFPELKREK DELELFKLID SFSSKSPSKK KDFFRSEILT AFYKKYSKFH FSRFRSNKKS
FLISLFSSSN NWYFNKNQNI GYFSIFNKNF KIFFSLLNYQ ILLKTIDKNS LANLLSPLFD
NYLNDDSQVL FDDSIKKLNL TKNFRSNEDQ ELSTLLNSCF FLNKIGAIAQ SPFSLAFYTQ
KSEKFYCFSP SFLNPFEKDS LSGKIFSLKK KKILPSQKMF ELKPLTLFCS KKTKRNEVES
ENPNSNHMLP FIAFSKKQRC EEKADFFKQL RMIELESEKS RSPKDLLFLN KFPFKNEKKL
KYNLQKRNKL HTLKNPIGNF KFRKYFSLRL DSSLKRSLLI YQIKNFKNGF EHSDFYSENQ
TSTNFTKFLV SRELEKSSKF SNSLFSFENF AQLLIFIFDF PAALERQRQS FSLRSYSKSA
NFRSQTSARG EVGGNFNLEV KQSQNYFLDW KLTNNYRNFY QSSEIQNLNC FFISNFLVKN
AIPVSPLSLE QFVFNNETQK NLHLGNEDSR FFANLKKPIK KLGLLKNSFL AETLANAKAN
AHNLKENRTP VIQDKENESQ TFSQLFFDQI DQKEGSSLKS KDLNFTNWNN KKITRFIELL
EKTLFLKKPG LVNNYYTISQ SLQWPCQKDW ARFLNYMTNT ADKTDSLIPS YLERGISFDL
VLTGAGSIKK LLSLFTPMGK KASIEIVAKQ INGTLLKLNR DIKRLEDFFK FEIFFIDDQE
IIEKVFMKLV ILRSLRSKAL RRLKVLRPFK GSHVLPEWMV LSVLPILPPA LRPIIPLDSQ
QVAVSDLNKL YQTVLFRNKR VQRFYNDYYS LNFSEEMRYA QRLLQEAVDA LIENGKGDSA
AITASNNRPL KSLSDMIKGK KGRFRQNLLG KRVDYSGRSV IVVGPKLRLH ECGLPKEMAI
ELFQPFLIRR LIFKEVATNF ISAKKLIKSN PESILDILRE VMENRPVLLN RAPTLHRLGI
QAFQPKLISG RAILLHPLVC AAFNADFDGD QMAVHIPLSF QACAEAWKLM GSRNNLLSPA
TGEPIILPSQ DMVLGCYYLT TLDRVKIKQK LSQSSFLFPF LISKQGVHEN LSTISSNSKG
IENWILKKPS QKSEIRTLSS FYEINESERA TFESKTNDTS ILKLQDKKRR ETLTFKRNRL
SPVDTKLHLM DLSKNKNQNL FLNRFEKRAN CKVWSDSNKY YSNWDQVLQS LNQQLIDLHS
PIWLRWNFYF EFVLKKESFL EIRLDKYGNS VYINPNYQSY SNSKLEKIVF YIRTTPGRVL
MNKLIFEALN KPSLKKSF
