RPOC2_AGRST
ID RPOC2_AGRST Reviewed; 1466 AA.
AC A1EA00;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 50.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Agrostis stolonifera (Creeping bentgrass).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Poodae; Poeae; Poeae Chloroplast Group 1 (Aveneae type);
OC Agrostidinae; Agrostis.
OX NCBI_TaxID=63632;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Penn A-4;
RX PubMed=17534593; DOI=10.1007/s00122-007-0567-4;
RA Saski C., Lee S.-B., Fjellheim S., Guda C., Jansen R.K., Luo H.,
RA Tomkins J., Rognli O.A., Daniell H., Clarke J.L.;
RT "Complete chloroplast genome sequences of Hordeum vulgare, Sorghum bicolor
RT and Agrostis stolonifera, and comparative analyses with other grass
RT genomes.";
RL Theor. Appl. Genet. 115:571-590(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; EF115543; ABK79572.1; -; Genomic_DNA.
DR RefSeq; YP_874728.1; NC_008591.1.
DR AlphaFoldDB; A1EA00; -.
DR GeneID; 4524922; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1466
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000277184"
FT REGION 618..725
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 618..633
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 651..683
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 705..722
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 220
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 296
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 303
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 306
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1466 AA; 168036 MW; 6F7DFF36DF387D0A CRC64;
MAERANLVFH NKEIDGTGMK RLISRLIDHF GMGYTSHILD QLKTLGFHQA TTTSISLGIE
DLLTIPSKGW LVQDAEQQSF LLEKHYYYGA IHAVEKLRQS VEIWYATSEY LKQEMNSNFR
ITDPSNPVYL MSFSGARGNA SQVHQLVGMR GLMADPQGQM IDLPIQSNLR EGLSLTEYII
SCYGARKGVV DTAVRTADAG YLTRRLVEVV QHILVRRRDC GTIQGISVSP QNGMTEKLFA
QTLIGRVLAD DIYIGSRCIA ARNQDIGIGL VNRFITAFRA QPFRAQPIYI RTPFTCRSTS
WICQLCYGRS PTHSDLVELG EAVGIIAGQS IGEPGTQLTL RTFHTGGVFT GGTADLVRSP
SNGKIQFTEN LVHPTRTRHG QPAFLCYIDL HVTIQSQDIL YSVSIPSKSL ILVQNDQYVK
SEQVIAEIRS GTSTLHFKER VQKHIYSESD GEMHWSTDVY HAPEYQYGNL RRLPKTSHLW
ILSGNICRSS IASFSLHKDQ DQMNTYGKKD REIFDYSRSD RIMANGHWNL IYPSIFQDNS
DLLAKKRRNR FVIPLQYHQE QEKELISCLG ISIEIPFMGV LRRNTIFAYF DDPRYRKDKK
GSGIVKFRYR TLEEEYRTRE EDLEDEYETL EDEYRTREDE YEYETLEDEY GSPENEYRTL
EKDSEDEYGS PESKYRTREG EYGTLEEDSE DGYGNPGESS EDKYGTLEED LEEDSEDEYD
SSEEDSILKK EGLIEHRGTK EFSLKYQKEV DRFFFILQEL HILPRSSSLK VLDNSIIGVN
TQLTKNTRSR LGGLVRVKRK KSHTELKIFS GDIHFPEEAD KVLGGCLIPP ERKKKDSKES
KKRKNWVYVQ RKKILKSKEK YFVFVRPAVA YEMDEGRNLA TLFPKDLLQE EENLQLRIVN
FISHENSKLT QRIYHTNSQF VRTCLVVNWE QEEKEKAGAS LVEVRANNLI RDFLRIELVK
STISYTRKRY DRTSAGPIPH NRLDHTNTNS FYSKAKIESL SQHQEATGTL LNRNKEYQPL
MILSASNCSR IGLFKNSKHP NAIKELNPRI PIQEIFGPLG VIVPSTSNFS SSYYLLTHNQ
SLLKKYLFLA NLKQTFQVLQ GLKYSLIDEN KRVSNFDSNI MLDPLLLNCH FVHHDSWEET
LAIIHLGQFI CENVCLFKSH IKKSGQIFIV NMDSFVIRAA KPYLATTGAT VNGHYGEILY
KGDRLVTFIY EKSRSSDITQ GLPKVEQIFE ARSIDSLSPN LERRIEDWNE RIPRILGVPW
GFLIGAELTI AQSRISLVNK IQKVYRSQGV QIHNRHIEII IRQVTSKVRV SEDGMSNVFS
PGELIGLLRA ERAGRALDES IYYRAILLGI TRASLNTQSF ISEASFQETA RVLAKAALRG
RIDWLKGLKE NVVLGGIIPV GTGFLKFVHR SPQDKNLYFE IQKQNLFASE MRDFLFLHTE
LVSSDSDVAN NFYETSEPPF TPIYTI
