RPOC2_AMBTC
ID RPOC2_AMBTC Reviewed; 1373 AA.
AC P60289;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 16-JAN-2004, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Amborella trichopoda.
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Amborellales; Amborellaceae; Amborella.
OX NCBI_TaxID=13333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12832641; DOI=10.1093/molbev/msg159;
RA Goremykin V.V., Hirsch-Ernst K.I., Wolfl S., Hellwig F.H.;
RT "Analysis of the Amborella trichopoda chloroplast genome sequence suggests
RT that Amborella is not a basal angiosperm.";
RL Mol. Biol. Evol. 20:1499-1505(2003).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; AJ506156; CAD45097.2; -; Genomic_DNA.
DR RefSeq; NP_904089.2; NC_005086.1.
DR AlphaFoldDB; P60289; -.
DR PRIDE; P60289; -.
DR GeneID; 2546586; -.
DR KEGG; atr:2546586; -.
DR OrthoDB; 731145at2759; -.
DR Proteomes; UP000017836; Chloroplast.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Reference proteome; Transcription;
KW Transferase; Zinc.
FT CHAIN 1..1373
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000067912"
FT BINDING 224
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 296
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 303
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 306
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1373 AA; 156104 MW; 281B7DFFE2F17569 CRC64;
MEVLMAERAD LVFHNKAIDG TAMKRLISRL IDHFGMAYTS HILDQVKTLG FRQATLTSIS
LGIDDLLTTP SKGWLVQDAE QQSLILEKHH HYGNVHAVEK LRQSIEIWYA TSEYLRQEMN
PNFRMTDPFN PVYIMSFSGA RGNASQVHQL VGMRGLMSDP QGQMIDLPIQ SNLREGLSLT
EYTISCYGAR KGVVDTAVRT SDAGYLTRRL VEVVQHIVVR RTDCGTIRGI SVSPRNGVGV
TERIFIQTLI GRVLANDVYM GLRCIATRNQ DIGIGLVNRF ITSRAQPIYI RSPFTCRSTS
WICQLCYGRS TTHGNLVELG EAVGIIAGQS IGEPGTQLTL RTFHTGGVFT GGIAEHVRAP
SNGKIKFNED LVHPTRTRHG HPAFLCHIDL HVTIESQDII HKVNIPPKSF LLVQNDQYVE
SEQVIAEIRA GTSTLKERVQ KHIYSDSEGE MHWSTDVYHT PEYTHGNVHL LPKTSHLWVL
SGSPCRSSIV PFPLHKDQDQ MNVQSLYVEE RYISDLSMNN DRVRHKLFGW DQKRGRVSYY
SGPDRIISNR NWDSIYPLIL HENSDLLAKR RRNRFIIPFQ YDQEQEKELR PPGIVIKIPI
KGILRRNSIL AYFDDPRYRR SSSGIAKYGT IEVDSIIKKE DLIEYRKTRE FGPKYQIQIK
VNRFFFIPEE VHILPRSSSI MVRNNSIIGV DTRITLNIRS QVGGLVRVEK KKKRIELKIS
SGDIHFPGET DNIARYSGIL IPPGRVKNTE SKFKNWIYVQ RITPIKKKYF VSVRPVVTYE
IADGINLATF FPQDLLQEKN NLQLRVVNYI LYGDGKPIRG IFHTSIQLVR TCLVLNWDQD
RAGSIEEEEA YTSLAEVRVN DLIRNFIRID LVKSPISSTG KENDMAGSGL IPNNGSDRIN
TNPFFSKAKT QSLSQHQGTI RTLLNRNKEG QGESLMVLSS SNCSRIGPLN GSKYHNVTKE
SIQEDPMISI RNSLGPLGTV PNILNFSSSY HSITHNEILF NKYLLPDNSR ETFLVPKSYF
MDENRRIYNL DPCSNIILTP FNLNWCFLHH DYWEDTSTII SLGQFLCENI CISKDGPCVK
SGQIIIVHVD SLVIRLAKYH LATRGATVHG HYGEILYEGD TLVTFIYEKS RSGDITQGLP
KVEQVLEVRS IDSISMNLEK RVEGWNERIT GFLGIPWEFF ISAQLTIVQS RISLVNKIQK
VYRSQGVQIH NRHIETIVRQ ITSKVLVSED GMSNVFSPRE LIGLLRAERI GRALEDDICY
RAILLGITRA SLNTQSFISE ASFQETTRVL AKAALRSRID WLKGLKENVV LGGMIPVGTG
FKGFVHHSRE HNNISLEIKK KNLFDGKMRD ILFYHREFCG SCIPKNFHDT SEQ
