RPOC2_ANEMR
ID RPOC2_ANEMR Reviewed; 1376 AA.
AC B0YPM0;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 47.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Aneura mirabilis (Parasitic liverwort) (Cryptothallus mirabilis).
OG Plastid; Non-photosynthetic plastid.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Marchantiophyta;
OC Jungermanniopsida; Metzgeriidae; Metzgeriales; Aneuraceae; Aneura.
OX NCBI_TaxID=280810;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=18056074; DOI=10.1093/molbev/msm267;
RA Wickett N.J., Zhang Y., Hansen S.K., Roper J.M., Kuehl J.V., Plock S.A.,
RA Wolf P.G., dePamphilis C.W., Boore J.L., Goffinet B.;
RT "Functional gene losses occur with minimal size reduction in the plastid
RT genome of the parasitic liverwort Aneura mirabilis.";
RL Mol. Biol. Evol. 25:393-401(2008).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; EU043314; ABS54467.1; -; Genomic_DNA.
DR RefSeq; YP_001687206.1; NC_010359.1.
DR AlphaFoldDB; B0YPM0; -.
DR GeneID; 5952191; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0009536; C:plastid; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Metal-binding; Nucleotidyltransferase;
KW Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1376
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000353544"
FT BINDING 220
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 289
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 296
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 299
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1376 AA; 158058 MW; 9604177FA2E33FC7 CRC64;
MAEPVDLIFY NRVMDRITIK QIMNGLMTHF GITYATHVLD QPKTLGFQQA TRGATSLGID
DLLTTPSKGW LIKDAEHHGN LSGKHYNYGN LYAVEKLRQL IETWYTTSEY LKRGMNLNFG
ITDPSNPVHM MSFSGARGST SQIHQLVGMR GLMSDPQGQI IDLPIQSNFR EGLSLTEYII
SCYGARKGVV DTAVRTSDAG YLTRRLVEVV QRIVVRKSDC GTTSGISMNH SWKKKRIIYQ
RLIGRVLAEN VYINNRCLAM RNQDIGNLTV DKFIGLTAEP IRVRSPSTCK SMLWICQLCY
GWSLTNGDLV EVGEAVGIIA GQSIGEPGTQ LTLRTFHTGG VFTGDIAEHV RTPSDGVIEF
DGRFVYPTRT RHGHPAWICH TSLFLSIRGR NKRHNLLIPA KSLLLIRNNQ YVESEQVIAE
IRTKASPPKE RIHKYIYSDL DGEMSWITGV CHTSEYMHSC IHPTLRTCHV WILSGKSDNK
KDKLPVSFYD NQDRINSNTF LAEDQSFPST PRNKNRSNIC IFNPYVSKKN RVFTRWELTH
IAFNSSNQSV NSNIILYDYD VVRKHRTFFL REMYTNLYFL LEVKNNGVLR NNDFFAITDH
PKYELKESGV LKYGTIKIGS ADRNNESRDE RMKKFRSTFR IVKGGNFFLI PERRYISAQV
LSSILERNNK SVRVGTSITS IVQSDTSGLV RIGRRINNTY EVKILPGIIY YPDEPNKLSE
RVSVLIPPGK QLFNKFRCDH WIYFQWIIPL EEKPFALPRP ATEYVISNRS TQKKFLNLLR
NIDLGINTAN YLLHADGEQI RVISGRCTPL IRTCLIICWG KKYFTEKAHV SLLKIKTTNN
SRNYLQISLI YFDSLDEKRQ GDMVYSQYIL RRNYYDDFFS ICRNRLKSKH KGIIRISSDR
KDEYRSSIIL SPPDLIEVSK MIGSKNPIGK DTDNFSSTEF FNFQNQLEKI GSIRNRIRSR
AKHFLRIVSF DEICFLGNLR TSTKLDECFY WIIYTGAKTC LRIDQSRQTF QNPKWFLIDE
YIGIYRCRCE KNINHNLFDY YLPSPSREGT KIPSLGHFLF KDFRMVETKQ FLPPGQVIGI
HMDYLIIRLG KPHLANEGAI IHNNCGGSVE EGDTLITLIY ERLKSGDIIQ GLPKVEQLLE
ARSTNSVSID LGSSFRDWSN DMKRFIGNLW GFFLSARISM EQGQMILVDQ IQKVYKSQGV
QISNKHIEII VRQMTSRFLT SEDGIIDISS PGELIEFSQA RRMNRVLEEV ISYEPILLGI
TEASLSTRSF ISEASFQETT RVLARAALKG RIDWLRGLKE NVIVGEIIPA GTGSEEVIWQ
RTLEREEDIF LSNREFCFGE NIENPFWHSD TFFVFPTMET VRNILRESAS ANGRNI
