RPOC2_ANGEV
ID RPOC2_ANGEV Reviewed; 1414 AA.
AC A2T322;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 49.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Angiopteris evecta (Mule's foot fern) (Polypodium evectum).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Polypodiopsida; Marattiidae; Marattiales; Marattiaceae; Angiopteris.
OX NCBI_TaxID=13825;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Roper J.M., Hansen S.K., Wolf P.G., Karol K.G., Mandoli D.F.,
RA Everett K.D.E., Kuehl J.V., Boore J.L.;
RT "The complete plastid genome sequence of Angiopteris evecta (G. Forst.)
RT Hoffm. (Marattiaceae).";
RL Am. Fern J. 97:95-106(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; DQ821119; ABG79589.1; -; Genomic_DNA.
DR RefSeq; YP_001023690.1; NC_008829.1.
DR AlphaFoldDB; A2T322; -.
DR SMR; A2T322; -.
DR PRIDE; A2T322; -.
DR GeneID; 4788131; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1414
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000353545"
FT BINDING 220
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 293
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 300
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 303
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1414 AA; 162167 MW; BE28217C3F415A14 CRC64;
MADQAKLLFY NKMMDRTAIK HLIRRLISHF GITFTANILD QIKTLGFHQA TNASISLGID
DLLTAPLKSW LIQDAEKQGY SSEQHHRYGS VHSVEKLRQL IETWYATSEY LKQEMNPNFR
MTDPLNPVHM MSFSGARGSI SQVHQLVGMR GLMSDPQGQI IDLPIQNNFR EGLSLTEYII
SCYGARKGVV DTAVRTSDAG YLTRRLVEVV QHIVVRKKDC GTLRGILLNS NRDGRRSIQP
FSQQRLIGRV LADNLYVNMR CIATRNQDIS NELARRLVIS QVRQIYIRSP LTCKSMFCIC
QLCYGWNLTY NHLVELGEAV GIIAGQSIGE PGTQLTLRTF HTGGVFTGDI AEHIRTPFNG
IIKFDEDLVY ATRTRHGHPA WLCQDNLPVS IESKEKIHNF IVPAQSLLLV QNNQYVGSKQ
VIGQIRTTKS PLKERVKKPI YSNLDGELHW DTTVRHLSEH IHNNIHRVVK SGHIWILSGK
LHNLSKTSLF FYQNQDKIHV QSPSITNYKS LPNSSEKDTK RDLDFIDSNI AKKESQTSNF
GFSFSKITPN PSDSTNILSN IELKRNRRKN RVILLLGRVK SRYRKKSYSS FVLDIPNNGI
LNQGDILAIS ESPQYQTEIS GIIKYGTIKV DSVGKREYIG DNEKTTTFRS RYKILKGGNF
FLIPEEVYTI YQPSSHIFVS NNSIIEPGTQ ITFNITSRGG GLVQIKKVRK IFEIRILPGN
IYYPKRLHKI SKQTDILIPP GQKIFDEFKS DNWIYLQWIT PYRKKTFLPV RPVLEYIISN
HSFIEISSTF TYSRYRDIIQ VQAAEYIFYE DGEEVRIRNN TSSIQLVQTC LVVNWKEPSI
VRVAYVSPIE IRFNNILKTF LQISLIEFSN AYVEKGNSRA SLKRLFSNQP FYSNNANNHL
LSQFPINHPG VVRRLSDQGT DRSFVVLLPS DSYQSFYSPC HDGRNRRKQF VESRYKISFC
EKEISERSNT LFLSFNRKKF LKLKEKLTNS FSSFDHHCMM QTTERLGLLG TVHSIAHYSL
YSHRIINKNF FLSKDLDIDN SRAISQNSNW YILDENRVIC GFDLIDSTQE DLSNRPHYLS
VPCDVIIPFV NLGQFICEGV SLYEHETSYQ SGQIIAIYQE SVMIRLSKPY LATKGATVHS
NYGDILKEGD TLITLIYERL RSGDIIQGLP KVEQLLEARS INSVSLDLEK NFLFWNNTMI
RLVGNFWSHF LGVKTSIEQC QLFLVNKVQK VYRSQGVQIS DKHIEIIVRQ MTSKVITLED
GMANVFSPGE LIELSRAQRM NRALKESISH KPIVLGMTKA SLNTTSFISE ASFQETTRVL
ARAALQGRID WLKGLKENVV PGSVVPTGTG SREVICQIDI EKRKELGLLK TKNNKIFIRK
IRDIFFYHEK VSISKNPKYI HMRLKKPVLK EINI
