ID   RPOC2_ATRBE             Reviewed;        1389 AA.
AC   Q8S8Y1;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN   Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS   Atropa belladonna (Belladonna) (Deadly nightshade).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Hyoscyameae; Atropa.
OX   NCBI_TaxID=33113;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Ab5p(kan);
RX   PubMed=12200487; DOI=10.1093/oxfordjournals.molbev.a004222;
RA   Schmitz-Linneweber C., Regel R., Du T.G., Hupfer H., Herrmann R.G.,
RA   Maier R.M.;
RT   "The plastid chromosome of Atropa belladonna and its comparison with that
RT   of Nicotiana tabacum: the role of RNA editing in generating divergence in
RT   the process of plant speciation.";
RL   Mol. Biol. Evol. 19:1602-1612(2002).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC       Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC       Rule:MF_01324}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR   EMBL; AJ316582; CAC88034.1; -; Genomic_DNA.
DR   RefSeq; NP_783222.2; NC_004561.1.
DR   AlphaFoldDB; Q8S8Y1; -.
DR   GeneID; 806554; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.132.30; -; 1.
DR   Gene3D; 1.10.274.100; -; 1.
DR   HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR   InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR   InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR   InterPro; IPR007083; RNA_pol_Rpb1_4.
DR   InterPro; IPR007081; RNA_pol_Rpb1_5.
DR   InterPro; IPR038120; Rpb1_funnel_sf.
DR   PANTHER; PTHR19376; PTHR19376; 1.
DR   Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR   Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR   TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW   Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT   CHAIN           1..1389
FT                   /note="DNA-directed RNA polymerase subunit beta''"
FT                   /id="PRO_0000067916"
FT   BINDING         224
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         295
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         302
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         305
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ   SEQUENCE   1389 AA;  156857 MW;  987B9E096CB4B1D5 CRC64;
     MEVLMAERAN LVFHNKAIDG TAMKRLISRL IEHFGMAYTS HILDQVKTLG FQQATATSIS
     LGIDDLLTIP SKGWLVQDAE QQSLILEKHH HYGNVHAVEK LRQSIEIWYA TSEYLRQEMN
     PNFRMTDPFN PVHIMSFSGA RGNASQVHQL VGMRGLMSDP QGQMIDLPIQ SNLREGLSLT
     EYIISCYGAR KGVVDTAVRT SDAGYLTRRL VEVVQHIVVR RTDCGTARGI SVSPRNGMMP
     ERIFSQTLIG RVLADDIYMG PRCIATRNQD IGIGLVNRFI TFRAQPISIR TPFTCRSTSW
     ICRLCYGRSP THGDLVELGE AVGIIAGQSI GEPGTQLTLR TFHTGGVFTG GTAEHVRAPS
     NGKIKFNEDL VHPTRTRHGH PAFLCSIDLY VTIESEDILH NVNIPPKSLL LVQNDQYVES
     EQVIAEIRAG ISTLNFKEKV RKHIYSDSDG EMHWSTDVYH APEFTYGNVH LLPKTSHLWI
     LLGGPCKSSL VYLSIHKDQD QMNAHSLSGK QRYTSNLSVT NDQARQKLFS SGFSGKKEDR
     IPDYSDLNRI ICTGQYNLVY SPILHENSDL LSKRRRNKFI IPLHSIQELE NELMPCSGIS
     IEIPVNGIFR RNSILAYFDD PRYRRKSSGI IKYGTIETHS VIKKEDLIEY RGVKEFRPKY
     QMKVDRFFFI PEEVHILPGS SSIMVRNNSI VGVDTQITLN LRSRVGGLVR VERKKKRIEL
     KIFSGDIHFP GETDKISRHT GLLIPPGTGK INSKESKKVK KWIYVQRITP SKKKFFVLVR
     PVVTYEITDG INFETLFPPD PLQERDNVQL RIVNYILYGN GKPIRGISDT SIQLVRTCLV
     LNWNQDKKSS SCEEARASFV EIRTNGLIRH FLRINLVKSP ISYIGKRNDP SGSGLLSDNG
     SDCTNINPFS SIYSYSKAKI QQSLNQPQGT IHTLLNRNKE CQSLIILSSA NCSRMGTLKY
     HSVIKDSIKK DPLIPIRNSL GPLGTCLPIE NFYSSYRLIT HNQILVTNYL QLDNLKQTFQ
     VIKFKYYLMD ENGKIFNPDP CRNIILNPFN LNWYFLHHNY CEETSKIISL GQFICENVCI
     AKNGPPLKSG QVILVQVDSI VIRSAKPYLA TPGATVHGHY GETLYEGDTL VTFIYEKSRS
     GDITQGLPKV EQVLEVRSID SISMNLEKRV EGWNKCITRI LGIPWGFLIG AELTIAQSRI
     SLVNKIQQVY RSQGVQIHNR HIEIIVRQIT SKVLVSEDGM SNVFSPGELI GLLRAERMGR
     ALEEAICYRV VLLGITRASL NTQSFISEAS FQETARVLAK AALRGRIDWL KGLKENVVLG
     GVIPVGTGFK GLVHPSKQHN NIPLETKKKN LFEGEMRDIL FHHRKLFDSC LSKKFHDIPE
     QSFIGFNDS