ID   RPOC2_BUXMI             Reviewed;        1391 AA.
AC   A6MM26;
DT   04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN   Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS   Buxus microphylla (Littleleaf boxwood) (Japanese boxwood).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Buxales; Buxaceae; Buxus.
OX   NCBI_TaxID=153571;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17644003; DOI=10.1016/j.ympev.2007.06.004;
RA   Hansen D.R., Dastidar S.G., Cai Z., Penaflor C., Kuehl J.V., Boore J.L.,
RA   Jansen R.K.;
RT   "Phylogenetic and evolutionary implications of complete chloroplast genome
RT   sequences of four early-diverging angiosperms: Buxus (Buxaceae),
RT   Chloranthus (Chloranthaceae), Dioscorea (Dioscoreaceae), and Illicium
RT   (Schisandraceae).";
RL   Mol. Phylogenet. Evol. 45:547-563(2007).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC       Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC       Rule:MF_01324}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR   EMBL; EF380351; ABQ45239.1; -; Genomic_DNA.
DR   RefSeq; YP_001294174.1; NC_009599.1.
DR   AlphaFoldDB; A6MM26; -.
DR   GeneID; 5236945; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.132.30; -; 1.
DR   Gene3D; 1.10.274.100; -; 1.
DR   HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR   InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR   InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR   InterPro; IPR007083; RNA_pol_Rpb1_4.
DR   InterPro; IPR007081; RNA_pol_Rpb1_5.
DR   InterPro; IPR038120; Rpb1_funnel_sf.
DR   PANTHER; PTHR19376; PTHR19376; 1.
DR   Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR   Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR   TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW   Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT   CHAIN           1..1391
FT                   /note="DNA-directed RNA polymerase subunit beta''"
FT                   /id="PRO_0000353548"
FT   BINDING         224
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         295
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         302
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         305
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ   SEQUENCE   1391 AA;  158695 MW;  F504ACC79CFA820E CRC64;
     MEVLMAERAD LVFHNKVIDG TAMKRLISRL IDHFGMAYTS HILDQVKTLG FQQATATSIS
     LGIDDLLTTP SKRWLVQDAE QQSFILEKHH HYGNVHAVEK LRQSIEIWYA TSEYLRQEMN
     TNFRMTDPSN PVHIMSFSGA RGNPSQVHQL VGMRGLMSDP QGQMIDLPIQ SNLREGLSLT
     EYIISCYGAR KGVVDTAVRT SDAGYLTRRL VEVVQHIVVR RTDCGTIRGI YVTSRNGMMT
     ERIFIQTLIG RVLADDIYMG PRCIATRNQD VGIELVNRFI TFRAQPISIR TPFTCRSTSW
     ICRLCYGRSP THGDLVELGE AVGIIAGQSI GEPGTQLTLR TFHTGGVFTG GTAEHVRAPF
     NGKIKFNEDL VHPTRTRHGH PAFLCYIDLY VTIESQDIIH NVNIPQKSFL LVQNNQYVES
     EQVIAEIRAE TSTFNFKERV RKHIYSDSEG EMHWSTDVYH APEYTYGNVH LLPKTSHLWV
     LSGGPCRSRI VPFSLHKDQD QMNVHSLSAE RRYISNLSAT NDQVRHKLFS SDPSGKRGGS
     ILDYSGPNRI ICNGHLNFIY PTILHENSDL LAKRRRNRFI LPLQSNQERE KELMPCSGIS
     IEIPINGIFR RNSILAYFDD PLYRRKSSGI TKYGTIEVHS IVKKEDLIEY RGAKEFRSKY
     QMKVDRFFFI PEEVHILPGS SYIMVRNNSI IGVDTRITLN IRSRVGGLVR VERKKKNIEL
     KIFSGDIHFP GETDKISRHS GILIPPGTEK KKSKESKKKL KNWIYVQRIT PTKKKYFVLV
     RPVVTYEIAD GINLVTLFPQ DLLQERDDVQ LRVVNYILYE NGKPIRGISD TSIQLVRTCL
     VLNCDQYKNG SSMEEIHASF VEVRTNGLIR YFLRMDLVKS PISYIVKRND PSSSGLIPDN
     GSDRNNINPF YSIYYKARIQ QSLSQHQGTI RTLLNRNKEC QSLIILSSSN CSRMHPFNGL
     KYHNVTKESS KGDPLIPIKN WLGPLGTVPK IANFYSFYHL ITHNQILVNK YLILNNLKQI
     FQVLKYYLMD ENGRIYNPDR CRNIIFNPFN FNWYFLHHDY CEETSTIISP GQFICENVCI
     SKNGPHLKSG QVLIVHIDSL VIRSAKPYLA TPGATVHGHY GEILYEGDTL VTFIYEKSRS
     GDITQGLPKV EQVLEVRSID SISMNLEKRV EGWNERIPRI LGIPWGFLIG AELTIAQSRI
     SLVNKIQKVY RSQGVQIHNR HIEIIVRQIT SKVLVSEEGM SNVFSPGELI GLLRAERTGR
     ALEEAICYRA VLLGITRASL NTQSFISEAS FQETARVLAK AALRGRIDWL KGLKENVVLG
     GLIPVGTGFK VLVHRSRQHN NIPLETKKKN IFEGEMRDIL FHHRKFKLLN YCIPKNFHDT
     PEQSFTGFND S